Dataset supporting Figure S3_1 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from a dorsal mount mouse with online fast-z motion correction, corresponding to Figure S3, session _1. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Dataset supporting Figure 5 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from a side mount mouse, corresponding to Figure 5. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Dataset supporting Figure S6_E235 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from the same side mount mouse as in Figures 5 and 6 (but a different session than in those Figures), corresponding to panels d-f of Figure S6. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data. Additional python files corresponding to this session can be found in the FigShare+ folder in this collection corresponding to Figure 6 (this session was used in the BSOiD model training for the session that was fit in Figure 6).Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Dataset supporting Figure 4 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from a dorsal mount mouse, corresponding to Figure 4. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Dataset supporting Figure 6 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from the same side mount mouse as in Figure 5, but corresponding to Figure 6. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data. Also contains DeepLabCut and BSOiD movies and analysis files corresponding to Figure 6 and to three other sessions from the same side mount mouse used, along with the target session, to train the BSOiD model used to fit the target session.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Dataset supporting Figure 2 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from the same dorsal mount mouse, corresponding to panels a-c and d of Figure 2, respectively. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Dataset supporting Figure S3_0 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from a dorsal mount mouse without online fast-z motion correction, corresponding to Figure S3, session _0. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Dataset supporting Figure S6_E210 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from the same dorsal mount mouse as in Figure 4 (but a different session than in that Figure), corresponding to panels a-c of Figure S6. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data. Additional python files corresponding to this session can be found in the FigShare+ folder in this collection corresponding to Figure 2.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Dataset supporting Figure 3 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from the same side mount mouse, corresponding to panels a-c and d of Figure 3, respectively. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data.Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#".Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff".Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat).In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt".Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing.Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included.Please contact the authors for any additional clarifications as needed.See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513</p

Changes in action potential shape do not explain the decrease in Na⁺ entry and Na⁺/K⁺ current overlap with increases in temperature.

<p>A. Action potential waveform occurring in the model axon at 18°C. B. Currents obtained from the model when the action potential in (A) was injected into the model, using ionic current kinetics obtained at 18°C. This result is the same as <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002456#pcbi-1002456-g001" target="_blank">Figure 1B</a>, 18°C. C–D. Running the model through the action potential waveform of (A), but with the channel kinetics of 27°C (C) or 37°C (D) results in a large decrease in Na⁺/K⁺ channel overlap (cf. B). E. Action potential waveform occurring in the model axon at 37°C. F. Injecting the action potential waveform in (E) into the model with Na⁺/K⁺ kinetics appropriate for 18°C results in a large inward Na⁺ current during the falling phase of the action potential, which overlaps extensively with the outward K⁺ current. G–H. Increasing the kinetics to those appropriate for 27°C (G) and 37°C (H) results in a large decrease in the overlap of Na⁺/K⁺ currents and the near disappearance of the Na⁺ current occurring during the falling phase of the spike (H). Dashed lines are aligned to the peak of the injected spike. See also supplemental <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002456#pcbi.1002456.s001" target="_blank">figure S1</a>.</p