Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Decontamination and Ecological Restoration Performance of a Bioretention Cell-Microbial Fuel Cell under Multiple-Antibiotics Stress

Antibiotics are refractory pollutants that have been widely found in various environmental media such as soil and surface water. Existing sewage treatments perform poorly at preventing antibiotics in urban sewage from polluting natural environments. In this study, we designed a bioelectrically enhanced bioretention cell system (bioretention cell-microbial fuel cell, BRC-MFC) that utilizes the unique structure of the BRC system to improve the removal of sewage antibiotics. This new system can efficiently remove antibiotics by using a synergy of plant absorption, filler adsorption, filler filtration and microbial degradation. To study the influences of multiple-antibiotics stress on the decontamination performance of BRC-MFC, ofloxacin (OFLX) and tetracycline (TC) were selected as target antibiotics, and five BRC-MFCs were built to treat sewage containing antibiotics of different concentrations. The concentrations of pollutant in the influent and effluent were measured and the pollutant removal performance of BRC-MFC was studied. The diversity of rhizosphere microorganisms and the abundance of denitrifying functional genes were analyzed. Experimental results showed that over 90% of OFLX and TC in each BRC-MFC were removed, with the removal rates positively correlating with the concentration of antibiotics. In addition, the removal rates of chemical oxygen demand (COD) in BRC-MFC were both over 90%, while the removal rate of total nitrogen (TN) was around 70%. Meanwhile, antibiotics could significantly improve the removal of ammonia nitrogen (NH4+-N, p &lt; 0.01). The microbial richness decreased, and we found that combined antibiotic stress on microorganisms was stronger than single antibiotic stress. The abundance of denitrifying functional genes was reduced by antibiotic stress. The results of this study provide reference values for other projects focusing on removing various antibiotics from domestic sewage using BRC-MFC.</jats:p

Decontamination and Ecological Restoration Performance of a Bioretention Cell-Microbial Fuel Cell under Multiple-Antibiotics Stress

Antibiotics are refractory pollutants that have been widely found in various environmental media such as soil and surface water. Existing sewage treatments perform poorly at preventing antibiotics in urban sewage from polluting natural environments. In this study, we designed a bioelectrically enhanced bioretention cell system (bioretention cell-microbial fuel cell, BRC-MFC) that utilizes the unique structure of the BRC system to improve the removal of sewage antibiotics. This new system can efficiently remove antibiotics by using a synergy of plant absorption, filler adsorption, filler filtration and microbial degradation. To study the influences of multiple-antibiotics stress on the decontamination performance of BRC-MFC, ofloxacin (OFLX) and tetracycline (TC) were selected as target antibiotics, and five BRC-MFCs were built to treat sewage containing antibiotics of different concentrations. The concentrations of pollutant in the influent and effluent were measured and the pollutant removal performance of BRC-MFC was studied. The diversity of rhizosphere microorganisms and the abundance of denitrifying functional genes were analyzed. Experimental results showed that over 90% of OFLX and TC in each BRC-MFC were removed, with the removal rates positively correlating with the concentration of antibiotics. In addition, the removal rates of chemical oxygen demand (COD) in BRC-MFC were both over 90%, while the removal rate of total nitrogen (TN) was around 70%. Meanwhile, antibiotics could significantly improve the removal of ammonia nitrogen (NH4+-N, p < 0.01). The microbial richness decreased, and we found that combined antibiotic stress on microorganisms was stronger than single antibiotic stress. The abundance of denitrifying functional genes was reduced by antibiotic stress. The results of this study provide reference values for other projects focusing on removing various antibiotics from domestic sewage using BRC-MFC

Decontamination and Ecological Restoration Performance of a Bioretention Cell-Microbial Fuel Cell under Multiple-Antibiotics Stress

Antibiotics are refractory pollutants that have been widely found in various environmental media such as soil and surface water. Existing sewage treatments perform poorly at preventing antibiotics in urban sewage from polluting natural environments. In this study, we designed a bioelectrically enhanced bioretention cell system (bioretention cell-microbial fuel cell, BRC-MFC) that utilizes the unique structure of the BRC system to improve the removal of sewage antibiotics. This new system can efficiently remove antibiotics by using a synergy of plant absorption, filler adsorption, filler filtration and microbial degradation. To study the influences of multiple-antibiotics stress on the decontamination performance of BRC-MFC, ofloxacin (OFLX) and tetracycline (TC) were selected as target antibiotics, and five BRC-MFCs were built to treat sewage containing antibiotics of different concentrations. The concentrations of pollutant in the influent and effluent were measured and the pollutant removal performance of BRC-MFC was studied. The diversity of rhizosphere microorganisms and the abundance of denitrifying functional genes were analyzed. Experimental results showed that over 90% of OFLX and TC in each BRC-MFC were removed, with the removal rates positively correlating with the concentration of antibiotics. In addition, the removal rates of chemical oxygen demand (COD) in BRC-MFC were both over 90%, while the removal rate of total nitrogen (TN) was around 70%. Meanwhile, antibiotics could significantly improve the removal of ammonia nitrogen (NH4+-N, p &lt; 0.01). The microbial richness decreased, and we found that combined antibiotic stress on microorganisms was stronger than single antibiotic stress. The abundance of denitrifying functional genes was reduced by antibiotic stress. The results of this study provide reference values for other projects focusing on removing various antibiotics from domestic sewage using BRC-MFC

Characteristics and Driving Factors of Nitrogen-Use Efficiency in Chinese Greenhouse Tomato Cultivation

Excessive nitrogen fertilizer application in greenhouses could cause a significant variation in the nitrogen-use efficiency at the regional scale. This study aims to quantify agronomic nitrogen-use efficiency (AEN) and identify its driving factors across Chinese greenhouse tomato cultivation. Three hundred and forty-eight AEN values were obtained from 64 papers, including mineral nitrogen (MN) and mineral combined with organic nitrogen (MON) treatments. The average AEN values for the MN and MON treatments were 56.6 ± 7.0 kg kg−1 and 34.6 ± 3.5 kg kg−1, respectively. The AEN of the MN treatment was higher than that of the MON treatment for cultivation using soil with an organic matter content of less than 10 g kg−1 and the drip fertigation method. The AENs of the MN and MON treatments were divided into two segments according to the nitrogen application rate. The inflection points of the nitrogen application rate were 290 and 1100 kg N ha−1 for the MN and MON treatments, respectively. When the ratio of organic nitrogen to total nitrogen was less than 0.4, it was beneficial for improving the AEN. The soil organic matter content and the nitrogen application rate were the most critical factors determining the AEN. These results suggest that rationally reducing the nitrogen input and partially substituting mineral nitrogen with organic nitrogen can help improve the nitrogen-use efficiency.</jats:p

Large crop production losses induced by global ozone stress based on interval evaluation

Abstract Global crop yield loss due to ground-level ozone (O3) concentrations is a major challenge to food security, but a dose-response association is not easy to quantify. Here, we propose using a new metric, O3 sensitivity of crop yield (Yo), to estimate yield loss under different O3 time intervals using four observational databases. The Yo metric shows a non-linear parabola with elevated atmospheric O3 for wheat, maize, rice, soybean, and assorted vegetables. Spatial heterogeneity of yield loss varies as a function of crop type and O3 intervals. Estimates of yield loss from ozone suggest recent losses (2017-2019) may reach as high as 537 million tonnes, with a significant proportion coming with lower (30-40 ppb) exposure (325 million tonnes). Our results suggest that previous research, which only included higher (&gt;40 ppb ozone), may have had grossly underestimated the negative effect of atmospheric O3 on crop production. Suppose these results are endemic to global crop production. In that case, additional research will be necessary to reassess ozone sensitivity and dose-responses, both spatially and temporally, to determine future air pollution impacts.</jats:p

Characteristics and Driving Factors of Nitrogen-Use Efficiency in Chinese Greenhouse Tomato Cultivation

Excessive nitrogen fertilizer application in greenhouses could cause a significant variation in the nitrogen-use efficiency at the regional scale. This study aims to quantify agronomic nitrogen-use efficiency (AEN) and identify its driving factors across Chinese greenhouse tomato cultivation. Three hundred and forty-eight AEN values were obtained from 64 papers, including mineral nitrogen (MN) and mineral combined with organic nitrogen (MON) treatments. The average AEN values for the MN and MON treatments were 56.6 &plusmn; 7.0 kg kg&minus;1 and 34.6 &plusmn; 3.5 kg kg&minus;1, respectively. The AEN of the MN treatment was higher than that of the MON treatment for cultivation using soil with an organic matter content of less than 10 g kg&minus;1 and the drip fertigation method. The AENs of the MN and MON treatments were divided into two segments according to the nitrogen application rate. The inflection points of the nitrogen application rate were 290 and 1100 kg N ha&minus;1 for the MN and MON treatments, respectively. When the ratio of organic nitrogen to total nitrogen was less than 0.4, it was beneficial for improving the AEN. The soil organic matter content and the nitrogen application rate were the most critical factors determining the AEN. These results suggest that rationally reducing the nitrogen input and partially substituting mineral nitrogen with organic nitrogen can help improve the nitrogen-use efficiency