MOESM2 of PTPRD-inactivation-induced CXCL8 promotes angiogenesis and metastasis in gastric cancer and is inhibited by metformin

Additional file 2: Table S1. The clinicopathological characteristics of 332 gastric cancer patients according to the PTPRD expression statuses. Table S2 Significantly altered pathways by PTPRD-knockdown in MKN74 gastric cancer cell line

MOESM1 of PTPRD-inactivation-induced CXCL8 promotes angiogenesis and metastasis in gastric cancer and is inhibited by metformin

Additional file 1. Supplementary Method

MOESM3 of PTPRD-inactivation-induced CXCL8 promotes angiogenesis and metastasis in gastric cancer and is inhibited by metformin

Additional file 3: Figure S1. PTPRD silencing promotes cellular proliferation and migragion/invasion. Figure S2. Loss of PTPRD induce upregulation of CXCL8. Figure S3. HUVECs were cultured in supplement-lacking media with or without 1 ng/ml human recombinant CXCL8 (rhCXCL8). Figure S4. (A) MKN74 cells were treated with con-siRNA or CXCL8 siRNA for 48 h. Figure S5. PTPRD promoter methylation was assessed in various gastric cancer cell lines using methylation-specific PCR assay. Figure S6. The protein and mRNA expression of PTPRD were assessed upon treatment with metformin by western blot and RT/qRT-PCR using KATOIII, GCIY, and SNU668 whose PTPRD expression was relatively spared

Additional file 1: Figure S1. of Discoidin domain receptor 1 activity drives an aggressive phenotype in gastric carcinoma

Validation of DDR1 antibody. An oligonucleotide targeting human DDR1 (5′-ACACUAAUAUAUGGACCUAGCUUGA-3′; siDDR1) was purchased from Integrated DNA Technologies (Coralville, IA). MKN45 cells in 100 μL of medium were seeded into 24 well plates and transfected with 750 ng of siDDR1 or control siRNA. DDR1 expression in siDDR1- and control siRNA-transfected cells was assessed by Western blot. (TIF 1917 kb

Additional file 2: Figure S2. of Discoidin domain receptor 1 activity drives an aggressive phenotype in gastric carcinoma

Relative ratio of cell viability. A) KATO-III and B) MKN28 cells were plated on collagen coated or non-collagen coated dishes and cell number determined on the days indicated by MTS assay. C) The effect of 7rh benzamide on KATO-III and D) MKN28 cell viability was determined by MTS assay. Cells were plated on non-coating or collagen coating dishes. The concentration of 7rh benzamide was 0, 0.06, 0.18, 0.54, 1.62 and 4.86 ΟM. (TIF 13503 kb

Additional file 1: of Targeting interleukin-6 as a strategy to overcome stroma-induced resistance to chemotherapy in gastric cancer

Supplementary Materials and Methods. (DOCX 46 kb

Additional file 4: of Targeting interleukin-6 as a strategy to overcome stroma-induced resistance to chemotherapy in gastric cancer

Figure S3. a Western blot analysis demonstrating the expression of the indicated proteins in lysates from MKN-45 cells after 5-FU (5 μM) treatment with and without CAFs and subsequently treated with Ruxolitinib (500 nM/ml). (DOCX 187 kb

Figure S4 from Inhibition of Discoidin Domain Receptor 1 Prevents Stroma-Induced Peritoneal Metastasis in Gastric Carcinoma

A, Collagen was expressed at a higher level in fibroblasts than in gastric cancer cell lines. B, Western blotting analysis showed that low-dose 7rh can reduce the phosphorylation of DDR1 as well as upregulate DDR1 levels.</p

Figure S1 from Inhibition of Discoidin Domain Receptor 1 Prevents Stroma-Induced Peritoneal Metastasis in Gastric Carcinoma

A, Screening of DDR1 and mesenchymal cell marker (-SMA and Vimentin) expression. B, Genetic inhibition of DDR1 in GC cell line.</p

Legends for Supplementary Figures from Inhibition of Discoidin Domain Receptor 1 Prevents Stroma-Induced Peritoneal Metastasis in Gastric Carcinoma

Legends for Supplementary Figures</p