Validity and reliability of a new instrument for the evaluation of dental collaboration in disabled people

Background: nowadays, oral health in people with disabilities is an important topic. The phsychological and behavioural problems of these people, their difficulties with environmental adaptations and the absence of any traditional communication determine the compliance needed for treatment The aim of this work was to test the validity and reliability of an original questionnaire that could become an instrument assessing the individual features in people with mental retardation and other developmental disabilities at the time of dental treatment. Methods: it was created a questionnaire with standardised answers regarding four specific areas: neuropsychology, emotional-affect, autonomy and environmental resources. The questionnaire was completed by 63 patients from three different institutes (two rehabilitation institutes and an Institute of Dentistry for patients with special needs). To analyse the answers, each item was transformed into a numeric value. A value of 1 was displayed as the minimum while 4 represented full possession of the considered skills. A total of 17 variables were analysed with descriptive statistics and multivariate analysis. Internal consistency reliability was measured using Cronbach’s alpha. Furthermore, an analysis on convergent/discriminant validity was provided. Results: all variables were positively correlated. The most significant were “guidance”, “communication”, “sociability”, “view”, “hearing” and “feeding”. Items like “self-control”, “equanimity”, “problematic behaviour”, “extroversion” and “autonomy” offered vague and less significant information in identifying the patient’s collaboration level. Variables like “evaluation by the compiler about the patient’s collaboration”, “previous dental experiences” and “attendant” were confirmed. Cronbach’s alpha was 0.77 (standardized result), which meet the a priori criterion of 0.90≥alpha≥0.70. Conclusions: the instrument was statistically tested and seems to be adequate to estimate the collaboration level of disabled patients. It is called CLEQ (Collaboration Level Evaluation Questionnaire). Further trials are warranted to confirm its validity and benefits.&nbsp

Non-pegylated liposomal doxorubicin plus ifosfamide in metastatic soft tissue sarcoma: results from a phase-II trial

Non-pegylated liposomal doxorubicin (NPLD) has demonstrated antitumour activity equivalent to conventional doxorubicin and a significantly lower risk of cardiotoxicity. This phase II trial was performed to evaluate the activity and the safety of NPLD and ifosfamide combination in patients with metastatic soft tissue sarcoma

Fondazione Italiana Linfomi (FIL) expert consensus on the use of intensity-modulated and image-guided radiotherapy for Hodgkin's lymphoma involving the mediastinum

Advances in therapy have resulted in improved cure rates and an increasing number of long-term Hodgkin's lymphoma (HL) survivors. However, radiotherapy (RT)-related late effects are still a significant issue, particularly for younger patients with mediastinal disease (secondary cancers, heart diseases). In many Centers, technological evolution has substantially changed RT planning and delivery. This consensus document aims to analyze the current knowledge of Intensity-Modulated Radiation Therapy (IMRT) and Image-Guided Radiation Therapy (IGRT) for mediastinal HL and formulate practical recommendations based on scientific evidence and expert opinions

Lmx1a-Dependent Activation of miR-204/211 Controls the Timing of Nurr1-Mediated Dopaminergic Differentiation

The development of midbrain dopaminergic (DA) neurons requires a fine temporal and spatial regulation of a very specific gene expression program. Here, we report that during mouse brain development, the microRNA (miR-) 204/211 is present at a high level in a subset of DA precursors expressing the transcription factor Lmx1a, an early determinant for DA-commitment, but not in more mature neurons expressing Th or Pitx3. By combining different in vitro model systems of DA differentiation, we show that the levels of Lmx1a influence the expression of miR-204/211. Using published transcriptomic data, we found a significant enrichment of miR-204/211 target genes in midbrain dopaminergic neurons where Lmx1a was selectively deleted at embryonic stages. We further demonstrated that miR-204/211 controls the timing of the DA differentiation by directly downregulating the expression of Nurr1, a late DA differentiation master gene. Thus, our data indicate the Lmx1a-miR-204/211-Nurr1 axis as a key component in the cascade of events that ultimately lead to mature midbrain dopaminergic neurons differentiation and point to miR-204/211 as the molecular switch regulating the timing of Nurr1 expression

Lmx1a-Dependent Activation of miR-204/211 Controls the Timing of Nurr1-Mediated Dopaminergic Differentiation

The development of midbrain dopaminergic (DA) neurons requires a fine temporal and spatial regulation of a very specific gene expression program. Here, we report that during mouse brain development, the microRNA (miR-) 204/211 is present at a high level in a subset of DA precursors expressing the transcription factor Lmx1a, an early determinant for DA-commitment, but not in more mature neurons expressing Th or Pitx3. By combining different in vitro model systems of DA differentiation, we show that the levels of Lmx1a influence the expression of miR-204/211. Using published transcriptomic data, we found a significant enrichment of miR-204/211 target genes in midbrain dopaminergic neurons where Lmx1a was selectively deleted at embryonic stages. We further demonstrated that miR-204/211 controls the timing of the DA differentiation by directly downregulating the expression of Nurr1, a late DA differentiation master gene. Thus, our data indicate the Lmx1a-miR-204/211-Nurr1 axis as a key component in the cascade of events that ultimately lead to mature midbrain dopaminergic neurons differentiation and point to miR-204/211 as the molecular switch regulating the timing of Nurr1 expression

miR-34b/c Regulates Wnt1 and Enhances Mesencephalic Dopaminergic Neuron Differentiation

The differentiation of dopaminergic neurons requires concerted action of morphogens and transcription factors acting in a precise and well-defined time window. Very little is known about the potential role of microRNA in these events. By performing a microRNA-mRNA paired microarray screening, we identified miR-34b/c among the most upregulated microRNAs during dopaminergic differentiation. Interestingly, miR-34b/c modulates Wnt1 expression, promotes cell cycle exit, and induces dopaminergic differentiation. When combined with transcription factors ASCL1 and NURR1, miR-34b/c doubled the yield of transdifferentiated fibroblasts into dopaminergic neurons. Induced dopaminergic (iDA) cells synthesize dopamine and show spontaneous electrical activity, reversibly blocked by tetrodotoxin, consistent with the electrophysiological properties featured by brain dopaminergic neurons. Our findings point to a role for miR-34b/c in neuronal commitment and highlight the potential of exploiting its synergy with key transcription factors in enhancing in vitro generation of dopaminergic neurons.Peer reviewe

miR-218 promotes dopaminergic differentiation and controls neuron excitability and neurotransmitter release through the regulation of a synaptic-related genes network

In the brain microRNAs (miRNAs) are believed to play a role in orchestrating synaptic plasticity at a higher-level by acting as an additional mechanism of translational regulation, alongside the mRNAs/polysome system. Despite extensive research, our understanding of the specific contribution of individual miRNA to the function of dopaminergic neurons (DAn) remains limited. By performing a dopaminergic-specific miRNA screening, we have identified miR-218 as a critical regulator of DAn activity in male and female mice. We have found that miR-218 is specifically expressed in mesencephalic DAn and is able to promote dopaminergic differentiation of embryonic stem cells and functional maturation of transdifferentiated induced DA neurons. Midbrain-specific deletion of both genes encoding for miR-218 (referred to as miR-218-1 and mir218-2) affects the expression of a cluster of synaptic-related mRNAs and alters the intrinsic excitability of DAn, as it increases instantaneous frequencies of evoked action potentials, reduces rheobase current, affects the ionic current underlying the action potential after hyperpolarization phase and reduces dopamine efflux in response to a single electrical stimulus. Our findings provide a comprehensive understanding of the involvement of miR-218 in the dopaminergic system and highlight its role as a modulator of dopaminergic transmission

Building Greener Cities Together: Urban Afforestation Requires Multiple Skills to Address Social, Ecological, and Climate Challenges

Urban afforestation is increasingly regarded as a key strategy for fostering biodiversity to restore and enhance the ecosystem services needed to counteract the effects of climate change in built-up areas. In Italy, several experimental afforestation projects have been launched as part of the National Recovery and Resilience Plan (NRRP), focusing on cities or metropolitan areas such as Milan, Rome, Pistoia and Campobasso. These projects follow a multidisciplinary approach, integrating botanists, foresters, urban planners, landscape architects and remote sensing specialists. The goal is to address the challenging complexity of urban forest restoration through reforestation and afforestation actions. Key innovations include the integration of transdisciplinary methodologies (landscape analysis, landscape design, forest and plant ecology) with the application of advanced remote sensing technologies and participatory community engagement frameworks to address ecological and social challenges. Experimental plots have been set up across various urban areas, testing a range of planting schemes to maximise climate change resilience and ensure long-term ecological sustainability. Emphasis has been placed on selecting drought-tolerant and thermophilic species that are better adapted to widespread warming and local urban heat islands. Biodiversity strips with perennial flowers for insects, shrubs with berries for birds and nests for wild bees and vertebrates have been set up to enhance biodiversity in new afforestation areas. Advanced monitoring tools, such as Light Detection and Ranging (LiDAR) and multi-sensor drones, have been employed alongside field observations to assess forest growth, species survival, structural complexity and biodiversity enhancement over time. Historical analyses of landscape patterns and ecological connectivity over the past 200 years, along with evaluations of afforestation projects from the last 70 years, have provided critical insights into the successes and challenges of previous interventions, serving as a guide for future efforts. By focusing on ecological connectivity, the integration of afforested areas into the urban matrix, and citizen engagement, the current project aims to align urban forestry efforts with sustainable development goals. This comprehensive project framework addresses environmental restoration and the social and aesthetic impacts on local communities, contributing to the overall resilience and well-being of urban and peri-urban ecosystems

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

ReSurveyEurope : A database of resurveyed vegetation plots in Europe

Aims: We introduce ReSurveyEurope — a new data source of resurveyed vegetation plots in Europe, compiled by a collaborative network of vegetation scientists. We de- scribe the scope of this initiative, provide an overview of currently available data, governance, data contribution rules, and accessibility. In addition, we outline further steps, including potential research questions. Results: ReSurveyEurope includes resurveyed vegetation plots from all habitats. Version 1.0 of ReSurveyEurope contains 283,135 observations (i.e., individual sur- veys of each plot) from 79,190 plots sampled in 449 independent resurvey projects. Of these, 62,139 (78%) are permanent plots, that is, marked in situ, or located with GPS, which allow for high spatial accuracy in resurvey. The remaining 17,051 (22%) plots are from studies in which plots from the initial survey could not be exactly relocated. Four data sets, which together account for 28,470 (36%) plots, provide only presence/absence information on plant species, while the remaining 50,720 (64%) plots contain abundance information (e.g., percentage cover or cover–abun- dance classes such as variants of the Braun- Blanquet scale). The oldest plots were sampled in 1911 in the Swiss Alps, while most plots were sampled between 1950 and 2020. Conclusions: ReSurveyEurope is a new resource to address a wide range of re- search questions on fine-scale changes in European vegetation. The initiative is de- voted to an inclusive and transparent governance and data usage approach, based on slightly adapted rules of the well-established European Vegetation Archive (EVA). ReSurveyEurope data are ready for use, and proposals for analyses of the data set can be submitted at any time to the coordinators. Still, further data contributions are highly welcom