Reagents and antibodies for Tfh panel.

Reagents and antibodies for Tfh panel.</p

Racial disparities in the distribution of Tfh cells.

A) Overall Tfh frequencies were similar between African-Americans and Caucasians. However, race related differences were observed in certain Tfh cell subsets including B, C) decreased frequencies of Tfh1 cells in African-Americans at all age ranges, and D, E) increased Tfh17 frequencies in African-Americans, a difference that was observed in all age groups. In C and E, Caucasians are represented by blue bars and African-Americans by orange bars.</p

Age related changes in selected T cell populations.

A, B) A decrease in naïve CD4+ and CD8+ T cells with advancing age (C, D) corresponds with an increase in central memory CD4+ and CD8+ T cells. E) Effector memory CCR4+ Tregs increase with age. Tregs, identified by gating on CD4+CD25+CD127low cells were compared based on four age groups.</p

Example of web-based data visualization tool.

Researchers have the ability to select reference ranges for flow cytometry panels of interest by age, gender, and race filters. This tool will calculate summary statistics on the selected population and the data may be downloaded for study. Links to cell ontology are present when applicable, and the gating strategies are available. Gating strategies and summary tables are also available.</p

Study population demographics.

Study population demographics.</p

Forest plot summarizing reference ranges for major lymphocyte subsets in the overall population.

References are shown for CD3+ T cells, CD4+ T cells, CD8+ T cells, Tfh cells, overall B cells, monocytes, NK cells, Tregs, and dendritic cells. Data are presented as means with 95% reference intervals calculated by non-parametric bootstrap.</p

Reagents and antibodies for HIPC panels.

Reagents and antibodies for HIPC panels.</p

Racial disparities in the distribution of innate cells.

A) Classical (CD14+CD16-) monocytes were decreased and B) non-classical (CD16+CD14-) were increased in African-Americans compared with Caucasians. C) The increase in CD16+CD14- populations in African-Americans was present in all age groups. D) In each age group, the frequency of CD14+ monocytes was higher in Caucasians compared to African-Americans. E) CD16+CD56- NK cell frequencies were increased in African-Americans compared to Caucasians, and the difference became larger in the older age groups. In C, D, and E, Caucasians are represented by blue bars and African-Americans by orange bars.</p

Identification of cell subsets in the blood.

T, B, and innate cells were identified according to HIPC guidelines. The Tfh panel is not part of the HIPC guidelines and markers were selected by the study team based on published medical literature.</p

Table S3 from Immune Activation in Early-Stage Non–Small Cell Lung Cancer Patients Receiving Neoadjuvant Chemotherapy Plus Ipilimumab

Signed-Rank Tests of V3-V2 Difference</p