The association between RANTES −28C/G polymorphism and asthma in recessive model.

<p>The association between RANTES −28C/G polymorphism and asthma in recessive model.</p

Association between RANTES Gene Polymorphisms and Asthma: A Meta-Analysis

<div><p>Background</p><p>A few recent studies have suggested that regulated on activation, normal T cell expressed and secreted (RANTES) polymorphisms (−403 G/A, −28C/G) are associated with asthma. However, there still existed studies which did not confirm these correlations.</p><p>Objective</p><p>The objective of this study was to evaluate the relationship of RANTES and asthma using a meta-analysis.</p><p>Methods</p><p>Pubmed, Embase, and Cochrane library databases were systemically searched. Data were extracted by two independent reviewers and pooled odds ratio (OR) with 95% confidence interval (CI) were calculated.</p><p>Results</p><p>Eighteen studies were enrolled, including a total of 2558 cases and 2630 controls of −403 G/A, as well as 3311 cases and 4031 controls of −28C/G in this meta-analysis. The overall ORs and 95% CIs of −403 G/A were 1.19, 1.06–1.33 (P<0.001) and 1.25, 1.03–1.51 (P = 0.933) in dominant and recessive models, respectively. The overall ORs and 95% CIs of −28G were 1.23, 1.09–1.39 (P = 0.221) and 1.76, 1.32–2.34 (P = 0.356) in dominant and recessive models, respectively. No publication bias among studies was showed.</p><p>Conclusions</p><p>This meta-analysis showed that RANTES −403 G/A polymorphism was a risk factor for asthma, while −28C/G polymorphism were not associated with asthma.</p></div

The association between RANTES-403G/A polymorphism and asthma in recessive model.

<p>The association between RANTES-403G/A polymorphism and asthma in recessive model.</p

Characteristics of included studies in the meta-analysis for the −403G/A polymorphism.

<p>Characteristics of included studies in the meta-analysis for the −403G/A polymorphism.</p

The association between RANTES-403G/A polymorphism and asthma in dominant model.

<p>The association between RANTES-403G/A polymorphism and asthma in dominant model.</p

Characteristics of included studies in the meta-analysis for the −28C/G Polymorphism.

<p>Characteristics of included studies in the meta-analysis for the −28C/G Polymorphism.</p

Controlling the Growth of Palladium Aerogels with High-Performance toward Bioelectrocatalytic Oxidation of Glucose

We report the controllable synthesis of Pd aerogels with high surface area and porosity by destabilizing colloidal solutions of Pd nanoparticles with variable concentrations of calcium ions. Enzyme electrodes based on Pd aerogels co-immobilized with glucose oxidase show high activity toward glucose oxidation and are promising materials for applications in bioelectronics

Palladium-Catalyzed Domino Carbopalladation/Carbonylative Cyclization: Synthesis of Heterocycles bearing Oxindoles and 3‑Acylbenzofuran/3-Acylindole Moieties

A novel and straightforward methodology for palladium-catalyzed carbopalladation-initiated domino carbonylative cyclization to construct bisheterocycles has been established. With TFBen as an efficient and convenient CO source, the protocol is capable of generating oxindole and 3-acylbenzofuran/3-acylindole moieties from the corresponding N-(o-iodoaryl)acrylamides and o-alkynylphenols/o-alkynylanilines with the formation of three C–C bonds and one C–O/C–N bond in a single one-step operation. A wide range of bisheterocycles bearing oxindoles and 3-acylbenzofurans/3-acylindoles were prepared in moderate to excellent yields with good functional group tolerance

Image3_The application of short and highly polymorphic microhaplotype loci in paternity testing and sibling testing of temperature-dependent degraded samples.PNG

Paternity testing and sibling testing become more complex and difficult when samples degrade. But the commonly used genetic markers (STR and SNP) cannot completely solve this problem due to some disadvantages. The novel genetic marker microhaplotype proposed by Kidd’s research group combines the advantages of STR and SNP and is expected to become a promising genetic marker for kinship testing in degraded samples. Therefore, in this study, we intended to select an appropriate number of highly polymorphic SNP-based microhaplotype loci, detect them by the next-generation sequencing technology, analyze their ability to detect degraded samples, calculate their forensic parameters based on the collected 96 unrelated individuals, and evaluate their effectiveness in paternity testing and sibling testing by simulating kinship relationship pairs, which were also compared to 15 STR loci. Finally, a short and highly polymorphic microhaplotype panel was developed, containing 36 highly polymorphic SNP-based microhaplotype loci with lengths smaller than 100 bp and Ae greater than 3.00, of which 29 microhaplotype loci could not reject the Hardy-Weinberg equilibrium and linkage equilibrium after the Bonferroni correction. The CPD and CPE of these 29 microhaplotype loci were 1-2.96E-26 and 1-5.45E-09, respectively. No allele dropout was observed in degraded samples incubated with 100°C hot water for 40min and 60min. According to the simulated kinship analysis, the effectiveness at the threshold of 4/−4 reached 98.39% for relationship parent-child vs. unrelated individuals, and the effectiveness at the threshold of 2/−2 for relationship full-sibling vs. unrelated individuals was 93.01%, which was greater than that of 15 STR loci (86.75% for relationship parent-child vs. unrelated individuals and 81.73% for relationship full-sibling vs. unrelated individuals). After combining our 29 microhaplotype loci with other 50 short and highly polymorphic microhaplotype loci, the effectiveness values at the threshold of 2/−2 were 82.42% and 90.89% for relationship half-sibling vs. unrelated individuals and full-sibling vs. half-sibling. The short and highly polymorphic microhaplotype panel we developed may be very useful for paternity testing and full sibling testing in degraded samples, and in combination with short and highly polymorphic microhaplotype loci reported by other researchers, may be helpful to analyze more distant kinship relationships.</p