Field Research on Corruption in Private Enterprise -- Taking the "Criminal Law Amendment (12)" as an Opportunity

For a long time, the governance strategy of corruption crimes in private enterprises has been mainly based on norms and ignoring facts, which may lead to poor governance of related crimes. In terms of the governance of corruption crimes within private enterprises, the characteristics of enterprise management mode, organizational structure, and form of power distribution are ignored, which may limit the protective function of the criminal law, and even cause problems such as excessive involvement of the criminal law and abuse of rights. In terms of bribery prevention, failure to fully consider the complex forms of bribery may lead to those enterprises and their households that are still worth saving may be mired in litigation, which will further lead to negative social effects. Based on the complex reality of China's private enterprises, a localized and comprehensive corruption crime governance strategy should be put forward from the perspective of enterprises and their households

Study on De-gendering Rape Crime

At present, our criminal legislation stipulates that the object of rape crime is only adult female and female, the subject of crime in addition to joint crime and indirect crime can be female the rest of the male, this recognition is gradually behind the social practice. In addition to men and women, intersex people and transgender people are increasingly appearing in the public eye, and their rights should also be protected. Serious assaults on men and other gender people, such as rape, are not suitable for the crime of forcible indecency. Rape only protect women’s sexual autonomy to no longer meet the needs of reality, also lags behind other typical countries or regions of the act, the gender differences cannot be does not constitute a rape, the premise of any gender can be the object of crime and the crime of rape, which is reflect equality, guarantee of human rights

Establishment of a novel double-antibody sandwich fluorescence microsphere immunochromatographic test strip for rapid detection of swine acute diarrhea syndrome coronavirus (SADS-CoV) infection

IntroductionSwine acute diarrhea syndrome coronavirus (SADS-CoV) is an enveloped, positive-sense, single-stranded RNA virus that causes clinical symptoms such as vomiting and diarrhea in 10-day-old piglets. SADS-CoV has caused significant economic losses in the swine industry in southern China. Currently, no effective treatments or vaccines are available for this disease, making it crucial to establish a point-of-care testing (POCT) technology for early diagnosis and prevention.MethodsIn this study, we first validated the specificity and immunogenicity of four monoclonal antibodies (mAbs) targeting the nucleocapsid (N) protein of swine acute diarrhea syndrome coronavirus (SADS-CoV). The optimal antibody pair for constructing the fluorescent microsphere-based immunochromatographic assay (FM-ICA) was determined through systematic pairwise screening. Critical parameters of the FM-ICA test strip, including antibody labeling concentration, coating concentration, incubation time, and sample dilution ratio, were subsequently optimized. Analytical performance characteristics of the developed FM-ICA were then rigorously evaluated. Finally, clinical validation was conducted by parallel testing of 72 field samples using both FM-ICA and quantitative PCR (qPCR), followed by concordance rate analysis.ResultsFirst, we demonstrated that all four monoclonal antibodies exhibited favorable immunogenicity and specificity. Subsequently, mAb 12E1 was identified as the coating antibody, and mAb 5G12 was selected as the labeled antibody, forming the optimal combination for FM-ICA preparation. After optimization, the ideal parameters were determined: a labeling concentration of 200 μg/mg for antibodies, a coating concentration of 1 mg/mL, an incubation time of 10 min, and a dilution factor of 10. The FM-ICA exhibited outstanding specificity, sensitivity, reproducibility, and stability, achieving a maximum detectable dilution factor of 1280 and a limit of detection (LOD) of 78 PFU mL⁻¹. Finally, the concordance rate between FM-ICA and qPCR for clinical samples reached 97.22%.DiscussionThese results indicate that FM-ICA is an excellent POCT technology that can be used for the early diagnosis of SADS-CoV, providing support for disease prevention and treatment

No evidence for persistent natural plague reservoirs in historical and modern Europe

Caused by Yersinia pestis, plague ravaged the world through three known pandemics: the First or the Justinianic (6th–8th century); the Second (beginning with the Black Death during c.1338–1353 and lasting until the 19th century); and the Third (which became global in 1894). It is debatable whether Y. pestis persisted in European wildlife reservoirs or was repeatedly introduced from outside Europe (as covered by European Union and the British Isles). Here, we analyze environmental data (soil characteristics and climate) from active Chinese plague reservoirs to assess whether such environmental conditions in Europe had ever supported “natural plague reservoirs”. We have used new statistical methods which are validated through predicting the presence of modern plague reservoirs in the western United States. We find no support for persistent natural plague reservoirs in either historical or modern Europe. Two factors make Europe unfavorable for long-term plague reservoirs: 1) Soil texture and biochemistry and 2) low rodent diversity. By comparing rodent communities in Europe with those in China and the United States, we conclude that a lack of suitable host species might be the main reason for the absence of plague reservoirs in Europe today. These findings support the hypothesis that long-term plague reservoirs did not exist in Europe and therefore question the importance of wildlife rodent species as the primary plague hosts in Europe

Disrupted callosal connectivity underlies long-lasting sensory-motor deficits in an NMDA receptor antibody encephalitis mouse model

N-methyl-d-aspartate (NMDA) receptor-mediated autoimmune encephalitis (NMDAR-AE) frequently results in persistent sensory-motor deficits, especially in children, yet the underlying mechanisms remain unclear. This study investigated the long-term effects of exposure to a patient-derived GluN1-specific mAb during a critical developmental period (from postnatal day 3 to day 12) in mice. We observed long-lasting sensory-motor deficits characteristic of NMDAR-AE, along with permanent changes in callosal axons within the primary somatosensory cortex (S1) in adulthood, including increased terminal branch complexity. This complexity was associated with paroxysmal recruitment of neurons in S1 in response to callosal stimulation. Particularly during complex motor tasks, mAb3-treated mice exhibited significantly reduced interhemispheric functional connectivity between S1 regions, consistent with pronounced sensory-motor behavioral deficits. These findings suggest that transient exposure to anti-GluN1 mAb during a critical developmental window may lead to irreversible morphological and functional changes in callosal axons, which could significantly impair sensory-motor integration and contribute to long-lasting sensory-motor deficits. Our study establishes a new model of NMDAR-AE and identifies novel cellular and network-level mechanisms underlying persistent sensory-motor deficits in this context. These insights lay the foundation for future research into molecular mechanisms and the development of targeted therapeutic interventions

The Genomes of Oryza sativa: A History of Duplications

We report improved whole-genome shotgun sequences for the genomes of indica and japonica rice, both with multimegabase contiguity, or almost 1,000-fold improvement over the drafts of 2002. Tested against a nonredundant collection of 19,079 full-length cDNAs, 97.7% of the genes are aligned, without fragmentation, to the mapped super-scaffolds of one or the other genome. We introduce a gene identification procedure for plants that does not rely on similarity to known genes to remove erroneous predictions resulting from transposable elements. Using the available EST data to adjust for residual errors in the predictions, the estimated gene count is at least 38,000–40,000. Only 2%–3% of the genes are unique to any one subspecies, comparable to the amount of sequence that might still be missing. Despite this lack of variation in gene content, there is enormous variation in the intergenic regions. At least a quarter of the two sequences could not be aligned, and where they could be aligned, single nucleotide polymorphism (SNP) rates varied from as little as 3.0 SNP/kb in the coding regions to 27.6 SNP/kb in the transposable elements. A more inclusive new approach for analyzing duplication history is introduced here. It reveals an ancient whole-genome duplication, a recent segmental duplication on Chromosomes 11 and 12, and massive ongoing individual gene duplications. We find 18 distinct pairs of duplicated segments that cover 65.7% of the genome; 17 of these pairs date back to a common time before the divergence of the grasses. More important, ongoing individual gene duplications provide a never-ending source of raw material for gene genesis and are major contributors to the differences between members of the grass family

Research on Related Problems of Adult Doll Experience Store

The establishment of adult doll experience hall has aroused great controversy. Objectively speaking, this kind of experience hall has certain positive effects, but also has certain potential risks. According to the current laws and regulations, operating an adult doll experience store does not constitute a crime, nor does it constitute a general offense. In practice, the health problems of experience stores are not prominent, which can be avoided through daily business activities and supervision activities; Basically, there is no physical or psychological damage to minors. Relevant departments should treat this new industry correctly, perfect legislation and implement administration according to law

Research on Related Problems of Adult Doll Experience Store

The establishment of adult doll experience hall has aroused great controversy. Objectively speaking, this kind of experience hall has certain positive effects, but also has certain potential risks. According to the current laws and regulations, operating an adult doll experience store does not constitute a crime, nor does it constitute a general offense. In practice, the health problems of experience stores are not prominent, which can be avoided through daily business activities and supervision activities; Basically, there is no physical or psychological damage to minors. Relevant departments should treat this new industry correctly, perfect legislation and implement administration according to law.</jats:p

The effects of active play interventions on children’s fundamental movement skills: a systematic review

Abstract Background Many countries worldwide face the problem of underdeveloped fundamental movement skills (FMS) in children. Active play (AP) holds significant potential for enhancing children’s FMS based on its free-choice and unstructured nature, as well as its ease of implementation and dissemination. Therefore, the primary objective of this systematic review was to determine the effects of AP interventions on FMS in typically developing children. Methods A systematic literature search was conducted in six electronic databases, Web of Science, PubMed, Embase, Scopus, China National Knowledge Infrastructure (CNKI), and Wan Fang Data, from database inception to May 30, 2024. The main inclusion criteria for literature screening included the intervention modality being AP, the outcome indicator as FMS, and the intervention being for typically developing children. Information on countries and regions, study types, experimental designs, sample characteristics, measurement methods, and intervention effects of the included literature were extracted for the included literature. To assess the risk of bias in the literature, the RoB2.0 tool was used for RCTs, while the ROBINS-I 2.0 tool was applied to non-RCTs. Result Of the 3,672 articles retrieved, 9 studies were ultimately included in this review. The risk of bias assessment identified 3 studies as having a low risk of bias and 6 as having a moderate risk. The AP interventions in these 9 studies exhibited varying characteristics, with individual intervention durations ranging from 45 to 60 min, intervention frequencies ranging from once a week to four times per week, and intervention periods spanning from 4 weeks to 6 months. FMS were assessed using the Test of Gross Motor Development-2 (TGMD-2), the Bruininks-Oseretsky Test of Motor Proficiency (BOTMP), the Peabody Development Motor Scales-2 (PDMS-2), and the Movement Assessment Battery for Children (MABC). Regarding the effects of AP interventions on children’s FMS, seven studies reported a significant effect, while two others reported no significant effect. Conclusion Although the results of the included studies are inconsistent, AP interventions generally demonstrate a positive effect on FMS in typically developing children. To better understand the potential of AP in developing FMS in children, high-quality AP interventions and more in-depth studies across diverse populations, settings, and timeframes are needed. Additionally, greater clarity is needed regarding the components of AP interventions and their quantitative relationships with FMS efficacy