sj-docx-1-ijj-10.1177_03064190231203692 - Supplemental material for Pattern recognition as a learning strategy in the study of engineering dynamics

Supplemental material, sj-docx-1-ijj-10.1177_03064190231203692 for Pattern recognition as a learning strategy in the study of engineering dynamics by Simon Li, Kashif Raza, Ahmad Ghasemloonia and Catherine Chua in International Journal of Mechanical Engineering Education</p

Association of epidemiological factors with clustered strains.

<p>Association of epidemiological factors with clustered strains.</p

Maximum Likelihood phylogenetic tree of 1518 global genomes and 34 concatenated genomes of pH1N1/09 viruses.

NUS sequences are in circles (red represent clusters and yellow others) and non-NUS Singaporean sequences are in blue triangles. Clusters were identified with strong bootstrap support (>70%). Trees were generated in RAxML using the GTR substitution matrix and GAMMA model of rate heterogeneity with 1000 bootstrap replicates. The best scoring tree was visualized in MEGA 6.</p

Maximum Likelihood phylogenetic tree of 34 concatenated genomes of pH1N1/09 viruses from NUS campus.

<p>Clade 6 viruses are marked with asterisk. Strain name is followed by residence status and week of isolation. On-campus sequences are in red font and Off-campus sequences are in black font. Clusters were identified with strong bootstrap support (>70%). Clusters with exclusively On-campus sequences are highlighted in grey color.</p

Phylogenetic trees of Seasonal influenza viruses circulating in a university cohort in Singapore, 2007.

<p>The phylogenetic trees of (A) 6 HA and (B) 6 (NA) genes of A/H3N2 viruses; (C) 5 HA and (D) 5 NA genes of A/H1N1 viruses with WHO vaccine strains and other 2007 sequences from GenBank constructed using neighbor-joining method. Bootstrap values 90 and over are shown. Singapore isolates are in bold and vaccine strains in rectangles.</p

Results of phylogeny trait association for pH1N1/09 viruses.

<p>Results of phylogeny trait association for pH1N1/09 viruses.</p

Influenza A Neuraminidase (NA) protein sequence Analysis for Oseltamivir Resistance.

<p>(A) The consensus sequence of neuraminidase (N1) gene from residues 251 to 300. Substitution of amino acid Histidine (H) to Tyrosine (Y) at position 275 in N1 gene (shown in bold) and at position 274 in N2 gene confers resistance to oseltamivir. Protein sequences of (B) N1 and (C) N2 genes of Singapore isolates.</p

Primer sets for sequencing HA and NA genes.

<p>*R =  A/G, Y =  C/T, M =  A/C, N =  A/C/G/T.</p

Number (%) of samples positive for influenza A virus infection by RT-PCR and viral isolation (shown in bold).

1<p><b>Present</b> means sample was positive for influenza A infection by either or both the methods.</p>2<p><b>Absent</b> means sample was negative for influenza A infection by both methods.</p

Monthly distribution of total number of samples and influenza A positive samples detected by RT-PCR and viral isolation during the study period.

<p>Monthly distribution of total number of samples and influenza A positive samples detected by RT-PCR and viral isolation during the study period.</p