69 research outputs found

    Steps for microencapsulation of animal cells into hybrid alginate-silica beads.

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    <p>Cells in suspension are pre-encapsulated in Ca-alginate capsules and transferred in a diluted sodium silicate solution. The beads are finally coated with a Ca-alginate layer.</p

    In vitro determination of a proinflammatory response due to alginate or silica around cells.

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    <p>Proinflammatory response is highlighted by IL-8, which is secreted by monocytes THP-1 (immune defenders) when they are activated. The control (a) are made without THP-1 to underline the basal concentration of IL-8 produced by HepG2 cells. Results are presented as means ± 1 S.D. (n = 3).</p

    Conditions tested to perform the <i>in vitro</i> immune response test. Determination of IL-8 released in the supernatant, synonym of a proinflammatory response.

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    <p>Conditions tested to perform the <i>in vitro</i> immune response test. Determination of IL-8 released in the supernatant, synonym of a proinflammatory response.</p

    Microscopy images of an alginate capsule and a mineralized bead showing the silification.

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    <p>Brightfield (a, c, e) and fluorescent microscopy images (b, d, f) of an alginate capsule (a–b) and a mineralised bead (c–f) in the presence of PDMPO (silica tracer). Micrographs (a–d) and (e–f) correspond to representative cross sections of the materials and to a whole bead, respectively.</p

    Concentration-dependent effects of different sol-gel by-products on HepG2 cells after 5 hours of incubation.

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    <p>The membrane damaging effect of chemicals was quantified by measuring the release of the cytosolic enzyme LDH. Results are presented in percentage of cytotoxicity as means ± 1 S.D. (n = 3).</p

    Amount of human albumin secreted by HepG2 cells in the supernatant of the mineralised beads.

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    <p>Concentrations showed the daily albumin released outside of the hybrid alginate-silica material as means ± 1 S.D. (n = 3).</p

    Study of the diffusion of fluorescent probes through 7 days old mineralised beads.

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    <p>100% corresponds to the fluorescence intensity of the solution before the addition of the beads. The MWCO is estimated thanks to the hydrodynamic radius of biopolymer: 2, 6 and 11 nm for the dextran-FITC, and with latex beads carboxylated-modified characterized by a diameter of 30 nm.</p

    Microscopy images highlighting the survival of cells 6 weeks after immobilisation.

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    <p>Brightfield (a, c) and fluorescent microscopy images (b, d) of HepG2 cells three-dimensionally entrapped within a mineralised bead and labelled with fluorescein diacetate (viability probe). The micrographs were taken 1 day (a, b) and 42 days (c, d) post-encapsulation.</p

    Nitrogen adsorption-desorption isotherms.

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    <p>Isotherms obtained by analysis of purified alginate capsules (a) and mineralised beads (b).</p
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