Requirement for Slit-1 and Robo-2 in zonal segregation of olfactory sensory neuron axons in the main olfactory bulb

The formation of precise stereotypic connections in sensory systems is critical for the ability to detect and process signals from the environment. In the olfactory system, olfactory sensory neurons (OSNs) project axons to spatially defined glomeruli within the olfactory bulb (OB). A spatial relationship exists between the location of OSNs within the olfactory epithelium (OE) and their glomerular targets along the dorsoventral axis in the OB. The molecular mechanisms underlying the zonal segregation of OSN axons along the dorsoventral axis of the OB are poorly understood. Using robo-2/ (roundabout) and slit-1/ mice, we examined the role of the Slit family of axon guidance cues in the targeting of OSN axons during development. We show that a subset of OSN axons that normally project to the dorsal region of the OB mistarget and form glomeruli in the ventral region in robo-2/ and slit-1/ mice. In addition, we show that the Slit receptor, Robo-2, is expressed in OSNs in a high dorsomedial to low ventrolateral gradient across the OE and that Slit-1 and Slit-3 are expressed in the ventral region of the OB. These results indicate that the dorsal-to-ventral segregation of OSN axons are not solely defined by the location of OSNs within the OE but also relies on axon guidance cues

Virtual reality as a new approach to assess cognitive decline in the elderly

Brain aging is a natural process that leads to a change in cognitive functions. Mild Cognitive Impairment (MCI) is a condition in which a person has cognitive functions that are below normal for his age. However, these deficits are not pronounced enough to confirm for the diagnosis of dementia. It is therefore important to develop new ways to assess cognitive functions in the elderly. This would indeed lead to a better identification of the cognitive losses that are related to normal or pathological aging. The objective of this study was to investigate the relevance of virtual reality as a new evaluation approach in psychology. To do this, 10 elderly people with Mild Cognitive Impairment, and 20 elderly people without cognitive problems, were compared using tests of prospective memory that were presented in a traditional way and in virtual reality. The diagnosis of MCI was made using the Montreal Cognitive Assessment (MoCA). Significant differences between the two groups were noted in virtual reality. Nevertheless, no difference was observed between the two groups with the traditional task. A significant positive correlation between the virtual reality task and the MoCA, but not between the traditional task and the MoCA, was observed. An evaluative approach based on virtual reality seems more sensitive to cognitive impairment associated with aging than an approach based on traditional neuropsychological tests.

Characterization of the K2-18 multi-planetary system with HARPS: A habitable zone super-Earth and discovery of a second, warm super-Earth on a non-coplanar orbit

The bright M dwarf K2-18 at 34 pc is known to host a transiting super-Earth-sized planet orbiting within the star's habitable zone; K2-18b. Given the superlative nature of this system for studying an exoplanetary atmosphere receiving similar levels of insolation as the Earth, we aim to characterize the planet's mass which is required to interpret atmospheric properties and infer the planet's bulk composition. We obtain precision radial velocity measurements with the HARPS spectrograph and couple those measurements with the K2 photometry to jointly model the observed radial velocity variation with planetary signals and a radial velocity jitter model based on Gaussian process regression. We measure the mass of K2-18b to be $8.0 \pm 1.9$ M$_{\oplus}$ with a bulk density of $3.7 \pm 0.9$ g/cm$^3$ which may correspond to a predominantly rocky planet with a significant gaseous envelope or an ocean planet with a water mass fraction $\gtrsim 50$%. We also find strong evidence for a second, warm super-Earth K2-18c at $\sim 9$ days with a semi-major axis 2.4 times smaller than the transiting K2-18b. After re-analyzing the available light curves of K2-18 we conclude that K2-18c is not detected in transit and therefore likely has an orbit that is non-coplanar with K2-18b. A suite of dynamical integrations with varying simulated orbital eccentricities of the two planets are used to further constrain each planet's eccentricity posterior from which we measure $e_b < 0.43$ and $e_c < 0.47$ at 99% confidence. The discovery of the inner planet K2-18c further emphasizes the prevalence of multi-planet systems around M dwarfs. The characterization of the density of K2-18b reveals that the planet likely has a thick gaseous envelope which along with its proximity to the Solar system makes the K2-18 planetary system an interesting target for the atmospheric study of an exoplanet receiving Earth-like insolation.Comment: 13 pages, 8 figures including 4 interactive figures best viewed in Adobe Acrobat. Submitted to Astronomy & Astrophysics. Comments welcom

Development of a toolkit for a mentoring program

The mentoring kit for a mentoring program provides the mentors with the necessary resources and tools to help to mentees and teams to understand, apply and integrate their strengths in their respective roles. The tools of the kit offer a working model with the mentee to develop an effective strategy that improves his/her performance through development based on strengths. One of the most effective methods for managing and developing talent within students are mentoring programs. These programs provide a vehicle in which knowledge and wisdom is shared while creating an environment for learning and growth. Novice mentors could benefit from a toolkit to help structure effective mentoring programs. This article describes such a toolkit to provide mentors with the necessary resources and tools to help mentees and teams to understand, apply and integrate their strengths in their respective roles. The objectives of this toolkit are: 1. Deliver models and structures so that the mentee has the ability to: - Develop transformational, theoretical and experiential learning processes. - Develop, evaluate and optimize your resources to function with greater creativity, prominence, leadership and proactivity. 2. Stimulate the development of skills that provide innovative perspectives. 3. Learn to apply the tools and skills acquired in the educational field to: - Understand and diagnose situations in context. - Develop intervention plans with his/her mentor adjusted to the needs and expectations of themselves. - Generate spaces for the identification of barriers and conflicts in his/her processes. - Stimulate actions to overcome challenges or opportunities. – Effective accompaniment of the mentees to reach their goals. Additionally, the tools included in the kit offer a working model for the mentee to develop an effective strategy that improves his/her performance through development based on strengths. This article presents the importance of the use of mentoring tools, under the guidelines of the mentoring toolkit design. This article presents the importance of the use of mentoring tools, under the guidelines of the mentoring toolkit design. This paper reflects simple tools that can be used in a systematic way so that in the mentoring process the participant can perform the most difficult task of all, that of investigating themselves and at the same time the mentor can count on valuable data to be able to facilitate the work. Future research will focus on the evaluation of the toolkit

Low Energy Electron Point Projection Microscopy of Suspended Graphene, the Ultimate "Microscope Slide"

Point Projection Microscopy (PPM) is used to image suspended graphene using low-energy electrons (100-200eV). Because of the low energies used, the graphene is neither damaged or contaminated by the electron beam. The transparency of graphene is measured to be 74%, equivalent to electron transmission through a sheet as thick as twice the covalent radius of sp^2-bonded carbon. Also observed is rippling in the structure of the suspended graphene, with a wavelength of approximately 26 nm. The interference of the electron beam due to the diffraction off the edge of a graphene knife edge is observed and used to calculate a virtual source size of 4.7 +/- 0.6 Angstroms for the electron emitter. It is demonstrated that graphene can be used as both anode and substrate in PPM in order to avoid distortions due to strong field gradients around nano-scale objects. Graphene can be used to image objects suspended on the sheet using PPM, and in the future, electron holography

The critical velocity effect as a cause for the H\alpha emission from the Magellanic stream

Observations show significant H\alpha-emissions in the Galactic halo near the edges of cold gas clouds of the Magellanic Stream. The source for the ionization of the cold gas is still a widely open question. In our paper we discuss the critical velocity effect as a possible explanation for the observed H\alpha-emission. The critical velocity effect can yield a fast ionization of cold gas if this neutral gas passes through a magnetized plasma under suitable conditions. We show that for parameters that are typical for the Magellanic Stream the critical velocity effect has to be considered as a possible ionization source of high relevance.Comment: 9 pages, 2 figures. accepted, to appear in The Astrophysical Journa

Nanoscale structuring of tungsten tip yields most coherent electron point-source

This report demonstrates the most spatially-coherent electron source ever reported. A coherence angle of 14.3 +/- 0.5 degrees was measured, indicating a virtual source size of 1.7 +/-0.6 Angstrom using an extraction voltage of 89.5 V. The nanotips under study were crafted using a spatially-confined, field-assisted nitrogen etch which removes material from the periphery of the tip apex resulting in a sharp, tungsten-nitride stabilized, high-aspect ratio source. The coherence properties are deduced from holographic measurements in a low-energy electron point source microscope with a carbon nanotube bundle as sample. Using the virtual source size and emission current the brightness normalized to 100 kV is found to be 7.9x10^8 A/sr cm^2

The Tyrosine Kinase Csk Dimerizes through Its SH3 Domain

The Src family kinases possess two sites of tyrosine phosphorylation that are critical to the regulation of kinase activity. Autophosphorylation on an activation loop tyrosine residue (Tyr 416 in commonly used chicken c-Src numbering) increases catalytic activity, while phosphorylation of a C-terminal tyrosine (Tyr 527 in c-Src) inhibits activity. The latter modification is achieved by the tyrosine kinase Csk (C-terminal Src Kinase), but the complete inactivation of the Src family kinases also requires the dephosphorylation of the activation loop tyrosine. The SH3 domain of Csk recruits the tyrosine phosphatase PEP, allowing for the coordinated inhibition of Src family kinase activity. We have discovered that Csk forms homodimers through interactions mediated by the SH3 domain in a manner that buries the recognition surface for SH3 ligands. The formation of this dimer would therefore block the recruitment of tyrosine phosphatases and may have important implications for the regulation of Src kinase activity

Dynamic splinting for knee flexion contracture following total knee arthroplasty: a case report

Total Knee Arthroplasty operations are increasing in frequency, and knee flexion contracture is a common pathology, both pre-existing and post-operative. A 61-year-old male presented with knee flexion contracture following a total knee arthroplasty. Physical therapy alone did not fully reduce the contracture and dynamic splinting was then prescribed for daily low-load, prolonged-duration stretch. After 28 physical therapy sessions, the active range of motion improved from -20° to -12° (stiff knee still lacking full extension), and after eight additional weeks with nightly wear of dynamic splint, the patient regained full knee extension, (active extension improved from -12° to 0°)

Classical Fields Near Thermal Equilibrium

We discuss the classical limit for the long-distance (``soft'') modes of a quantum field when the hard modes of the field are in thermal equilibrium. We address the question of the correct semiclassical dynamics when a momentum cut-off is introduced. Higher order contributions leads to a stochastic interpretation for the effective action in analogy to Quantum Brownian Motion, resulting in dissipation and decoherence for the evolution of the soft modes. Particular emphasis is put on the understanding of dissipation. Our discussion focuses mostly on scalar fields, but we make some remarks on the extension to gauge theories.Comment: REVTeX, 6 figure