MOESM1 of Suppressing a plant-parasitic nematode with fungivorous behavior by fungal transformation of a Bt cry gene

Additional file 1. This file includes: Methods. Figure S1. Phylogenetic analysis of Cry5Ba3 with other homologous cry5 subfamily proteins. Figure S2. Codon modification of cry5Ba3 to cry5Ba3ÎŚ. Figure S3. Fungal colony morphologies. Figure S4. Fungal colony morphologies of Botrytis cinerea transformants with different lengths of cry5Ba3ÎŚ

Screening of tea saponin-degrading strain to degrade the residual tea saponin in tea seed cake

Although tea seed cake (TSC) possesses high nutritional value, its high content of tea saponin (TS) limits its potential as feed. This study aimed to degrade TS in TSC by saponin-degrading strain and used a multistrains fermentation method to improve its nutritional value and palatability. Three saponin-degrading strains were isolated from Oleum Camelliae mill soil and identified as Citrobacter sp. FCTS301, Pantoea sp. FCTS302, and Enterobacter sp. FCTS303. Single-factor experiment showed that Citrobacter sp. FCTS301 had the highest degradation rate of TS. Response surface analysis for Citrobacter sp. FCTS301 indicated that the optimum culture conditions were as follows: initial pH of 7.2, culture temperature of 34.2 °C, inoculation amount of 7.3%, the agitation rate of 150 rpm, and the TS concentration of 10.0 g/L. Under these conditions, the maximum degradation rate was 82.6%. The fermentation process of TSC was obtained by a multistrains fermentation experiment. Considering the protein content, crude fiber degradation rate, and TS degradation rate of each group, the optimum inoculum amount of strains included Citrobacter sp. FCTS301, Aspergillus oryzae NCUF414, Saccharomyces cersvisiae NCUF306.5, and Lactobacillus plantarum NCUF201.1(5%, 0.5%, 1.0%, and 1.5%). After TS was degraded efficiently, fermented TSC can be presumed a potential feed raw material.</p

Additional file 9 of Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Additional file 9: Table S8. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the significant differentially expressed genes (SDEGs) of Chrysanthemum morifolium responding to infection with the Chinese isolate of chrysanthemum virus B (CVB-CN)

Additional file 1 of Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Additional file 1: Table S1. List of primers used in this study

Additional file 10 of Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Additional file 10: Table S9. Significant differentially expressed genes (SDEGs) in leaves of Chrysanthemum morifolium implicated in transcription and transcription regulation during infection with the Chinese isolate of chrysanthemum virus B (CVB-CN)

Additional file 4 of Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Additional file 4: Table S3. Full-length genome sequence of the Chinese isolate of chrysanthemum virus B (CVB-CN)

Additional file 7 of Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Additional file 7: Table S6. Significant differentially expressed genes (SDEGs) identified in leaves of Chrysanthemum morifolium during infection with the Chinese isolate of chrysanthemum virus B (CVB-CN)

Additional file 12 of Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Additional file 12: Table S11. Significant differentially expressed genes (SDEGs) in Chrysanthemum morifolium following infection with the Chinese isolate of chrysanthemum virus B (CVB-CN) implicated in the ethylene pathway established according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and previous studies on the model plant species Arabidopsis

Additional file 11 of Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Additional file 11: Table S10. Significant differentially expressed genes (SDEGs) in leaves of Chrysanthemum morifolium involved in ethylene pathway based on gene ontology (GO) analysis during infection with the Chinese isolate of chrysanthemum virus B (CVB-CN)

Additional file 6 of Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Additional file 6: Table S5. Summary of transcripts and unigenes assembled using the Trinity software