Media 2: Photophysical mechanisms of collagen modification by 80 MHz femtosecond laser

Originally published in Optics Express on 08 November 2010 (oe-18-23-24037

Media 1: Photophysical mechanisms of collagen modification by 80 MHz femtosecond laser

Originally published in Optics Express on 08 November 2010 (oe-18-23-24037

Media 3: Photophysical mechanisms of collagen modification by 80 MHz femtosecond laser

Originally published in Optics Express on 08 November 2010 (oe-18-23-24037

Single-shot quantitative polarization imaging of complex birefringent structure dynamics

Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a multitude of polarization light microscopy techniques are often used to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performances of PSIM are validated by imaging a rotating wave plate and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to $350\times350 \mu m^2$ and a diffraction-limit spatial resolution of $\sim 2\mu m$. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions

Visualizing Dynamics of Sub-Hepatic Distribution of Nanoparticles Using Intravital Multiphoton Fluorescence Microscopy

Nanoparticles that do not undergo renal excretion or <i>in vivo</i> degradation into biocompatible debris often accumulate in the reticuloendothelial system, also know as the mononuclear phagocyte system, with undesired consequences that limit their clinical utility. In this work, we report the first application of intravital multiphoton fluorescence microscopy to dynamically track the hepatic metabolism of nanoparticles with subcellular resolution in real time. Using fluorescently labeled mesoporous silica nanoparticles (MSNs) in mice as a prototypical model, we observed significant hepatocyte uptake of positively charged, but not negatively charged, moieties. Conversely, <i>in vivo</i> imaging of negatively charged, but not positively charged, MSNs reveals an overwhelming propensity for the former’s rapid uptake by Kupffer cells in liver sinusoids. Since the only prerequisite for these studies was that nanoparticles are fluorescently labeled and not of a specific composition or structure, the techniques we present can readily be extended to a wide variety of nanoparticle structures and surface modifications (<i>e.g.</i>, shape, charge, hydrophobicity, PEGylation) in the preclinical assessment and tailoring of their hepatotoxicities and clearances

Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics

Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350 × 350 μm2 and a diffraction-limit spatial resolution of ∼2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions

Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics

Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350 × 350 μm2 and a diffraction-limit spatial resolution of ∼2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions

Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics

Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350 × 350 μm2 and a diffraction-limit spatial resolution of ∼2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions

Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics

Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350 × 350 μm2 and a diffraction-limit spatial resolution of ∼2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions

Visualization 1: Direct visualization of functional heterogeneity in hepatobiliary metabolism using 6-CFDA as model compound

Movie Originally published in Biomedical Optics Express on 01 September 2016 (boe-7-9-3574