4 research outputs found
sj-docx-2-dhj-10.1177_20552076241247374 - Supplemental material for COVID-19 surveillance based on consumer wearable devices
Supplemental material, sj-docx-2-dhj-10.1177_20552076241247374 for COVID-19 surveillance based on consumer wearable devices by Chunbo Zhang, Aijun Sun, Jiping Liao, Chunbo Zhang, Kunyao Yu, Xiaoyu Ma and Guangfa Wang in DIGITAL HEALTH</p
Characterization and Mechanism of a Novel Rice Protein Peptide (AHVGMSGEEPE) Calcium Chelate in Enhancing Calcium Absorption in Caco-2 Cells
Rice protein peptides (RPP) are a potentially valuable
source of
high-quality calcium chelating properties. However, there is a lack
of information regarding the calcium-absorption-promoting effect of
RPP and its underlying mechanism. The present study adopted molecular
docking methodologies to analyze the 10 most potent peptide segments
from RPP. Results revealed that the peptide AHVGMSGEEPE (AHV) displayed
optimal calcium binding properties (calcium-chelating capacity 55.69
± 0.66 mg/g). Quantum chemistry analysis revealed that the AHV
peptide effectively binds and forms stable complexes with calcium
via the carbonyl oxygen atoms in valine at position 3 and the carbonyl
of the C-terminal carboxyl group of glutamate at position 11. The
spectral analysis results indicated that AHV may bind to calcium through
carboxyl oxygen atoms, resulting in a transition from a smooth surface
block-like structure to a dense granular structure. Furthermore, this
study demonstrated that the 4 mmol/L AHV-Ca chelate (61.75 ±
13.23 μg/well) significantly increases calcium absorption compared
to 1 mM CaCl2 (28.57 ± 8.59 μg/well) in the
Caco-2 cell monolayer. In terms of mechanisms, the novel peptide–calcium
chelate AHV-Ca derived from RPP exerts a cell-level effect by upregulating
the expression of TRPV6 calcium-ion-channel-related genes and proteins
(TRPV6 and Calbindin-D9k). This study provides a theoretical basis
for developing functional foods with the AHV peptide as ingredients
to improve calcium absorption
sj-docx-1-dhj-10.1177_20552076241247374 - Supplemental material for COVID-19 surveillance based on consumer wearable devices
Supplemental material, sj-docx-1-dhj-10.1177_20552076241247374 for COVID-19 surveillance based on consumer wearable devices by Chunbo Zhang, Aijun Sun, Jiping Liao, Chunbo Zhang, Kunyao Yu, Xiaoyu Ma and Guangfa Wang in DIGITAL HEALTH</p
Additional file 1: of Association between socioeconomic status and post-stroke functional outcome in deprived rural southern China: a population-based study
Characteristics of the sample participants according to functional outcome. (DOCX 24 kb