1 research outputs found
Composici贸n qu铆mica y actividad antif煤ngica del l谩tex de Argemone mexicana (Cardo Santo)
Ecuador cuenta con una amplia diversidad de especies vegetales que han sido usadas como medicina tradicional, entre ellas la especie Argemone mexicana (Cardo Santo), la cual se encuentra distribuida en varias zonas de la sierra central, el l谩tex es usado en forma t贸pica, de manera directa y en emplastos, debido a que presenta propiedades antif煤ngicas. El presente trabajo de investigaci贸n se enfoca en la caracterizaci贸n e identificaci贸n de las especies qu铆micas presentes en el l谩tex de dicha especie, para lo cual se obtuvieron dos extractos (acuoso y etan贸lico). Se realizaron cortes en la planta y mediante jeringuillas esterilizadas, se extrajo el l谩tex para su posterior caracterizaci贸n por medio de un tamizaje fitoqu铆mico (an谩lisis qu铆mico referencial) y la t茅cnica de CG-EM (cromatograf铆a de gases acoplado a espectrometr铆a de masas). Paralelamente, se realizaron pruebas de bioactividad, mediante la t茅cnica de Kirby Bauer, con las cepas de hongos Botrytis cinerea y Cladoosporium spp, y con dos medios de cultivo (agar PDA y Saoubourad), donde se logr贸 comprobar la actividad antif煤ngica del extracto acuoso del l谩tex a concentraciones de 10 碌l y 20 碌l del extracto, mediante la observaci贸n evidente de la formaci贸n de halos de inhibici贸n bien definidos. Utilizando los metabolitos secundarios identificados por cromatograf铆a de gases-detector de masas se realiz贸 una comparaci贸n de sus estructuras qu铆micas y bioactividad con trabajos similares reportados en la literatura, lo que permiti贸 atribuirle la actividad antif煤ngica a los compuestos identificados, pudiendo ser responsables de la actividad biol贸gica observada.Ecuador has a wide diversity of plant species that have been used as traditional medicine, including the Argemone mexicana specie (Cardo Santo), which is distributed in various areas of the central highlands, latex is used topically, directly and in plasters, because it has antifungal properties. The present research work focuses on the characterization and identification of the chemical species present in the latex of said species, for which two extracts (aqueous and ethanolic) were obtained. Cuts were made in the plant and the latex was extracted using sterilized syringes for its subsequent characterization by means of a phytochemical screening (referential chemical analysis) and GC-MS technique (gas chromatography coupled to mass spectrometry). At the same time, bioactivity tests were carried out, using the Kirby Bauer technique, in the strains of fungi Botrytis cinerea and Cladoosporium spp, and with two culture media (PDA and Saubourad agar), where it was possible to verify the antifungal activity of the aqueous extract of the latex at concentrations of 10 碌l and 20 碌l of the extract, through the evident observation of the formation of well-defined inhibition halos. Using the secondary metabolites identified by gas chromatography-mass detector, a comparison of their chemical structures and bioactivity with similar works reported in the literature was made, which allowed attributing the antifungal activity to the identified compounds that could be responsible for the observed biological activity.