Functionalized Metal–Organic Framework UiO-66-NH-BQB for Selective Detection of Hydrogen Sulfide and Cysteine

Hydrogen sulfide (H2S) is an important signaling molecule related to many diseases. Thus, H2S has a great impact on the pathological and physiological processes in biological systems. Cysteine (l-Cys) is a building block for proteins and important metabolites. To understand their roles in the physiological metabolic procedures, the measurement of the H2S level and identifying cysteine in the biological system is significant. In this study, through the functionalization of UiO-66-NH2 by 4-(2,2-dicyanoethenyl)­benzoic acid (BQB), a novel UiO-66-NH-BQB is successfully synthesized and used as a fluorescence probe to recognize and detect H2S and l-Cys. The fluorescence signals of the probe are enhanced great when it is exposed to H2S or cysteine molecules; thus, it is able to determine quantificationally the H2S concentration in an aqueous solution. The detection limitation of the UiO-66-NH-BQB to H2S concentration is found to be as low as 1.74 μM. The developed fluorescent probe based on UiO-66-NH-BQB displays a high selectivity and excellent biocompatibility, which is very promising for recognition and sensing of biothiols in organisms

Association of Urinary Metal Profiles with Altered Glucose Levels and Diabetes Risk: A Population-Based Study in China

<div><p>Background</p><p>Elevated heavy metals and fasting plasma glucose (FPG) levels were both associated with increased risk of cardiovascular diseases. However, studies on the associations of heavy metals and essential elements with altered FPG and diabetes risk were limited or conflicting. The objective of this study was to evaluate the potential associations of heavy metals and essential trace elements with FPG and diabetes risk among general Chinese population.</p><p>Methods</p><p>We conducted a cross-sectional study to investigate the associations of urinary concentrations of 23 metals with FPG, impaired fasting glucose (IFG) and diabetes among 2242 community-based Chinese adults in Wuhan. We used the false discovery rate (FDR) method to correct for multiple hypothesis tests.</p><p>Results</p><p>After adjusting for potential confounders, urinary aluminum, titanium, cobalt, nickel, copper, zinc, selenium, rubidium, strontium, molybdenum, cadmium, antimony, barium, tungsten and lead were associated with altered FPG, IFG or diabetes risk (all P< 0.05); arsenic was only dose-dependently related to diabetes (P< 0.05). After additional adjustment for multiple testing, titanium, copper, zinc, selenium, rubidium, tungsten and lead were still significantly associated with one or more outcomes (all FDR-adjusted P< 0.05).</p><p>Conclusions</p><p>Our results suggest that multiple metals in urine are associated with FPG, IFG or diabetes risk. Because the cross-sectional design precludes inferences about causality, further prospective studies are warranted to validate our findings.</p></div

Basic characteristics and clinical parameters of subjects in communities of Wuhan city, China.

<p>Abbreviations: BMI, body mass index; FPG, fasting plasma glucose; NGT, normal glucose tolerance; IFG, impaired fasting glucose. Data were presented as mean ± SD or n (%).</p><p>Basic characteristics and clinical parameters of subjects in communities of Wuhan city, China.</p

Adjusted OR and 95% CIs of diabetes risk by quartiles of urinary metals.

<p>All models were adjusted for age, gender, BMI, smoking status, pack year, alcohol status, family history of diabetes, hypertension, hyperlipidemia and urinary creatinine.</p><p>*FDR-adjusted.</p><p>Adjusted OR and 95% CIs of diabetes risk by quartiles of urinary metals.</p

Adjusted regression coefficients (95% CIs) for the association between quartiles of urinary metals and FPG.

<p>Abbreviations: FPG, fasting plasma glucose. All models were adjusted for age, gender, BMI, smoking status, pack year, alcohol status, hypertension, hyperlipidemia, family history of diabetes, anti-diabetes drugs, insulin use and urinary creatinine.</p><p>*FDR-adjusted.</p><p>Adjusted regression coefficients (95% CIs) for the association between quartiles of urinary metals and FPG.</p

Adjusted OR and 95% CIs of IFG risk by quartiles of urinary metals.

<p>Abbreviation: IFG, impaired fasting glucose. All models were adjusted for age, gender, BMI, smoking status, pack year, alcohol status, hypertension, hyperlipidemia, family history of diabetes, anti-diabetes drug, insulin use and urinary creatinine.</p><p>*FDR-adjusted.</p><p>Adjusted OR and 95% CIs of IFG risk by quartiles of urinary metals.</p

Distributions of clinical variables of participants.

<p>Distributions of clinical variables of participants.</p

Association between waist—to—height ratio and platelet indices among 17327 participants.

<p>Association between waist—to—height ratio and platelet indices among 17327 participants.</p

Association between body index mass and platelet indices among 17327 participants.

<p>Association between body index mass and platelet indices among 17327 participants.</p