223 research outputs found

    Economic Competition and the Energy Sector: The Electricity and Natural Gas Markets

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    Despite the recent energy reforms in Mexico, the conditions under which some economic sectors operate in Mexico reflect a reality that is still far from a competitive marketplace, and the benefits that such markets generate. The authors of this paper present three factors that, if not recognized in a timely manner, may limit the effective application of the most recent reform in the natural gas and electricity industries. This paper is part of a Baker Institute Mexico Center research project examining the rule of law in Mexico (http://www.bakerinstitute.org/the-rule-of-law-and-mexicos-energy-reform/) and the challenges it poses to implementing the country's energy reform

    Genomic contextualisation of ancient DNA molecular data from an Argentinian fifth pandemic Vibrio cholerae infection.

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    Specific lineages of serogroup O1 Vibrio cholerae are notorious for causing cholera pandemics, of which there have been seven since the 1800s. Much is known about the sixth pandemic (1899‚Äď1923) and the ongoing seventh pandemic (1961‚Äďpresent), but we know very little about the bacteriology of pandemics 1 to 5. Moreover, although we are learning about the contribution of non-O1 non-pandemic V. cholerae to cholera dynamics during the current pandemic, we know almost nothing about their role in the past. A recent ancient DNA study has presented what may be the first molecular evidence of a V. cholerae infection from the fifth cholera pandemic period (1886‚Äď1887 AD) in Argentina. Here, we place the molecular evidence from that study into the genomic context of non-pandemic V. cholerae from Latin America and elsewhere, and show that a gene fragment amplified from ancient DNA is most similar to that of V. cholerae from the Americas, and from Argentina. Our results corroborate and reinforce the findings of the original study, and collectively suggest that even in the 1880s, non-pandemic V. cholerae local to the Americas may have caused sporadic infections in Argentina, just as we know this to have happened during the seventh pandemic in Latin America

    Draft genome sequence of Lactobacillus helveticus ATCC 12046

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    Lactobacillus helveticus is a lactic acid bacterium used traditionally in the dairy industry, especially in the manufacture of cheeses. We present here the 2,141,841-bp draft genome sequence of L. helveticus strain ATCC 12046, a potential starter strain for improving cheese production.Fil: Ruzal, Sandra M√≥nica. Consejo Nacional de Investigaciones Cient√≠ficas y T√©cnicas. Oficina de Coordinaci√≥n Administrativa Ciudad Universitaria. Instituto de Qu√≠mica Biol√≥gica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Qu√≠mica Biol√≥gica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Palomino, Maria Mercedes. Consejo Nacional de Investigaciones Cient√≠ficas y T√©cnicas. Oficina de Coordinaci√≥n Administrativa Ciudad Universitaria. Instituto de Qu√≠mica Biol√≥gica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Qu√≠mica Biol√≥gica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fina Martin, Joaquina. Consejo Nacional de Investigaciones Cient√≠ficas y T√©cnicas. Oficina de Coordinaci√≥n Administrativa Ciudad Universitaria. Instituto de Qu√≠mica Biol√≥gica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Qu√≠mica Biol√≥gica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fern√°ndez Do Porto, Dar√≠o Augusto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Formaci√≥n e Investigaci√≥n en Ense√Īanza de las Ciencias; Argentin

    Alternative end-joining originates stable chromosome aberrations induced by etoposide during targeted inhibition of DNA-PKcs in ATM-deficient tumor cells

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    ATM and DNA-PKcs coordinate the DNA damage response at multiple levels following the exposure to chemotherapy. The Topoisomerase II poison etoposide (ETO) is an effective chemotherapeutic agent that induces DNA double-strand breaks (DSB), but it is responsible from the chromosomal rearrangements frequently found in therapy-related secondary tumors. Targeted inhibition of DNA-PKcs in ATM-defective tumors combined with radio- or chemotherapy has been proposed as relevant therapies. Here, we explored the DNA repair mechanisms and the genetic consequences of targeting the non-oncogenic addiction to DNA-PKcs of ATM-defective tumor cells after exposure to ETO. We demonstrated that chemical inhibition of DNA-PKcs followed by treatment with ETO resulted in the accumulation of chromatid breaks and decreased mitotic index in both A-T cells and ATM-knocked-down (ATMkd) tumor cells. The HR repair process in DNA-PKcs-inhibited ATMkd cells amplified the RAD51 foci number, with no correlated increase in sister chromatid exchanges. The analysis of post-mitotic DNA lesions presented an augmented number of persistent unresolved DSB, without alterations in the cell cycle progression. Long-term examination of chromosome aberrations revealed a strikingly high number of chromatid and chromosome exchanges. By using genetic and pharmacological abrogation of PARP-1, we demonstrated that alternative end-joining (alt-EJ) repair pathway is responsible for those chromosome abnormalities generated by limiting c-NHEJ activities during directed inhibition of DNA-PKcs in ATM-deficient cells. Targeting the non-oncogenic addiction to DNA-PKcs of ATM-defective tumors stimulates the DSB repair by alt-EJ, which is liable for the origin of cells carrying stable chromosome aberrations that may eventually restrict the therapeutic strategy.Fil: de Campos Nebel, Ildefonso Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Palmitelli, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Perez Maturo, Josefina. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; ArgentinaFil: Gonzalez Cid, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentin

    Predicting 6-Month Mortality in Incident Elderly Dialysis Patients: A Simple Prognostic Score

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    Aim: Mortality in end-stage renal disease (ESRD) remains high, particularly among elderly, who represents the most rapidly growing segment of the ESRD population in wealthier countries. We developed and validated a risk score in elderly patients to predict 6-month mortality after dialysis initiation. Methods: We used data from a cohort of 421 patients, aged 65 years and over who started dialysis between 2009 and 2016, in our Nephrology department. The predictive score was developed using a multivariable logistic regression analysis. A bootstrapping technique was used for internal validation. Results: The overall mortality within 6 months was 14.0%. Five independent predictors were identified, and a points system was constructed: age 75 years or older (2 points), coronary artery disease (2), cerebrovascular disease with hemiplegia (2), time of nephrology care before dialysis (<3.0 months [2]; ‚Č•3 to <12 months [1]), and serum albumin levels (3.0-3.49 g/dL [1]; <3.0 g/dL [2]). A score of 6 identified patients with a 70% risk of 6-month mortality. Model performance was good in both discrimination (area under the curve of 0.793; [95% CI 0.73-0.86]) and validation (concordance statistics of 0.791 [95% CI 0.73-0.85]). Conclusions: We developed a simple prediction score based on readily available clinical and laboratory data that can be a practical and useful tool to assess short-term prognosis in elderly patients starting dialysis. It may help to inform patients and their families about ESRD treatment options and provide a more patient-centered overall approach to care.info:eu-repo/semantics/publishedVersio

    AVALIANDO O PAPEL DOS CONHECIMENTOS PR√ČVIOS PARA ELABORA√á√ÉO DE HIP√ďTESES EM QUEST√ēES ABERTAS NO ENSINO DE F√ćSICA

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    Nesta comunica√ß√£o apresentamos uma pesquisa desenvolvida com uma turma do ensino m√©dio integrado de uma escola da rede federal de ensino da cidade de Manaus. O obetivo da pesquisa foi de avaliar qual o papal exercido pelos conhecimentos pr√©vios para elabora√ß√£o de hip√≥teses em quest√Ķs abertas no ensino de f√≠sica. Constatamos que o conhecimento pr√©vio √© uma condi√ß√£o necess√°ria mas n√£o suficiente, pois possibilitou ao alunado formularem as hip√≥teses embora, em sua maioria, elas n√£o estivessem adequadas ao que √© posto pela f√≠sica. Isto indica que o senso comum foi o conhecimento mais utilizado para formul√°-las ao inv√©s do conhecimento cient√≠fico, como esperado

    Estudio de comportamiento y necesidades de informaci√≥n en mujeres emprendedoras. Retos actuales para las Bibliotecas P√ļblicas cubanas.

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    El proceso de transformaciones socioecon√≥micas que se viene implementando en Cuba en los √ļltimos a√Īos ha permitido la ampliaci√≥n de actores econ√≥micos, resultando de gran relevancia el aumento acelerado del sector no estatal, concebido este como un actor clave para el desarrollo del pa√≠¬≠s. Estos cambios en las formas de empleo han venido acompa√Īados de un conjunto de nuevas reglamentaciones, normativas que ha incidido en la emergencia de nuevas necesidades de informaci√≥n y aprendizaje en gran parte de la sociedad cubana relacionada al desarrollo de emprendimientos, teniendo en cuenta adem√°s el vertiginoso avance de las Tecnolog√≠¬≠as de la Informaci√≥n y las Comunicaciones (TIC), necesarias cada vez m√°s para la gesti√≥n de los emprendimientos y favorecer el acceso a la informaci√≥n. El escenario actual demanda una contextualizaci√≥n de los servicios que ofrecen las bibliotecas p√ļblicas a fin de satisfacer las necesidades de informaci√≥n y de aprendizaje que emergen en las comunidades a partir de las oportunidades para el emprendimiento. Con este prop√≥sito se indag√≥ en las necesidades de informaci√≥n y comportamiento informacional de mujeres emprendedoras residentes en comunidades con riesgo a la vulnerabilidad social, donde el proceso para el desarrollo de los emprendimientos supone dis√≠¬≠miles desventajas, en particular para las mujeres por su posici√≥n hist√≥rica en la divisi√≥n sexual del trabajo. A partir de los resultados recabados a trav√©s de encuestas y entrevistas en profundidad, se presenta un conjunto de propuestas para los servicios de las bibliotecas p√ļblicas en articulaci√≥n con algunas instituciones de la comunidad, entre ellos los Joven Club de Computaci√≥n y Electr√≥nica, en aras de aprovechar las capacidades tecnol√≥gicas de estos √ļltimos que a su vez implican nuevas formas de aprendizaje, al integrar el uso de las TIC para el desenvolvimiento de los emprendimientos, desde una perspectiva de equidad de g√©nero e inclusi√≥n social.
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