8 research outputs found

    Transcriptome Profiling Reveals Th17-Like Immune Responses Induced in Zebrafish Bath-Vaccinated with a Live Attenuated <i>Vibrio anguillarum</i>

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    <div><p>Background</p><p>A candidate vaccine, live attenuated <i>Vibrio anguillarum</i> developed in our laboratory could prevent vibriosis of fish resulted from <i>V. anguillarum</i> and <i>V. alginolyticus</i>. To elucidate the molecular mechanisms underlying the vaccine protection, we used microarray technology to compare the spleen transcriptomes of bath-vaccinated and unvaccinated zebrafish at 28 days post vaccination.</p><p>Principal Findings</p><p>A total of 2164 genes and transcripts were differentially expressed, accounting for 4.9% of all genes represented on the chip. In addition to iron metabolism related to the innate immunity and the signaling pathways, these differentially expressed genes also involved in the adaptive immunity, mainly including the genes associated with B and T cells activation, proliferation and expansion. Transcription profiles of Th17-related transcription factors, cytokines and cytokine receptors during 35 days post-vaccination implied that Th17 cells be activated in bath-vaccinated zebrafish.</p><p>Conclusion/Significance</p><p>The transcriptome profiling with microarray revealed the Th17-like immune response to bath-vaccination with the live attenuated <i>V. anguillarum</i> in zebrafish.</p></div

    Effects of vaccination with the live attenuated <i>V. anguillarum</i> on Wnt signaling pathway.

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    <p>The genes related to Wnt signaling pathway were modulated after vaccination with the live attenuated <i>V. anguillarum</i>. Red: up-regulated, Green: down-regulated, Black: not found to be modulated. WNT: wingless-type MMTV integration site family, DKK1: Dickkopf homologue 1, LRP5/6: LDL-receptor-related protein 5/6, CK1: casein kinase, GSK3β: glycogen synthase kinase 3β, AXIN: axis inhibition protein, DVL: mammalian homologue of <i>Drosophila</i> dishevelled, APC : adenomatous polyposis coli, PKA: cAMP-dependent protein kinase catalytic subunit alpha, PS-1: presenilin 1, PYGO: legless CREB binding protein, LGS: pygopus CREB binding protein, TCF: T-cell factor, GRG:Groucho, CTBP: C-terminal binding protein, HDAC: histone deacetylases, STBM: VANGL planar cell polarity protein 2, DAAM: dishevelled associated activator of morphogenesis, RHOA: ras homolog family member A, RAC: ras-related C3 botulinum toxin substrate, ROCK: Rho-associated coiled-coil containing protein kinase, JNK: Jun N-terminal kinase, JUN: jun proto-oncogene, PLC: phospholipase C, PKC: protein kinase C, CAMKII: calcium calmodulin mediated kinase II, NFAT: nuclear factor of activated T cells.</p

    Iron uptake and metabolism were enhanced in the bath-vaccinated zebrafish.

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    <p>Genes related to putative iron metabolism of intracellular iron and competition for extracellular iron were differentially modulated after vaccination with the live attenuated <i>V. anguillarum</i>. Red: up-regulated, Green: down-regulated, Black: not found to be modulated.</p

    Validation of relative expression between microarray data and RT-qPCR results at 28 days post vaccination.

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    <p>iNOS2a: inducible nitric oxide synthase 2, itga3b: integrin alpha 3b, STAT5: Signal Transducer and Activator of Transcription 5, IL7R: interleukin 7 receptor, IL22: interleukine 22, Bcl6ab: B-cell CLL/lymphoma 6a, Cdc42l: cell division cycle 42 like, Mycb: myelocytomatosis oncogene b, ABCB8: ATP-binding cassette sub-family B member 8, Cts1a: cathepsin L 1 a.</p

    GO function annotation results of differentially expressed genes.

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    *<p>the percentage of genes in the specific subcategory from each of the three GO ontologies.</p
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