The <i>gl13</i> gene encodes a putative ABC transporter G family protein.

<p>The amino acid sequence of the 2 GL13 isoforms (GRMZM2G118243_P01 and GRMZM2G118243_P02) were aligned to 9 homologs downloaded from GenBank (Table S2). Neighbor-joining analysis was used to generate a phylogenetic tree, which was bootstrapped over 1,000 cycles, using MEGA5.0. </p

Phenotypic characterization of the <i>gl13-ref</i> mutant phenotype.

<p>(A) Comparisons of gross morphology and epicuticular wax accumulation and morphology in mutant (upper) and wild-type (lower) seedlings; (B) Comparisons of paraffin sections (10x) of leaves from mutant (left) and wild-type (right) seedlings stained with Fast Green; (C) Detached leaves from mutant (upper) and wild-type (lower) immediately after harvest and after 2 hours at room temperature; (D) Water loss from detached mutant and wild-type seedling leaves at room temperature. Error bars = SE. </p

BSR-Seq Mapping and Seq-Walking of <i>gl13</i>.

<p>(A) Numbers of informative SNPs used in BSR-Seq analyses of 3 EMS-induced <i>gl13</i> alleles. (B) Results of BSR-Seq analysis for three alleles of <i>gl13</i>. (C) Crossing strategy used to generate seedlings homozygous for wild-type and <i>gl13-Mu 90-3134B1</i> alleles for Seq-Walking. (D) Identification of shared, wild-type specific, and mutant-specific Mu-flanking sequence (MFS) sites and identification of mutant-specific MFS located with the <i>gl13</i> mapping interval. </p

Quantitative RT-PCR analysis of <i>gl13</i>.

<p>(A) Transcript accumulation from the <i>gl13</i> gene was measured via qRT-PCR in 8 EMS-induced alleles and 2 spontaneous alleles and compared to a wild-type control (left box) and the Mu-induced allele and its appropriate wild-type control (right box). (B) The relative expression of <i>gl13</i> in shoots (6 DAP) and seedlings (10 DAP and 15 DAP) from wild-type and <i>gl13-ref</i> mutant. </p

Sequence analysis of 11 <i>gl13</i> alleles.

<p>The positions of detected lesions are indicated by arrows within the <i>gl13</i> gene. Green boxes indicate gene coding region (CDS) and yellow boxes indicate un-translated region (UTR). Alleles are numbered according to Table 1. Four EMS-induced premature terminal codon (PTC) mutations are shown in red; two EMS-induced non-synonymous substitutions are shown in green; an EMS-induced G->A transition is shown in blue; the location of a <i>Mu</i> insertion (belong to Mu1 family [25]) is shown in pink; the location of a C->A transversion found in two spontaneous alleles are shown in yellow; and a 2bp deletion detected in one EMS-induced allele is shown in black. </p