Diversity, antimicrobial production, and seasonal variation of honey bee microbiota isolated from the honey stomachs of the domestic honey bee, Apis mellifera

The antimicrobial nature of honey and its related apiological origins typically focus on basic chemical analysis without attempting to understand the diversity of the microbial component. The antibacterial activity, chemical characterization, and diversity of bacteria isolated from Apis mellifera honey stomachs and hive honey collected throughout the honey production season are presented. After screening >2,000 isolates, 50 isolates were selected and characterized by 16S rRNA gene homology, Gram stain, catalase and protease tests, as well as for antibacterial activity against select indicators. Antibacterial-producing isolates were predominantly from the Pseudomonas, Paenibacillus, Lonsdalea, Serratia, and Bacillus genera. Isolates collected from honey stomachs in April displayed the highest level of activity (27%). While April isolates did not demonstrate activity against the Gram-negative bacteria tested. Whereas 59% of July isolates, 33% of September isolates, and 100% of the honey isolates did. The predominant honey stomach isolates were Pseudomonas spp. (April), Paenibacillus polymyxa (July, Sept.), and Lonsdalea iberica (Sept.). Chemical characterizations of the antimicrobial compounds show most to be antibiotic in nature with the minority being potential bacteriocins. This study offers the first glimpse into the variability and diversity of the bacteria/host interactions found within the honey stomach of the domestic honey bee while revealing a novel source of potentially beneficial antimicrobial compounds

Engaging young audiences

Introduction Science outreach plays a pivotal role in fostering understanding of scientific concepts, enhancing community engagement, and inspiring the next gen­eration of scientists. This engagement with the pub­lic is significant for land-grant universities, which were established with the mission to enhance agri­culture education and research and to ensure that knowledge is accessible to the public (Mcdowell, 2003). Included in the mission is a dedicated effort aimed at younger audiences, including elementary, middle, and high school students. Such engage­ments are valuable at stimulating early interest in science. There is evidence that engaging students with hand-on scientific exploration and inquiry enhances their understanding and fosters a positive attitude toward science (Clark et al., 2016; Ecklund et al., 2012). Career fairs, specifically, can serve as valuable platforms for such outreach, particularly with bridging the persistent gap between academia and public understanding. They can help to inform, engage, and inspire students and encourage them to explore various career paths, including agricul­ture and food science. Young people consume food without fully appreciating the intricate com­ponents of the food system, which encompasses agricultural production, harvesting, processing, dis­tribution, and retail. This system is highly intercon­nected and complex, involving multiple stakehold­ers such as farmers, processors, distributors, retailers, and consumers. . .

Curli Production Influences Cross-contamination by Escherichia coli O157:H7 When Washing Fresh-cut Romaine Lettuce

Escherichia coli O157:H7 expresses extracellular proteins called curli that are essential for surface colonization. Transfer rates of E. coli O157:H7 0018+ (curli+), and 0018- (curli−) from inoculated to noninoculated lettuce pieces during washing were quantified in this study. Romaine lettuce pieces were inoculated with ∼6 log CFU on just the surface, just the cut edges, or both surface and cut edges. Samples were dried for 2 h in a biosafety cabinet and then washed with ten (10) noninoculated lettuce pieces in 500 mL of water for 30 s. The curli− strain was more readily removed (3 log reduction) compared to the curli+ (1 log reduction) when only the lettuce surface was inoculated (p > 0.05). The same was true when only the lettuce piece edge was inoculated (p > 0.05), although the magnitude of the reduction was less. There was no significant difference in reduction of curli+ strain between any of the surfaces. There was a significant difference (p  0.05). When the leaf surface was inoculated, there was about 2 log percent (i.e., close to 100% transfer) into the wash water for both the curli+ and curli− strains. When only the cut edges or surface and edge were inoculated, observed mean transfer rates were lower but not significantly different (p > 0.05). Further research is needed to more fully understand the factors that influence bacterial cross-contamination during the washing of fresh produce

Enhancing Juice Safety and Technology Knowledge for Small Producers: A Needs Based Assessment Outreach Activities Initiative in New York State

Small- to mid-sized juice and beverage producers face unique challenges related to food safety, regulatory compliance, and technical capacity. To better understand these challenges and inform targeted outreach, a need assessment survey was conducted as part of an Extension Outreach and Assessment (EOA) project sponsored by Cornell AgriTech. The 25-question online survey, distributed via extension networks, was completed by 30 producers across New York State, representing a diverse range of businesses, production scales, and distribution strategies. Survey results revealed critical knowledge gaps in areas such as shelf-life determination, non-thermal processing, and regulatory compliance. These findings guided the development of two one-hour webinars focused on product quality and juice safety. Two webinars were conducted "Food Safety and Shelf life Strategies for Small Juice Beverage Producers" and "Safety and Quality in Juice Processing: Validating Non-thermal Processes" , reaching about 120 participants and offering high-impact learning on shelf-life, validation, and non-thermal processing. Attendance data highlighted strong engagement and interest across both sessions. The webinars incorporated live Q&A sessions to enhance engagement and assess learning outcomes. This ongoing project highlights the importance of data-driven outreach and underscores the need for continued support and training tailored to the specific needs of small-scale juice and juice-containing processors.This project was supported by Cornell AgriTech Extension Outreach Assistanshi

Survival of Foodborne Pathogens in Low and Nonalcoholic Craft Beer

Breweries and beverage companies have recently been interested in creating innovative beer varieties that deviate from traditional beer styles, with either low-alcohol content <2.5% alcohol by volume (ABV) or the absence of alcohol altogether (<0.5% ABV). Traditional beers (up to 10% ABV) contain numerous intrinsic and extrinsic factors preventing pathogens from proliferation or propagation. Physiochemical properties such as a low pH, presence of ethanol and hop acids, limited oxygen, and specific processing techniques, including wort boiling, pasteurization, filtration, cold storage, and handling, all contribute to microbial stability and safety. The potential change or absence in one or more of these antimicrobial hurdles can render the final product susceptible to pathogen survival and growth. In this study, the effect of pH, storage temperature, and ethanol concentration on the growth or die-off of foodborne pathogens in low and nonalcoholic beers was evaluated. pH and ethanol concentrations were adjusted from their initial values of 3.65 and <0.50% ABV to pHs 4.20, 4.60, and 4.80; and 3.20 ABV, respectively. The samples were inoculated with individual five-strain cocktails of E. coli O157:H7, S. enterica, and L. monocytogenes, then stored at two different temperatures (4 and 14°C) for 63 days. Microbial enumeration was performed using selective agar with incubation at 35°C. Results showed that nonalcoholic beers allowed for pathogen growth and survival, as opposed to the low-alcoholic ones. E. coli O157:H7 and S. enterica grew approximately 2.00 log  at 14°C, but no growth was observed at 4°C. L. monocytogenes was more susceptible and fell at, or below, the detection limit rapidly in all the conditions tested. The results show that storage temperature is critical in preventing the growth of pathogens. pH did not appear to have a significant effect on the survival of pathogens (p < 0.05). This challenge study demonstrates the need for beverage manufacturers to prioritize and maintain food safety plans along with practices specific to low- and nonalcoholic beer manufacturers

Diversity, antimicrobial production, and seasonal variation of honey bee microbiota isolated from the honey stomachs of the domestic honey bee, Apis mellifera

The antimicrobial nature of honey and its related apiological origins typically focus on basic chemical analysis without attempting to understand the diversity of the microbial component. The antibacterial activity, chemical characterization, and diversity of bacteria isolated from Apis mellifera honey stomachs and hive honey collected throughout the honey production season are presented. After screening &amp;gt;2,000 isolates, 50 isolates were selected and characterized by 16S rRNA gene homology, Gram stain, catalase and protease tests, as well as for antibacterial activity against select indicators. Antibacterial-producing isolates were predominantly from the Pseudomonas, Paenibacillus, Lonsdalea, Serratia, and Bacillus genera. Isolates collected from honey stomachs in April displayed the highest level of activity (27%). While April isolates did not demonstrate activity against the Gram-negative bacteria tested. Whereas 59% of July isolates, 33% of September isolates, and 100% of the honey isolates did. The predominant honey stomach isolates were Pseudomonas spp. (April), Paenibacillus polymyxa (July, Sept.), and Lonsdalea iberica (Sept.). Chemical characterizations of the antimicrobial compounds show most to be antibiotic in nature with the minority being potential bacteriocins. This study offers the first glimpse into the variability and diversity of the bacteria/host interactions found within the honey stomach of the domestic honey bee while revealing a novel source of potentially beneficial antimicrobial compounds.</jats:p