5 research outputs found

    Secondary structures of JD and GST as detected by FTIR spectroscopy.

    No full text
    <p>FTIR absorption spectra, in attenuated total reflection mode (ATR), of JD at t<sub>0</sub> h in solution, on mica and on gold are shown in the Amide I region (A). Second derivative spectra of JD at t<sub>0</sub> (B) and at t<sub>48</sub> (C) in solution, on mica and on gold are reported after normalization at the ∼1515 cm<sup>−1</sup> Tyr peak. FTIR/ATR absorption spectra of GST at t<sub>0</sub> in solution, on mica and on gold are shown in the Amide I region (D). Second derivative spectra of GST at t<sub>0</sub> (E) and at t<sub>48</sub> (F) in solution, on mica and on gold are reported after normalization at the ∼1515 cm-1 Tyr peak. The band assignment of the main peaks to the protein secondary structures are indicated.</p

    Percentage of residues organized in α-helix (black), coil (medium grey) and β-sheet (light grey) secondary structures.

    No full text
    <p>When JD is in contact with gold shows the highest value of β-sheet content with respect to JD in solution and in contact with mica (A). The number of residues arranged in α-helices (black), coils (medium grey) and β-sheets (light grey) as a function of time is reported for the JD in solution (B) on mica (C) and on gold (D).</p

    Structure of JD in physiological-like solvated environment (A) in contact with hydrophilic mica surface (B) and hydrophobic gold surface (C).

    No full text
    <p>Gold and mica are represented in grey. JD secondary structure is visualized by arrows for β-sheet structures, helices for α-helix structures and tubes for coils and turns. Specific residues are enlightened with VDW representation: the surface binding site, consisting of Arg 101 and Arg 103 residues, and the solvent-exposed aromatic patch forming a binding site specific for ubiquitin-like proteins consisting of Val86 and Trp87 spatially close to Tyr27 and Phe27.</p

    Chemical Bonds and Charge-Transfer Dynamics of a Dye–Hierarchical-TiO<sub>2</sub> Hybrid Interface

    No full text
    The adsorption of Zn-tetraphenylporphyrin (ZnTPP) on nanoporous hierarchically organized anatase TiO<sub>2</sub> structures and the properties of the corresponding hybrid interface were studied by synchrotron radiation experiments. The molecular structure, electronic properties, and bonding with nanostructured TiO<sub>2</sub> surfaces were analyzed by photoemission (XPS and UPS) and X-ray absorption spectroscopy (XAS). The charge transfer at the interface was investigated by means of valence band resonant photoemission experiments (ResPES) at the C K-edge. We show that the charge-transfer dynamics between the photoexcited ZnTPP and TiO<sub>2</sub> is strongly influenced by the presence of defects on the TiO<sub>2</sub> surface. On a stoichiometric anatase nanostructure, ZnTPP bonding occurs primarily via carbon atoms belonging to the molecular phenyl rings, and this creates a preferential channel for the charge transfer. This phenomenon is reduced in the case of defective TiO<sub>2</sub> surface, where ZnTPP interacts mainly through the molecule macrocycle. Our results represent a surface science study of the dye molecule behavior on a nanoporous TiO<sub>2</sub> photoanode relevant to dye-sensitized or hybrid solar cell applications, and they show the importance of the surface oxidation state for the charge-transfer process
    corecore