18 research outputs found

    The 11 selected candidate asRNAs for RT-PCR validation.

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    <p>The 11 selected candidate asRNAs for RT-PCR validation.</p

    The 9 selected candidate intergenic sRNAs for RT-PCR validation.

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    <p>The 9 selected candidate intergenic sRNAs for RT-PCR validation.</p

    Validation of intergenic sRNA expression by RT-PCR.

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    <p>Electrophoresis of the PCR amplicons of the 9 selected intergenic sRNAs using 3% agarose gels. Lane M: Marker, Lane 1-9: SYPCC_Igr_01, SYPCC_Igr_02, SYPCC_Igr_03, SYPCC_Igr_04, SYPCC_Igr_05, SYPCC_Igr_06, SYPCC_Igr_07 SYPCC_Igr_08, and SYPCC_Igr_09.</p

    Validation of 5β€²-leader region expression by RT-PCR.

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    <p>Electrophoresis of PCR amplicons of 7 5β€²-leader regions using 3% agarose gels. Lane M: Marker, Lane 1-7: SYPCC_Ir_01, SYPCC_Ir_02, SYPCC_Ir_03, SYPCC_Ir_04, SYPCC_Ir_05, SYPCC_Ir_06, and SYPCC_Ir_07.</p

    The 7 selected candidate 5β€²-leader regions for RT-PCR validation.

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    <p>The 7 selected candidate 5β€²-leader regions for RT-PCR validation.</p

    Validation of asRNA expression by RT-PCR.

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    <p>Electrophoresis of the PCR amplicons of 11 asRNAs using 3% agarose gels. Lane M: Marker, Lane 1–11: SYPCC_anti_01, SYPCC_anti_02, SYPCC_anti_03, SYPCC_anti_04, SYPCC_anti_05, SYPCC_anti_06, SYPCC_anti_07, SYPCC_anti_08, SYPCC_anti_09, SYPCC_anti_10, and SYPCC_anti_11.</p

    GO analyses of predicted target sRNA genes.

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    <p>GO analysis of target genes that are predicted to be involved in biological processes A, molecular functions B and their cellular components C. Classified target gene objects are depicted as percentages (in brackets) of the total number of gene objects with GO assignments.</p

    Pie charts classifying the deep sequencing reads.

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    <p>A, The percentages of all the deep sequencing reads mapped to the previously annotated or non-annotated regions of the <i>Synechocystis</i> sp. PCC 6803 genome. B, The relative proportions of the deep sequencing reads (n β€Š=β€Š 4,320,855) mapped to the annotated regions. C, The relative percentages of the deep sequencing reads (n β€Š=β€Š 1,807,035) mapped to the nonannotated regions. IGR, intergenic region; Antisense, reads located on the cis-antisense strand of a known gene; misc_rna, RNA of unknown function.</p

    Chl fluorescence parameters of <i>C. sorokiniana</i> C3 during OD formation.

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    <p>Chl fluorescence of cells was monitored with a Dual-PAM-100 fluorimeter. Black square symbols, cells resuspended in N+ medium. Solid up arrow symbols, cells resuspended in Nβˆ’ medium. A, Fv/Fm, B, Fvβ€²/Fmβ€², C, 1-qL, D, Y(NO). Measurements were performed in darkness at room temperature and saturation pulses were applied every 30 s. Data are shown as the means Β± SD (nβ€Š=β€Š3).</p

    The predicted target sRNA genes involved in photosynthesis.

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    <p>The predicted target sRNA genes involved in photosynthesis.</p
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