Additional file 1: of Hypoglycemic encephalopathy mimicking acute ischemic stroke in clinical presentation and magnetic resonance imaging: a case report

Medical History Timeline. (DOCX 19 kb

Data_sheet_1_Sonographic Measurement of Brainstem Through the Foramen Magnum in Premature Neonates Can Predict Neurodevelopment Outcome?.docx

Background: To investigate whether serial morphometric measurements of the brainstem using high resolution trans-foramen-magnum ultrasound (US) in premature neonates correlate with neurological outcomes.Methods: Serial brain ultrasound scans were performed in 36 consecutive preterm infants born at Results: Of the 36 preterm infants born between 25 and 34 weeks of gestation, eight had significantly delayed growth profiles in both the pons and medulla and developed neurological sequelae by 5 years of age.Conclusions: Morphometric measurements of the developing brainstem using high resolution trans-foramen-magnum ultrasound (US) may help predict neurological outcome in high-risk neonates, particularly in those who are born extremely premature.</p

Additional file 1: of Identification of potential biomarkers related to glioma survival by gene expression profile analysis

Table S1. Title: 104 common survival-related genes were identified from patients with GBM and those with LGG. Description: Summarization of Cox model results for 104 survival-relevant common genes between LGG and GBM with table. (PDF 38 kb

Data_Sheet_2_First-Line A Direct Aspiration First-Pass Technique vs. First-Line Stent Retriever for Acute Ischemic Stroke Therapy: A Meta-Analysis.pdf

Background: Recent trials have proved the efficacy of mechanical thrombectomy over medical treatment for patients with acute ischemic stroke, with the balance of equivalent rates of adverse events. Stent retrievers were applied predominantly in most trials; however, the role of other thrombectomy devices has not been well validated. A direct aspiration first-pass technique (ADAPT) is proposed to be a faster thrombectomy technique than the stent retriever technique. This meta-analysis investigated and compared the efficacy and adverse events of first-line ADAPT with those of first-line stent retrievers in patients with acute ischemic stroke.Methods: A structured search was conducted comprehensively. A total of 1623 papers were found, and 4 articles were included in our meta-analysis. The Critical Appraisal Skills Programme tools were applied to evaluate the quality of studies. The primary outcome was defined as the proportion of patients with the Thrombolysis in Cerebral Ischemia (TICI) scale of 2b/3 at the end of all procedures. Secondary outcomes were the proportion of patients with functional independence (modified Rankin scale of 0–2) at the third month, the proportion of patients with the Thrombolysis in Cerebral Ischemia (TICI) scale of 2b/3 by primary chosen device, and the proportion of patients who received rescue therapies. Safety outcomes were the symptomatic intracranial hemorrhage (sICH) rate and the mortality rate within 3 months.Results: One randomized controlled trial, one prospective cohort study, and two retrospective cohort studies were included. No significant difference between these 2 strategies of management were observed in the primary outcome (TICI scale at the end of all procedures, odds ratio [OR] = 0.78), two secondary outcomes (functional independence at the third month, OR = 1.16; TICI scale by primary chosen device, OR = 1.25), and all safety outcomes (sICH rate, OR = 1.56; mortality rate, OR = 0.91). The proportion of patients who received rescue therapies was higher in the first-line ADAPT group (OR = 0.64).Conclusions: Among first-line thrombectomy devices for patients with ischemic stroke, ADAPT with the latest thrombosuction system was as efficient and safe as stent retrievers.</p

Data_Sheet_1_First-Line A Direct Aspiration First-Pass Technique vs. First-Line Stent Retriever for Acute Ischemic Stroke Therapy: A Meta-Analysis.PDF

Background: Recent trials have proved the efficacy of mechanical thrombectomy over medical treatment for patients with acute ischemic stroke, with the balance of equivalent rates of adverse events. Stent retrievers were applied predominantly in most trials; however, the role of other thrombectomy devices has not been well validated. A direct aspiration first-pass technique (ADAPT) is proposed to be a faster thrombectomy technique than the stent retriever technique. This meta-analysis investigated and compared the efficacy and adverse events of first-line ADAPT with those of first-line stent retrievers in patients with acute ischemic stroke.Methods: A structured search was conducted comprehensively. A total of 1623 papers were found, and 4 articles were included in our meta-analysis. The Critical Appraisal Skills Programme tools were applied to evaluate the quality of studies. The primary outcome was defined as the proportion of patients with the Thrombolysis in Cerebral Ischemia (TICI) scale of 2b/3 at the end of all procedures. Secondary outcomes were the proportion of patients with functional independence (modified Rankin scale of 0–2) at the third month, the proportion of patients with the Thrombolysis in Cerebral Ischemia (TICI) scale of 2b/3 by primary chosen device, and the proportion of patients who received rescue therapies. Safety outcomes were the symptomatic intracranial hemorrhage (sICH) rate and the mortality rate within 3 months.Results: One randomized controlled trial, one prospective cohort study, and two retrospective cohort studies were included. No significant difference between these 2 strategies of management were observed in the primary outcome (TICI scale at the end of all procedures, odds ratio [OR] = 0.78), two secondary outcomes (functional independence at the third month, OR = 1.16; TICI scale by primary chosen device, OR = 1.25), and all safety outcomes (sICH rate, OR = 1.56; mortality rate, OR = 0.91). The proportion of patients who received rescue therapies was higher in the first-line ADAPT group (OR = 0.64).Conclusions: Among first-line thrombectomy devices for patients with ischemic stroke, ADAPT with the latest thrombosuction system was as efficient and safe as stent retrievers.</p

Serum sex hormone levels in patients with pneumonia-related septic shock.

<p>(A) Estradiol and (B) progesterone and (C) testosterone levels in patients with pneumonia-associated septic shock, with survivors at day 28 identified by open bars, and non-survivors at day 28 by shaded bars. Medians and interquartile ranges (IQR) are shown above each plot. Statistical significance was determined with the two-sided Mann-Whitney U test. Extreme values are not shown.</p

Study profile demonstrating the number of cases and reasons for exclusion.

<p>Study profile demonstrating the number of cases and reasons for exclusion.</p

CCN1-induced OSM expression involved FAK signal transduction.

<p>(A) Osteoblasts were pretreated with FAK inhibitor (FAKi) (10 µM) or transfected with FAK siRNA (2 µm) for 24 h, then stimulated by CCN1, OSM expression gauged by qPCR. (B) Cells were transfected with FAK siRNA (2 µm) or pretreated with FAKi, protein level of FAK measured by western blot (upper-panel), OSM expression rated by ELISA assay (lower-panel). (C) Cells pretreated with FAKi for 30 min were stimulated with CCN1, protein level of OSM was measured by western blot. (D) Osteoblasts were incubated with CCN1 in time intervals, and p-FAK expression derived by western blot. (E&F) Cells pretreated with αvβ3, αvβ5, α5β1, and α6β1 antibody for 30 min were stimulated with CCN1, p-FAK expression rated by western blot. Data represent mean ± S.E. *, p<0.05 compared with control; #, p<0.05 compared with CCN1-treated group.</p

NF-κB is involved in CCN1-induced OSM production in osteoblasts.

<p>(A) Osteoblasts were pretreated with PDTC (10 µM) and TPCK (10 µM) or transfected with p65 siRNA (2 µm) followed by stimulation with CCN1, mRNA expression of OSM analyzed by qPCR. (B) Cells were transfected with p65 siRNA or pretreated with PDTC and TPCK, the protein level was measured by western blot (upper-panel), and OSM expression was measured by ELISA assay (lower-panel). (C) Cells were pretreated with PDTC and TPCK for 30 min followed by stimulation with CCN1, the protein level of OSM was measured by western blot. (D) Cells were incubated with CCN1 in time intervals, p-p65 expression evaluated by western blot. (E) Cells were pretreated with FAKi, PP2, Wortmannin or Ly294002 for 30 min followed by stimulation with CCN1, p-p65 expression was investigated by western blot. (F&G) Cells pretreated with αvβ3, αvβ5, α5β1, and α6β1 antibody for 30 min were stimulated with CCN1, p-p65 expression rated by western blot. Results are expressed as mean ± S.E. *, p<0.05 compared with control; #, p<0.05 compared with CCN1-treated group.</p

CCN1 enhances OSM expression via αvβ3 integrin in osteoblasts.

<p>(A&B) Cells were incubated with various concentrations of CCN1 (3–30 ng/ml), the OSM mRNA and protein levels rated by quantitative polymerase chain reaction (qPCR), western blot, and ELISA respectively. (C&D) Osteoblasts were incubated with CCN1 in time interval, mRNA and protein expression of OSM analyzed by qPCR, western blot, and ELISA, respectively. (E) Osteoblasts were incubated with various concentrations of CCN1 (3–30 ng/ml), the OSM luciferase activity rated by luciferase assay. (F&G) MC3T3-E1 cells were incubated with various concentrations of CCN1 (3–30 ng/ml) for 24, the OSM levels rated by western blot and ELISA. (H) Osteoblasts were incubated with CCN1 for 24 h, mRNA expression of various integrins assessed by qPCR. (I) Cells pretreated with αvβ3 for 30 min were stimulated by CCN1, OSM expression analyzed by qPCR. (J&K) Osteoblasts were pretreated with αvβ3, αvβ5, α5β1 and α6β1 integrin antibody for 30 min followed by stimulation with CCN1 for 24 h, the OSM levels rated by western blot and ELISA. Results are expressed as mean ± S.E. *, p<0.05 compared with control; #, p<0.05 compared with CCN1-treated group.</p