MOESM2 of Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum

Additional file 2: Fig. S2. C. thermocellum growth profile in terms of production of metabolites (ethanol + acetic acid + lactic acid) and pellet nitrogen content (g/L) as a proxy for cell growth for 24 h under growth conditions in a bottle with 200 mL working volume and 5 g/L glucan Avicel® PH-101 loading without active pH control in MOPS buffer. Measured in triplicates from one culture bottle

MOESM1 of Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum

Additional file 1: Fig. S1. Enzymatic hydrolysis (EH) glucan yield time profile on autoclaved vs. unautoclaved switchgrass with (a) 15 mg protein/g glucan and (b) 65 mg protein/g glucan enzyme loadings of Accellerase® 1500. A 0.5 wt% glucan loading with a working mass of 50 g was used for EH which was performed in triplicates with controls at 50 °C, 150 rpm

MOESM1 of Flowthrough pretreatment with very dilute acid provides insights into high lignin contribution to biomass recalcitrance

Additional file 1. Detailed material balances for Stage 1 (pretreatment) and Stage 2 (enzymatic hydrolysis) for all process conditions

MOESM1 of Natural genetic variability reduces recalcitrance in poplar

Additional file 1. Additional details for rare natural poplar variants. Additional file 1 provides the list of plants and their lignin content. This file also provides details of the graphs with box plots, and average imprecisions in measurement of sugar yield and lignin content

MOESM1 of Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes

Additional file 1. Additional information

MOESM4 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

Additional file 4. Chromatographic profiles of the sugar standards in the HPAEC method. As outlined in “Methods” section, the HPAEC analyses were carried out in two separate runs using two different programs (i.e. different columns and gradients) for each sample. In bold are the sugars quantified using the respective program. (A) Program 1 was used to quantify the amounts of fucose (Fuc), rhamnose (Rha), arabinose (Ara), galactose (Gal), glucose (Glc), galacturonic acid (GalA), and glucuronic acid (GlcA) on a Dionex PA20 column eluted using a NaOH/NaOAc gradient. (B) Program 2 was used to quantify the amounts of xylose (Xyl) and mannose (Man), which eluted as one peak in program 1, on a Dionex PA1 column eluted isocratically using 2 mM NaOH

MOESM5 of Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

Additional file 5. Comparison of the mol% of the different types of sugars in leaf AIR from Arabidopsis, Populus, rice and switchgrass using the four different glycosyl residue composition analysis methods

MOESM1 of Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems

Additional file 1: Table S1. Statistical analysis of cellulose crystallinity, lignin molecular weight and PDI, and water retention value (WRV) for raw, pretreated, and biological residues of Populus natural variants

MOESM1 of Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment

Additional file 1. Supplemental informatio

BVRI photometric observations and light-curve analysis of GEO objects

BVRI photometric observations of Geosynchronous Earth Orbit (GEO) objects were conducted with the 1.5 m Cassini Telescope located in Loiano, Italy. The observatory is operated by the INAF (National Institute for Astrophysics) Astronomical Observatory of Bologna, Italy. The Ritchey–Chre´tien optical system is equipped with the BFOSC (Bologna Faint Object Spectrograph and Camera), a multipurpose instrument for imaging and spectroscopy, with an EEV CCD (13401300 pixel). This paper deals with the results of the photometric observations of several targets from the SSN (Space Surveillance Network) catalog that were acquired in May and December 2013