Additional file 2: of Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain

Table S2. DMRs between control and aggressive bees at 120 min post interaction. (XLSX 61 kb

Additional file 1: of Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain

Table S1. DMRs between control and aggressive bees at 5 min post interaction. (XLSX 36 kb

Additional file 3: of Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain

Figure S1. Results of permutation testing for DEG/CpG overlap and comparison with Cingolani et al. 2013 [9]. (a-f) Minute 5 and Minute 120 suggestive CpGs were overlapped with gene expression differences of Aggressive and Control bees from Shpigler et al. (2017a) [4]. To test the significance of the overlap, we chose at random the same number of significantly different DEGs, checked the overlap and repeated this process 1000 times. For each plot, the blue histogram represents the distribution of random trials and the red vertical line represents the true number of overlapping DEGs with suggestive CpGs. Note that both minute 5 and minute 120 suggestive CpGs had a highly significant overlap with only minute 120 DEGs. (g-h) A similar approach was taken to test the significance of the overlap between AHB / EHB differences and minute 120 DMRs and suggestive CpGs. In both cases the true overlap was greater than the 1000 permutations. (PDF 478 kb

Additional file 6: of Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain

Table S5. Summary of samples and WGBS sequencing. (XLSX 43 kb

Additional file 5: of Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain

Table S4. Enrichments of Minute-120 suggestive CpGs and transcription factor binding sites in gene regions. (XLSX 190 kb

Additional file 4: of Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain

Table S3. Details of GO enrichments for Differentially expressed genes from Shpigler et al. 2017a [34] that overlap suggestive CpGs. (XLSX 47 kb

Cols bleus : hebdomadaire de la Marine française

06 décembre 20032003/12/06 (N2683)-2003/12/06

Additional file 15: Figure S10. of Effects of gut-derived endotoxin on anxiety-like and repetitive behaviors in male and female mice

Meta-analysis of jump time in WT males of Experiments 1 and 2. Legend: Forest plot of difference in jump time between male WT subjects gavaged with saline or LPS, measured from Experiment 1 (top green bar) and Experiment 2 (bottom green bar). The result of the meta-analysis is indicated by the red diamond. The width of the green bars and the red diamond indicate the range of the 95% confidence intervals for each, with the center representing the mean. WT males not treated with TLR4 antagonists were used for these analyses (total n = 15/group). See Additional file 18: Table S7 for statistics (PPTX 45 kb

Additional file 9: Figure S4. of Effects of gut-derived endotoxin on anxiety-like and repetitive behaviors in male and female mice

Meta-analysis of ambulatory distance in WT males of Experiments 1 and 2. Legend: Forest plot of difference in ambulatory distance between male WT subjects gavaged with saline or LPS, measured from Experiment 1 (top green bar) and Experiment 2 (bottom green bar). The result of the meta-analysis is indicated by the red diamond. The width of the green bars and the red diamond indicate the range of the 95% confidence intervals for each, with the center representing the mean. WT males not treated with TLR4 antagonists were used for these analyses (total n = 15/group). See Additional file 18: Table S7 for statistics. (PPTX 45 kb

Additional file 9: Figure S4. of Effects of gut-derived endotoxin on anxiety-like and repetitive behaviors in male and female mice

Meta-analysis of ambulatory distance in WT males of Experiments 1 and 2. Legend: Forest plot of difference in ambulatory distance between male WT subjects gavaged with saline or LPS, measured from Experiment 1 (top green bar) and Experiment 2 (bottom green bar). The result of the meta-analysis is indicated by the red diamond. The width of the green bars and the red diamond indicate the range of the 95% confidence intervals for each, with the center representing the mean. WT males not treated with TLR4 antagonists were used for these analyses (total n = 15/group). See Additional file 18: Table S7 for statistics. (PPTX 45 kb