3 research outputs found

    Biological processes deduced from analysis of deregulated miRNA-targets in <i>L. major</i>-infected human macrophages at 3 h post-infection.

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    <p>Yellow color gradient intensity correlates with up- or down-regulation levels. White nodes are not significantly overrepresented. The area of each node is proportional to the number of genes in the set annotated to the corresponding GO category. Interactions were visualized as a network using Cytoscape and BINGO plugin.</p

    Negative correlation between expressions of an up-regulated set of miRNAs and their targeted chemokine transcripts.

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    <p>Expression means of let-7a, miR-25, miR-26a, miR-140, miR-146a and miR-155 at 3 h and miR-23b and miR-132 at 6 h post-infection of three healthy donors (D1, D2 and D3; panel A) is negatively correlated with CCL2, CCL5, CXCL10, CXCL11 and CXCL12 mRNA mean levels at 12 and 24 h post-infection (panel B) in <i>L. major</i>-infected human macrophages. Results were expressed using the 2<sup>−ΔΔCt</sup> method.</p

    Time course of miRNA-210 and procaspase-3 expression levels in <i>L. major</i> infected human primary macrophages.

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    <p>MiR-210 expression at 3, 6, 12, and 24 h in infected cells relatively to non-infected cells (panel A), after siRNA-control or HIF-1α-silencing transfections in non-infected and infected cells (panel B). Results were expressed using the 2<sup>−ΔΔCt</sup> method. Stars (*) are indicated when results are statistically significant from control. One star indicates a <i>p</i> value <0.05; two stars indicate a <i>p</i> value <0.01 and three stars indicate a <i>p</i> value <0.001. Panel C represents abundance of pro-caspase-3 protein levels in time course parasite-infected macrophages of healthy donors revealed by western blot analysis. HSP27 was used as loading control. Ten µg of <i>L. major</i> lysate (latest lane) was used a negative control to ensure that anti-procaspase-3 antibody does not cross-react with parasite proteins. Data are representative of three independent experiments conducted on MDM derived from two to three different healthy donors. NI indicates non-infected cells and IF indicates infected cells.</p