18 research outputs found

    GO categories of the unigenes.

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    <p>The unigenes were annotated in three categories: biological processes, cellular components and molecular functions.</p

    Validation of candidate unigenes and miRNAs in <i>A. konjac</i> and <i>A. bulbifer</i> by qRT-PCR.

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    <p>(a) Five candidate unigenes show differential expression patterns by qRT-PCR in <i>A. konjac</i> and <i>A. bulbifer</i>. (b) Four candidate miRNAs show differential expression patterns by qRT-PCR in <i>A. konjac</i> and <i>A. bulbifer</i>.</p

    <i>De Novo</i> Transcriptome and Small RNA Analyses of Two Amorphophallus Species

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    <div><p>Konjac is one of the most important glucomannan crops worldwide. The breeding and genomic researches are largely limited by the genetic basis of <i>Amorphophallus</i>. In this study, the transcriptomes of <i>A. konjac</i> and <i>A. bulbifer</i> were constructed using a high-throughput Illumina sequencing platform. All 108,651 unigenes with average lengths of 430 nt in A. konjac and 119,678 unigenes with average lengths of 439 nt were generated from 54,986,020 reads and 52,334,098 reads after filtering and assembly, respectively. A total of 54,453 transcripts in <i>A. konjac</i> and 55,525 in <i>A. bulbifier</i> were annotated by comparison with Nr, Swiss-Prot, KEGG, and COG databases after removing exogenous contaminated sequences. A total of 80,332 transcripts differentially expressed between <i>A. konjac</i> and <i>A. bulbifer.</i> The majority of the genes that are associated with konjac glucomannan biosynthetic pathway were identified. Besides, the small RNAs in <i>A. konjac</i> leaves were also obtained by deep sequencing technology. All of 5,499,903 sequences of small RNAs were obtained with the length range between 18 and 30 nt. The potential targets for the miRNAs were also predicted according to the konjac transcripts. Our study provides a systematic overview of the konjac glucomannan biosynthesis genes that are involved in konjac leaves and should facilitate further understanding of the crucial roles of carbohydrate synthesis and other important metabolism pathways in <i>Amorphophallus</i>.</p></div

    Proposed pathways of konjac glucomannan biosynthesis.

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    <p>The identified enzymes in <i>Amorphophallus</i> are noted in blue and the unidentified is in red. Sucrose synthase (SuS), invertase (INV), phosphoglucose isomerase (PGI), phosphoglucomutase (PGM), phosphomannose isomerase (PMI), phosphomannomutase (PMM), starch synthase (SS), GDP-mannose pyrophosphorylase (GMPP), UDP-glucose pyrophosphorylase (UGP), ADP-glucose pyrophosphorylase (AGP), fructokinase (FRK), hexokinase (HXK), starch branching enzyme (SDB), cellulose synthase-like A (CSLA), Cellulose synthase-like D (CSLD), GDP-D-pyrophosphorylase (GGP)</p
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