48 research outputs found
Lysozyme Amyloid Fibril-Integrated PEG Injectable Hydrogel Adhesive with Improved Antiswelling and Antibacterial Capabilities
Hydrogels
with inherent antibacterial activities have been attracting
increasing attention, particularly for biomedical applications. Biology
provides a range of materials and mechanisms to meet diverse requirements
for bacterial combating. Lysozyme after fibrillation (LZMF) has a
much superior antibacterial ability than globular native lysozyme
due to its decreased positive charges and increased hydrophobic β-sheet
component. Here, we propose to design a polyÂ(ethylene glycol) (PEG)
cross-linked LZMF composite antibacterial hydrogel by utilizing the
nucleophilic substitution reaction between LZMF and N-hydroxysuccinimide end groups on four-arm PEG-NHS. The generated
PEG-LZMF hydrogel is bacteria-resistant both in vitro and in vivo as expected and has good biocompatibility.
Moreover, the volume expansion of PEG can be significantly inhibited
due to the presence of hydrophobic lysozyme amyloid fibrils. In addition,
the relatively fast cross-linking reaction can make PEG-LZMF both
injectable and shape-compatible. The simultaneous reaction with tissue-exposed
−NH2 or −SH also confers a tissue-adhesive
ability. We envision that this hydrophobic lysozyme amyloid fibril-integrated
PEG composite hydrogel can effectively adhere/protect open wounds
and internal incisions and suppress pathogen infection through a biomimetic
antibacterial mechanism. Considering the simple fabrication process,
this multifunctional PEG-LZMF antibacterial hydrogel is promising
for clinical transformation
Additional file 1 of Construction of fatty acid derivatives from rubber seed oil as α-glucosidase inhibitors based on rubber seed oil
Additional file 1. Supporting information
Lysozyme Amyloid Fibril-Integrated PEG Injectable Hydrogel Adhesive with Improved Antiswelling and Antibacterial Capabilities
Hydrogels
with inherent antibacterial activities have been attracting
increasing attention, particularly for biomedical applications. Biology
provides a range of materials and mechanisms to meet diverse requirements
for bacterial combating. Lysozyme after fibrillation (LZMF) has a
much superior antibacterial ability than globular native lysozyme
due to its decreased positive charges and increased hydrophobic β-sheet
component. Here, we propose to design a polyÂ(ethylene glycol) (PEG)
cross-linked LZMF composite antibacterial hydrogel by utilizing the
nucleophilic substitution reaction between LZMF and N-hydroxysuccinimide end groups on four-arm PEG-NHS. The generated
PEG-LZMF hydrogel is bacteria-resistant both in vitro and in vivo as expected and has good biocompatibility.
Moreover, the volume expansion of PEG can be significantly inhibited
due to the presence of hydrophobic lysozyme amyloid fibrils. In addition,
the relatively fast cross-linking reaction can make PEG-LZMF both
injectable and shape-compatible. The simultaneous reaction with tissue-exposed
−NH2 or −SH also confers a tissue-adhesive
ability. We envision that this hydrophobic lysozyme amyloid fibril-integrated
PEG composite hydrogel can effectively adhere/protect open wounds
and internal incisions and suppress pathogen infection through a biomimetic
antibacterial mechanism. Considering the simple fabrication process,
this multifunctional PEG-LZMF antibacterial hydrogel is promising
for clinical transformation
Data_Sheet_1_A Radiomics Approach to Assess High Risk Carotid Plaques: A Non-invasive Imaging Biomarker, Retrospective Study.zip
ObjectiveThis study aimed to construct a radiomics-based MRI sequence from high-resolution magnetic resonance imaging (HRMRI), combined with clinical high-risk factors for non-invasive differentiation of the plaque of symptomatic patients from asyptomatic patients.MethodsA total of 115 patients were retrospectively recruited. HRMRI was performed, and patients were diagnosed with symptomatic plaques (SPs) and asymptomatic plaques (ASPs). Patients were randomly divided into training and test groups in the ratio of 7:3. T2WI was used for segmentation and extraction of the texture features. Max-Relevance and Min-Redundancy (mRMR) and least absolute shrinkage and selection operator (LASSO) were employed for the optimized model. Radscore was applied to construct a diagnostic model considering the T2WI texture features and patient demography to assess the power in differentiating SPs and ASPs.ResultsSPs and ASPs were seen in 75 and 40 patients, respectively. Thirty texture features were selected by mRMR, and LASSO identified a radscore of 16 radiomics features as being related to plaque vulnerability. The radscore, consisting of eight texture features, showed a better diagnostic performance than clinical information, both in the training (area under the curve [AUC], 0.923 vs. 0.713) and test groups (AUC, 0.989 vs. 0.735). The combination model of texture and clinical information had the best performance in assessing lesion vulnerability in both the training (AUC, 0.926) and test groups (AUC, 0.898).ConclusionThis study demonstrated that HRMRI texture features provide incremental value for carotid atherosclerotic risk assessment.</p
Bioinspired, Injectable, Quaternized Hydroxyethyl Cellulose Composite Hydrogel Coordinated by Mesocellular Silica Foam for Rapid, Noncompressible Hemostasis and Wound Healing
Massive bleeding
control and anti-infection are the major challenges for urgent trauma
with deep and noncompressible hemorrhage in both clinic and battlefield.
Inspired by the coordinated primarily blood clot formation and secondly
coagulation cascade activation in natural hemostasis process, an injectable,
quaternized hydroxyethyl cellulose/mesocellular silica foam (MCF)
hydrogel sponge (QHM) for both hemorrhage control and antibacterial
activities were prepared via one-pot radical graft copolymerization.
The as-prepared QHMs exhibited instant water-triggered expansion and
superabsorbent capacity and thereby effectively facilitated blood
components concentration. Moreover, the QHM1 with appropriate amount
of MCF (9.82 w/w %) could further activate the coagulation factors.
Synergistically, the QHM1 could reduce the plasma clotting time to
59 ± 4% in vitro and showed less blood loss than commercially
available hemostatics in vivo noncompressible hemorrhage models of
lethal rabbit-liver defect. Furthermore, the QHM with a quaternary
ammonium groups density of 2.732 mmol/g exhibited remarkable antibacterial
activities and excellent cytocompatibility. With the efficient hemostasis
efficacy and excellent antibacterial behavior, QHM dramatically facilitated
the wound healing in a full-thickness skin defect model in vivo. Thus,
this QHM represents a promising hemostatic in more widespread clinical
application
Additional file 1 of Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis
Additional file 1. Supporting Information
Core/Shell PEGS/HA Hybrid Nanoparticle Via Micelle-Coordinated Mineralization for Tumor-Specific Therapy
Nanomicelles,
by virtue of their prominent biocompatibility, degradability,
and ability to solubilize hydrophobic drugs, have been widely used
as the most effective delivery platform for anticancer drugs. However,
undesirable drug-loading capacity, unfeasible modification, poor in
vivo stability, and intratumoral penetration remain to be addressed.
Herein, we introduce a novel core/shell PEGylated polyÂ(glycerol sebacate)
(PEGS)/hydroxyapatite (HA) hybrid nanomicelle based on a unique triblock
PEGS substrate with functional carboxyls in terminals and free hydroxyls
as pendant groups. The hydrophobic doxorubicin (DOX) can be controllably
encapsulated in the core of nanomicelles via hydrogen bonding, and
ensuing in situ mineralization of HA occurs as a shell layer with
the electrostatic effect between the carboxylate radical (COO–) and calcium ion (Ca2+). Through optimizing
the coordination of PEGS nanomicelles and HA mineralization, 20–30
nm spherical nanoparticles can be formed with considerable drug loading
(0.38 mg DOX/1 mg nanoparticles) and a sensitive pH-responsive release
(about 50% release amount at pH 5.6 while <5% release amount at
pH 7.4 in 24 h). In further in vitro studies, this PEGS/HA hybrid
nanoparticle system exhibits excellent selective tumor inhibitory
efficacy, while in in vivo studies, high efficacy of tumor suppression
and low incidence of toxicity can be evidenced in a DOX-loaded PEGS/HA
group (71.7% decrease in average tumor volume compared to a control
group after 15 day hypodermic treatment). The core/shell PEGS/HA nanoparticle
coordinated with PEGS nanomicelles and in situ HA mineralization represents
high drug-loading capacity, multifunctional possibility, and tumor-selective
and responsive release profiles and could offer a highly promising
platform for tumor therapy in clinical application
Synthesis and Biological Activity of d- and l-<i>c</i><i>hiro</i>-Inositol 2,3,4,5-Tetrakisphosphate:  Design of a Novel and Potent Inhibitor of Ins(3,4,5,6)P<sub>4</sub> 1-Kinase/Ins(1,3,4)P<sub>3</sub> 5/6-Kinase
The synthesis of a novel and potent Ins(3,4,5,6)P4 1-kinase/Ins(1,3,4)P3 5/6 kinase inhibitor
and its enantiomer is described. d-chiro-Inositol 2,3,4,5-tetrakisphosphate [d-chiro-Ins(2,3,4,5)P4, 3, Figure ] and l-chiro-inositol 2,3,4,5-tetrakisphosphate [l-chiro-Ins(2,3,4,5)P4, ent-3] were
synthesized from d-1,6-di-O-benzyl-chiro-inositol and l-1,6-di-O-benzyl-chiro-inositol, respectively. We examined inhibition of the multifunctional Ins(3,4,5,6)P4 1-kinase/Ins(1,3,4)P3 5/6-kinase from bovine aorta by 3 and ent-3. Compound 3 was a potent inhibitor with an IC50 of
1.5 μM, and ent-3 was more than 20-fold less active. The results are compared to those for
other inhibitory inositol polyphosphates with structure−activity relationship discussion.
Compound 3 is a useful lead for development of further inhibitors of this important enzyme,
and ent-3 should find applications in the newly emerging Ins(1,4,5,6)P4 signaling pathway
Enhanced Cell Osteogenesis and Osteoimmunology Regulated by Piezoelectric Biomaterials with Controllable Surface Potential and Charges
Bone regeneration is a well-orchestrated
process involving
electrical,
biochemical, and mechanical multiple physiological cues. Electrical
signals play a vital role in the process of bone repair. The endogenous
potential will spontaneously form on defect sites, guide the cell
behaviors, and mediate bone healing when the bone fracture occurs.
However, the mechanism on how the surface charges of implant potentially
guides osteogenesis and osteoimmunology has not been clearly revealed
yet. In this study, piezoelectric BaTiO3/β-TCP (BTCP)
ceramics are prepared by two-step sintering, and different surface
charges are established by polarization. In addition, the cell osteogenesis
and osteoimmunology of BMSCs and RAW264.7 on different surface charges
were explored. The results showed that the piezoelectric constant
d33 of BTCP was controllable by adjusting the sintering
temperature and rate. The polarized BTCP with a negative surface charge
(BTCP−) promoted protein adsorption and BMSC extracellular
Ca2+ influx. The attachment, spreading, migration, and
osteogenic differentiation of BMSCs were enhanced on BTCP–.
Additionally, the polarized BTCP ceramics with a positive surface
charge (BTCP+) significantly inhibited M1 polarization of macrophages,
affecting the expression of the M1 marker in macrophages and changing
secretion of proinflammatory cytokines. It in turn enhanced osteogenic
differentiation of BMSCs, suggesting that positive surface charges
could modulate the bone immunoregulatory properties and shift the
immune microenvironment to one that favored osteogenesis. The result
provides an alternative method of synergistically modulating cellular
immunity and the osteogenesis function and enhancing the bone regeneration
by fabricating piezoelectric biomaterials with electrical signals