Dynamical analysis on f(R,G)f(R,\mathcal{G}) cosmology

We use a dynamical system approach to study the cosmological viability of $f(R,\mathcal{G})$ gravity theories. The method consists of formulating the evolution equations as an autonomous system of ODEs, using suitable variables. The formalism is applied to a class of models in which $f(R,\mathcal{G})\propto R^{n}\mathcal{G}^{1-n}$ and its solutions and corresponding stability are analysed in detail. New accelerating solutions that can be attractors in the phase space are found. We also find that this class of models does not exhibit a matter-dominated epoch, a solution which is inconsistent with current cosmological observations.Comment: 12 pages, 4 figures. Accepted for publication in Classical and Quantum Gravit

Chromatin dynamics of the developmentally regulated P. lividus neural alpha tubulin gene

Over 40 years ago, Allfrey and colleagues (1964) suggested that two histone modifications, namely acetylation and methylation, might regulate RNA synthesis. Nowadays it is universally accepted that activation of gene expression strictly depends on enzymatic mechanisms able to dynamically modify chromatin structure. Here, using techniques including DNaseI hypersensitive site analysis, chomatin immunoprecipitation and quantitative PCR analysis, we have analyzed the dynamics of histone post-translation modifications involved in developmentally/spatially controlled activation of the sea urchin PlTalpha2 tubulin gene. We have demonstrated that only when the PlTalpha2 core promoter chromatin is acetylated on H3K9, tri-methylated on H3K4 and not di-methylated on H3K27, RNA pol II can be enrolled. In contrast, we have shown that when chromatin is methylated both on H3K9 (me2/3) and H3K27 (me2) and mono methylated on H3K4 the promoter is not accessible to RNA pol II. Our results suggest that, during P. lividus embryogenesis, both HAT/HDAC and HMT/HDM activities, which are able to regulate accessibility of the PlTalpha2 basal promoter to RNA polymerase II, are coordinately switched-on

In silico characterization of the neural alpha tubulin gene promoter of the sea urchin embryo Paracentrotus lividus by phylogenetic footprinting

During Paracentrotus lividus sea urchin embryo development one alpha and one beta tubulin genes are expressed specifically in the neural cells and they are early end output of the gene regulatory network that specifies the neural commitment. In this paper we have used a comparative genomics approach to identify conserved regulatory elements in the P. lividus neural alpha tubulin gene. To this purpose, we have first isolated a genomic clone containing the entire gene plus 4.5 Kb of 50 upstream sequences. Then, we have shown by gene transfer experiments that its non-coding region drives the spatiotemporal gene expression corresponding substantially to that of the endogenous gene. In addition, we have identified by genome and EST sequence analysis the S. purpuratus alpha tubulin orthologous gene and we propose a revised annotation of some tubulin family members. Moreover, by computational techniques we delineate at least three putative regulatory regions located both in the upstream region and in the first intron containing putative binding sites for Forkhead and Nkx transcription factor families

Reply: Comparison of preoperative and postoperative lymphatic function is essential to understand the changes in lymphatic function

Reply to a Letter to Edito

Automated Analysis of MUTEX Algorithms with FASE

In this paper we study the liveness of several MUTEX solutions by representing them as processes in PAFAS s, a CCS-like process algebra with a specific operator for modelling non-blocking reading behaviours. Verification is carried out using the tool FASE, exploiting a correspondence between violations of the liveness property and a special kind of cycles (called catastrophic cycles) in some transition system. We also compare our approach with others in the literature. The aim of this paper is twofold: on the one hand, we want to demonstrate the applicability of FASE to some concrete, meaningful examples; on the other hand, we want to study the impact of introducing non-blocking behaviours in modelling concurrent systems.Comment: In Proceedings GandALF 2011, arXiv:1106.081

Asymmetric accidental composite dark matter

The goal of this work is to find the simplest UV completion of Accidental Composite Dark Matter Models (ACDM) that can dynamically generate an asymmetry for the DM candidate, the lightest dark baryon (DCb), and simultaneously annihilate the symmetric component. In this framework the DCb is a bound state of a confining SU(N)(DC) gauge group, and can interact weakly with the visible sector. The constituents of the DCb can possess non-trivial charges under the Standard Model gauge group. The generation of asymmetry for such candidate is a two-flavor variation of the out-of-equilibrium decay of a heavy scalar, with mass M-phi greater than or similar to 10(10) GeV. Below the scale of the scalars, the models recover accidental stability, or long-livedness, of the DM candidate. The symmetric component is annihilated by residual confined interactions provided that the mass of the DCb m(DCb) less than or similar to 75 TeV. We implement the mechanism of asymmetry generation, or a variation of it, in all the original ACDM models, managing to generate the correct asymmetry for DCb of masses in this range. For some of the models found, the stability of the DM candidate is not spoiled even considering generic GUT completions or asymmetry generation mechanisms in the visible sector

Maintenance of a Protein Structure in the Dynamic Evolution of TIMPs over 600 Million Years

Deciphering the events leading to protein evolution represents a challenge, especially for protein families showing complex evolutionary history. Among them, TIMPs represent an ancient eukaryotic protein family widely distributed in the animal kingdom. They are known to control the turnover of the extracellular matrix and are considered to arise early during metazoan evolution, arguably tuning essential features of tissue and epithelial organization. To probe the structure and molecular evolution of TIMPs within metazoans, we report the mining and structural characterization of a large data set of TIMPs over approximately 600 Myr. The TIMPs repertoire was explored starting from the Cnidaria phylum, coeval with the origins of connective tissue, to great apes and humans. Despite dramatic sequence differences compared with highest metazoans, the ancestral proteins displayed the canonical TIMP fold. Only small structural changes, represented by an α-helix located in the N-domain, have occurred over the evolution. Both the occurrence of such secondary structure elements and the relative solvent accessibility of the corresponding residues in the three-dimensional structures raises the possibility that these sites represent unconserved element prone to accept variations

Insecticidal effects of eudesmanes from Pluchea sagittalis (Asteraceae) on Spodoptera frugiperda and Ceratitis capitata

Eudesmanoids play an important role in the protection of plants against herbivores. Pluchea sagittalis (Lamarck) Cabrera (Asteraceae) is widespread in tropical South America and contains compounds that provide protection against phytophagous insects. In the present work we isolated seven sesquiterpenoids with eudesmane skeletons that were evaluated for their insecticidal activities against Spodoptera frugiperda and Ceratitis capitata, pests that cause serious damage to crops in the Argentine northwest. The Eudesmanes were incorporated at different concentrations to the diet of Spodoptera frugiperda. In the choice test, larval feeding behavior was altered. The eudesmanes 1, 5 and 7 showed the highest activity with feeding election indexes (FEI) of 50, 50, and 72 %, respectively at 200 µg/g of diet. When tested for insecticidal activity using neonate larvae with the no-choice artificial diet bioassays, eudesmane 1 was the most toxic in the larval stage (LD50 177.80 mg/g of diet). Compounds 5 lowered the percentage of adult emergence and produced the most malformations (72%) compared with control. Drastic effects were observed in the oviposition deterrence activity against C. capitata. The maximum oviposition deterrence (87 %) was recorded with eudesmane 5 at dose 30 µg/cm2 of artificial fruit. Finally, eudesmanes 6 and 7 showed significant larval and pupal mortality against the first generation larvae of viable eggs oviposited by females fed with the treated diet (100 µg / g artificial diet)