7 research outputs found

    Loss-of-function study.

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    <p>New bone formation was significantly reduced at the fracture sites of the anti–SDF-1 antibody- and AMD3100-treated mice in comparison with the specimens treated with PBS on days 14 and 7, respectively. Stained with hematoxylin and eosin.</p

    X-ray radiographs of fractured femur in fracture and TBI/fracture groups.

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    <p>At 14 days after fracture, the fracture line was visible in all groups. The callus became dense and callus formation at the fracture site in TBI/fracture group occurred more rapidly than those of the fracture group, whereas callus formation was minimal by the inhibition of SDF-1.</p

    Time course of <i>CXCR4</i> mRNA expression in TBI/fracture and fracture only models, as analyzed by real-time quantitative polymerase chain reaction (PCR) (n = 4 at each time point).

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    <p>CXCR4 mRNA expression increased by 4.8-fold on day 1 in the TBI/fracture group compared with that in the fracture group (<i>P</i><0.001), and further increased by 6-fold on day 2 (<i>P</i><0.001) and nearly 1.5-fold on day 3 (<i>P</i><0.001). Expression levels are the fold index versus the day 1 level in TBI/fracture only model. Values are the mean and SD results from triplicate real-time PCR analyses (*P<0.05; **P<0.001).</p

    Immunohistochemical staining for SDF-1 in groups TBI/fracture and fracture only on day 2 after surgery.

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    <p>The expression of SDF-1 was observed in both groups. High expression of SDF-1 protein was detected at the surrounding tissue of the damaged bone in the TBI/fracture group.</p

    In vitro chemotaxis assay.

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    <p>10 ng/ml rMuSDF-1 induced the migration of mouse BMSCs by 96%, and 100 ng/ml rMuSDF-1 by 254%. The effect was reduced after treated with AMD3100. Values are the mean and SD results from 4 independent experiments (*P<0.05; **P<0.001).</p

    The expression levels of CXCR4 protein were analysed by Western blot.

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    <p>The band of CXCR4 protein was obviously weaker in cells at passage 3 than those of cells at 21passages in culture.</p