1 research outputs found
A Colloidal Stability Assay Suitable for High-Throughput Screening
A library of 32 polystyrene copolymer
latexes, with diameters ranging
between 53 and 387 nm, was used to develop and demonstrate a high-throughput
assay using a 96-well microplate platform to measure critical coagulation
concentrations, a measure of colloidal stability. The most robust
assay involved an automated centrifugation–decantation step
to remove latex aggregates before absorbance measurements, eliminating
aggregate interference with optical measurements made through the
base of the multiwell plates. For smaller nanoparticles (diameter <150
nm), the centrifugation–decantation step was not required as
the interference was less than with larger particles. Parallel measurements
with a ChemiDoc MP plate scanner gave indications of aggregation;
however, the results were less sensitive than the absorbance measurements