Design e processo expositivo : criação e comunicação de ambientes expositivos

A necessidade de uma comunicação adequada é essencial no que concerne à transmissão e fruição de uma exposição. O Design possui as ferramentas teóricas e práticas necessárias ao cumprimento desse pressuposto. Com esta investigação, pretendemos destacar a necessidade de uma nova abordagem teórica e programática sobre o processo expositivo, ressaltando a importância dos aspectos tridimensionais e as soluções contextualizantes, que contribuam para o entendimento da exposição como veículo de comunicação. Chamamos especial atenção para o potencial de interlocução do designer de interiores como projectista de ambientes expositivos, tendo em vista a maximização da percepção do visitante. Esta interlocução pode realizar-se através do processo expositivo, sob a forma de histórias organizadas e reduzidas a ícones de significado universal, que são aplicados num espaço real. Aponta-se para a necessidade do desenvolvimento de uma teorização acerca do Design Expositivo, como instância de fundamentação para a teoria comunicacional. Com esta investigação, propõe-se ainda a análise de uma estratégia de reconhecimento da mensagem das exposições, através dos espaços e dos objectos.Abstract: The need for proper communication is essential with regard to transmission and enjoyment of a show. Design has theoretical and practical tools necessary to comply with this assumption. With this research, we intend to highlight the need for a new theoretical and programmatic approach about the exhibition process, highlighting the importance of three-dimensional aspects and contextualizing solutions that contribute to understanding the exhibition as a communication vehicle. We call special attention for the potential of dialogue of the interior designer as an exhibition designer with a view to maximizing the perception of the visitor. This dialogue can take place through the exhibition process, in form of organized stories and reduced to icons of universal significance, which are applied in real space. They point the need to develop a theory about Exhibition Design, as an instance of reasoning for theory communication. This research aims to analyse the strategy of message acknowledge of exhibitions through the spaces and objects

Admissão de Doentes com Cancro do Pulmão nas Unidades de Cuidados Intensivos

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Comparative genomics of wild type yeast strains unveils important genome diversity

<p>Abstract</p> <p>Background</p> <p>Genome variability generates phenotypic heterogeneity and is of relevance for adaptation to environmental change, but the extent of such variability in natural populations is still poorly understood. For example, selected <it>Saccharomyces cerevisiae </it>strains are variable at the ploidy level, have gene amplifications, changes in chromosome copy number, and gross chromosomal rearrangements. This suggests that genome plasticity provides important genetic diversity upon which natural selection mechanisms can operate.</p> <p>Results</p> <p>In this study, we have used wild-type <it>S. cerevisiae </it>(yeast) strains to investigate genome variation in natural and artificial environments. We have used comparative genome hybridization on array (aCGH) to characterize the genome variability of 16 yeast strains, of laboratory and commercial origin, isolated from vineyards and wine cellars, and from opportunistic human infections. Interestingly, sub-telomeric instability was associated with the clinical phenotype, while Ty element insertion regions determined genomic differences of natural wine fermentation strains. Copy number depletion of <it>ASP3 </it>and <it>YRF1 </it>genes was found in all wild-type strains. Other gene families involved in transmembrane transport, sugar and alcohol metabolism or drug resistance had copy number changes, which also distinguished wine from clinical isolates.</p> <p>Conclusion</p> <p>We have isolated and genotyped more than 1000 yeast strains from natural environments and carried out an aCGH analysis of 16 strains representative of distinct genotype clusters. Important genomic variability was identified between these strains, in particular in sub-telomeric regions and in Ty-element insertion sites, suggesting that this type of genome variability is the main source of genetic diversity in natural populations of yeast. The data highlights the usefulness of yeast as a model system to unravel intraspecific natural genome diversity and to elucidate how natural selection shapes the yeast genome.</p

Parameter estimation in spatially extended systems: The Karhunen-Loeve and Galerkin multiple shooting approach

Parameter estimation for spatiotemporal dynamics for coupled map lattices and continuous time domain systems is shown using a combination of multiple shooting, Karhunen-Loeve decomposition and Galerkin's projection methodologies. The resulting advantages in estimating parameters have been studied and discussed for chaotic and turbulent dynamics using small amounts of data from subsystems, availability of only scalar and noisy time series data, effects of space-time parameter variations, and in the presence of multiple time-scales.Comment: 11 pages, 5 figures, 4 Tables Corresponding Author - V. Ravi Kumar, e-mail address: [email protected]

The genome and transcriptome of Trichormus sp NMC-1: insights into adaptation to extreme environments on the Qinghai-Tibet Plateau

The Qinghai-Tibet Plateau (QTP) has the highest biodiversity for an extreme environment worldwide, and provides an ideal natural laboratory to study adaptive evolution. In this study, we generated a draft genome sequence of cyanobacteria Trichormus sp. NMC-1 in the QTP and performed whole transcriptome sequencing under low temperature to investigate the genetic mechanism by which T. sp. NMC-1 adapted to the specific environment. Its genome sequence was 5.9 Mb with a G+C content of 39.2% and encompassed a total of 5362 CDS. A phylogenomic tree indicated that this strain belongs to the Trichormus and Anabaena cluster. Genome comparison between T. sp. NMC-1 and six relatives showed that functionally unknown genes occupied a much higher proportion (28.12%) of the T. sp. NMC-1 genome. In addition, functions of specific, significant positively selected, expanded orthogroups, and differentially expressed genes involved in signal transduction, cell wall/membrane biogenesis, secondary metabolite biosynthesis, and energy production and conversion were analyzed to elucidate specific adaptation traits. Further analyses showed that the CheY-like genes, extracellular polysaccharide and mycosporine-like amino acids might play major roles in adaptation to harsh environments. Our findings indicate that sophisticated genetic mechanisms are involved in cyanobacterial adaptation to the extreme environment of the QTP

A Genetic Code Alteration Is a Phenotype Diversity Generator in the Human Pathogen Candida albicans

BACKGROUND: The discovery of genetic code alterations and expansions in both prokaryotes and eukaryotes abolished the hypothesis of a frozen and universal genetic code and exposed unanticipated flexibility in codon and amino acid assignments. It is now clear that codon identity alterations involve sense and non-sense codons and can occur in organisms with complex genomes and proteomes. However, the biological functions, the molecular mechanisms of evolution and the diversity of genetic code alterations remain largely unknown. In various species of the genus Candida, the leucine CUG codon is decoded as serine by a unique serine tRNA that contains a leucine 5'-CAG-3'anticodon (tRNA(CAG)(Ser)). We are using this codon identity redefinition as a model system to elucidate the evolution of genetic code alterations. METHODOLOGY/PRINCIPAL FINDINGS: We have reconstructed the early stages of the Candida genetic code alteration by engineering tRNAs that partially reverted the identity of serine CUG codons back to their standard leucine meaning. Such genetic code manipulation had profound cellular consequences as it exposed important morphological variation, altered gene expression, re-arranged the karyotype, increased cell-cell adhesion and secretion of hydrolytic enzymes. CONCLUSION/SIGNIFICANCE: Our study provides the first experimental evidence for an important role of genetic code alterations as generators of phenotypic diversity of high selective potential and supports the hypothesis that they speed up evolution of new phenotypes

Co-expression of E- and P-cadherin in breast cancer: role as an invasion suppressor or as an invasion promoter?

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Low level genome mistranslations deregulate the transcriptome and translatome and generate proteotoxic stress in yeast

Organisms use highly accurate molecular processes to transcribe their genes and a variety of mRNA quality control and ribosome proofreading mechanisms to maintain intact the fidelity of genetic information flow. Despite this, low level gene translational errors induced by mutations and environmental factors cause neurodegeneration and premature death in mice and mitochondrial disorders in humans. Paradoxically, such errors can generate advantageous phenotypic diversity in fungi and bacteria through poorly understood molecular processes.publishe