Comparison of total dose of Gn.

<p>The total dose of Gn was significantly higher in the long protocol group than in the short protocol group for all age ranges (<i>P</i><0.05). As age increased, more gonadotropins were consumed (<i>P</i><0.05). * <i>P</i><0.05 versus the short protocol group.</p

Comparison of No. of high-quality embryos.

<p>The number of high-quality embryos in the long protocol group was significantly greater than that in the short protocol group for all age ranges (<i>P</i><0.05). This value significantly decreased with increasing age (<i>P</i><0.05). * <i>P</i><0.05 versus the short protocol group.</p

Additional file 1 of Impact of dehydroepiandrosterone sulfate and free androgen index on pregnancy and neonatal outcomes in PCOS patients

Supplementary Material

Comparison of clinical pregnancy rate.

<p>In the long protocol group, the clinical pregnancy rates of the four age ranges were 52.76%, 44.33%, 36.15% and 13.33%, respectively, which were significantly higher than those in the short protocol group (33.33%, 24.58%, 22.49% and 8.72%, respectively; <i>P</i><0.05). As aged increased, the clinical pregnancy rate in the long protocol group significantly decreased (<i>P</i><0.05). * <i>P</i><0.05 versus the short protocol group.</p

Short versus Long Gonadotropin-Releasing Hormone Analogue Suppression Protocols in IVF/ICSI Cycles in Patients of Various Age Ranges

<div><p>Objective</p><p>To compare the two GnRH-a protocols (long GnRH-a protocol and short GnRH-a protocol) for ovarian stimulation in IVF/ICSI cycles in patients of various age ranges.</p><p>Methods</p><p>A total of 5662 IVF-ET/ICSI cycles from 2010 to 2013 were retrospectively identified. The cycles were divided into two groups: a long protocol group and short protocol group. In each group, the patients were divided into four age ranges: <31 years, 31 to 35 years, 36 to 40 years, and >40 years. The duration of stimulation, total dose of Gn, implantation rate and pregnancy rate were compared.</p><p>Results</p><p>The total dose of Gn was significantly higher, and the duration of stimulation was significantly longer, in the long protocol group than in the short protocol group for all age ranges (<i>P</i><0.05). If the patients were of the same age range, the number of oocytes retrieved, MII oocytes, and high-quality embryos in the long protocol group were all significantly greater than those in the short protocol group (<i>P</i><0.05). In the long protocol group, the clinical pregnancy rates of the four age ranges were 52.76%, 44.33%, 36.15% and 13.33%, respectively, which were significantly higher than those in the short protocol group (33.33%, 24.58%, 22.49% and 8.72%, respectively; <i>P</i><0.05). The same trend was also found in the implantation rates of the four age ranges. As the age increased, the clinical pregnancy and implantation rates, as well as the number of oocytes retrieved, MII oocytes, and high-quality embryos, of the long protocol group significantly decreased (<i>P</i><0.05).</p><p>Conclusions</p><p>Our study demonstrated that regardless of patient age, the long protocol was superior to the short protocol in terms of the number of retrieved oocytes, as well as the implantation and pregnancy rates.</p></div

Comparison of stimulation duration.

<p>The duration of stimulation was significantly longer in the long protocol group than that in the short protocol group for all age ranges (<i>P</i><0.05). As age increased, the durations remained the same. * <i>P</i><0.05 versus the short protocol group.</p

Comparison of No. of oocytes retrieved.

<p>Within each age range, the number of retrieved oocytes in the long protocol group was significantly greater than those in the short protocol group (<i>P</i><0.05). They decreased with increasing age, and the differences were significant according to one-way ANOVA analysis (<i>P</i><0.05). * <i>P</i><0.05 versus the short protocol group.</p

Comparison of miscarriage rate.

<p>For all age ranges, there were no significant differences in miscarriage rates between the two groups. In the short protocol group in patients older than 40 years, the miscarriage rate was 52.94%, which was significantly higher than that of the other three age ranges (6.90%, 22.73% and 16.07%, respectively; <i>P</i><0.05).</p

Table1_A comprehensive preimplantation genetic testing approach for SEA-type α-thalassemia by fluorescent gap-polymerase chain reaction combined with haplotype analysis.pdf

Introduction: This study aimed to evaluate the feasibility and necessity of using fluorescence Gap-polymerase chain reaction combined with haplotype analysis in preimplantation genetic testing for SEA-type α-thalassemia.Methods: A total of 26 preimplantation genetic testing biopsy cycles were performed in 25 families from June 2021 to February 2022. All couples were carriers of SEA-type α-thalassemia. Fluorescent Gap-polymerase chain reaction was used for detecting fragment deletion. Subsequently, according to the results of polymerase chain reaction, reference embryos were identified to establish haplotype using single nucleotide polymorphism array, and aneuploidy was screened simultaneously. In cases wherein the polymerase chain reaction results were inconsistent with the haplotype results, the reasons were investigated, either by retest of the biopsied samples or rebiopsy of the embryo.Results: Among the 172 embryos, 162 had consistent results when tested using both methods, resulting in a consistency rate of 94.2%. Conversely, 10 embryos had inconsistent results, mainly due to chromosome 16 aneuploidy (n = 7), allele dropout in Gap-polymerase chain reaction (n = 2), or incorrect haplotype due to poor sample amplification quality (n = 1). The clinical pregnancy rate of each frozen-thawed embryo transfer was 57.7% (15/26). Six families underwent prenatal diagnosis, which confirmed the results of preimplantation genetic testing.Conclusion: Fluorescent Gap-polymerase chain reaction combined with haplotype analysis is feasible and necessary for SEA-type α-thalassemia preimplantation genetic testing.</p

Comparison of implantation rate.

<p>In the long protocol group, the implantation rates of the four age ranges were significantly higher than those in the short protocol group (P<0.05). As aged increased, the implantation rates of the long protocol group significantly decreased (P<0.05). * <i>P</i><0.05 versus the short protocol group.</p