Correction: BMP-Non-Responsive Sca1⁺CD73⁺CD44⁺ Mouse Bone Marrow Derived Osteoprogenitor Cells Respond to Combination of VEGF and BMP-6 to Display Enhanced Osteoblastic Differentiation and Ectopic Bone Formation

Correction: BMP-Non-Responsive Sca1+CD73+CD44+ Mouse Bone Marrow Derived Osteoprogenitor Cells Respond to Combination of VEGF and BMP-6 to Display Enhanced Osteoblastic Differentiation and Ectopic Bone Formatio

BMPs do not enhance mineralization.

<p>The mineralization of D1 cells was measured quantitatively using alizarin red staining. The cells were stained at day 7, 14 and 21 using alizarin red (left panel). The dye was extracted and intensity of color was quantified at 405 nm using a spectrophotometer (right panel).</p

The D1 cells do not express CD105, Alk1 and Alk6 receptors.

<p>Using labeled monoclonal antibodies expression of stem cell markers (A) was determined by flow cytometry. Expression of VEGF and BMP receptors was determined by PCR followed by agarose gel electrophoresis (B).</p

The D1 cells are multipotent.

<p>The D1 cells were stained with DAPI and phalloidin to visualize cell morphology (A). Osteogenesis was assayed by staining with Alizarin Red (B) and adipogenesis was assayed by staining with Sudan IV (C).</p

Combination of VEGF and BMP-6 enhances expression of osteogenic genes.

<p>Expression of ALP (A), COL1A1 (B), runx2 (C) and osterix (D) genes were determined by real time PCR at day 7. * denotes p<0.05.</p

List of primers used in PCR.

<p>List of primers used in PCR.</p

List of antibodies used for fluorescence activated cell sorting.

<p>List of antibodies used for fluorescence activated cell sorting.</p

Enhanced expression of CADM1 in implants of D1 cells preconditioned with VEGF and BMP-6.

<p>RNA was isolated from the harvested implants and converted into cDNA. Using this cDNA as a template, mRNA expression of CADM1 was quantitatively determined in real time PCR. * denotes p<0.05.</p

BMPs do not enhance Smad 1/5/8 phosphorylation and expression of runx2 and osterix genes.

<p>mRNA levels of runx2 (A) and osterix (B) were quantified using real time PCR. Smad phosphorylation was determined by western blots (C) and band intensity was quantified using ImageJ software (D).</p

List of primers used in real time PCR.

<p>List of primers used in real time PCR.</p