Additional file 1: of Deltex1 is inhibited by the Notch–Hairy/E(Spl) signaling pathway and induces neuronal and glial differentiation

Alignment of Deltex homologs and synteny comparison. a. Amino acid alignment of Drosophila, human, mouse, and zebrafish DTX1/Dtx1 sequences. Identical residues across all proteins are marked with black boxes, whereas similar residues are shown by gray boxes. The WWE domain, proline-rich domain, and RING finger motif are indicated. b. The phylogenetic tree of the Deltex protein family. Complete coding protein sequences were used for each family member. Trees were calculated using bootstrapping with 100 replicates. The phylogram shows only the sequence relationships; it does not imply absolute sequence ancestry because no ancestral relationship was assumed in the initial alignments. Genes are not drawn to scale. The initial letter “d” denotes Drosophila, “h” denotes human, “m” denotes mouse, and “z” denotes zebrafish. (TIF 893 kb

Assessment of Sustainable Well-being in the Italian Regions: An Activity Analysis Model

Applying the theoretical framework of productive analysis, the paper proposes an evaluation of regional sustainable well-being (SWB) in terms of efficiency. By means of an Activity Analysis Model (AA) (Färe et al., 1996), desirable and undesirable outcomes of development have been simultaneously used to evaluate the sustainable well-being of Italian regions. Data on equal and sustainable well-being provided by the Italian Statistical Office for the year 2010 has been used. The analysis reveals that only four regions achieve sustainable well-being, balancing socio-economic and environmental outcomes and resources. Finally, the study points out the advantages of AA for policy purposes by comparing it to a composite indicator of SWB

Factors influencing the relapse estimated by univariate and multivariate logistic regression analyses.

Factors influencing the relapse estimated by univariate and multivariate logistic regression analyses.</p

Clinicopathological features of 298 colorectal cancer patients.

Clinicopathological features of 298 colorectal cancer patients.</p

A histogram of patient LINE-1 methylation levels.

<p>A histogram of patient LINE-1 methylation levels.</p

Additional file 8: Table S1. of Deltex1 is inhibited by the Notch–Hairy/E(Spl) signaling pathway and induces neuronal and glial differentiation

Primers used for ChIP-PCR analysis. (TIF 156 kb

Kaplan-Meier disease-free survival curves.

<p>The curves depicting the effects on postoperative recurrence of LINE-1 methylation when divided into patients with “low” versus “high” methylation.</p

Additional file 7: of Deltex1 is inhibited by the Notch–Hairy/E(Spl) signaling pathway and induces neuronal and glial differentiation

Phenotypes of Dtx1 morphants were not caused by nonspecific p53 activation. Embryos coinjected with dtx1 MO and tp53 MO were compared with Dtx1 morphants, and we observed no detectable deviation from all markers tested. Compared with the controls, the injection of tp53 MO alone did not show any significant alterations, as shown by in situ hybridization (a), immunohistochemistry (b), qPCR (c), and cell count (D).*, P < 0.05; **, P < 0.01; n.s., nonsignificant. (TIF 2990 kb

Electrotaxis of CL 1-5 cells under various conditions.

<p>(A) The directedness of CL 1-5 cell electrotaxis and random migration in medium with or without 4nM Erbitux and with or without EGF stimulations. (B) The speed of CL 1-5 cell electrotaxis and random migration in medium with or without 4nM Erbitux and with or without subsequent EGF stimulation.</p

LINE-1 Methylation Status Correlates Significantly to Post-Therapeutic Recurrence in Stage III Colon Cancer Patients Receiving FOLFOX-4 Adjuvant Chemotherapy

<div><p>Background</p><p>Methylation levels of long interspersed nucleotide elements (LINE-1) are representative of genome-wide methylation status and crucial in maintaining genomic stability and expression. Their prognostic impact on colon cancer patients receiving adjuvant chemotherapy has not been well established. We evaluated the association between LINE-1 methylation status and clinicopathologic features and postoperative oncological outcomes in stage III colon cancer patients.</p><p>Materials and Methods</p><p>129 UICC stage III colon cancer patients who had received radical resection and FOLFOX adjuvant chemotherapy were enrolled. Global methylation was estimated by analyzing tumor LINE-1 methylation status using bisulfite-polymerase chain reaction (PCR) and pyrosequencing assay. Demographics, clinicopathological data, and postoperative outcomes were recorded by trained abstractors. Outcome measurements included postoperative recurrence and disease-free survival. Univariate, multivariate, and survival analyses were conducted to identify prognostic factors of oncological outcomes.</p><p>Results</p><p>The LINE-1 methylation of all 129 patients was measured on a 0–100 scale (mean 63.3; median 63.7, standard deviation 7.1), LINE-1 hypomethylation was more common in patients aged 65 years and above (61.7%±7.6% vs. 64.6±6.4, <i>p</i>=0.019) and those with post-therapeutic recurrence (61.7±7.4 vs 64.3±6.7, <i>p</i>=0.041). Considering risk adjustment, LINE-1 hypomethylation was found to be an independent risk factor of post-therapeutic recurrence (Adjusted OR=14.1, <i>p</i>=0.012). Kaplan-Meier analysis indicated that patients in the low methylation group had shorter period of disease free survival (<i>p</i>=0.01). In a stratified analysis that included 48 patients with post-therapeutic recurrence, it was found that those who experienced shorter period of disease free survival (≦6 months) appeared to have lower LINE-1 methylation levels than patients who reported of recurrence after 6 months (56.68±15.75 vs. 63.55±7.57, <i>p</i>=0.041)</p><p>Conclusion</p><p>There was a significantly greater risk of early postoperative recurrence and a shorter period of disease-free survival in Stage III colon cancer patients exhibiting LINE-1 hypomethylation status after being treated with radical resection and FOLFOX chemotherapy.</p></div