Subdivision of arthropod cap-n-collar expression domains is restricted to Mandibulata

Background: The monophyly of Mandibulata - the division of arthropods uniting pancrustaceans and myriapods - is consistent with several morphological characters, such as the presence of sensory appendages called antennae and the eponymous biting appendage, the mandible. Functional studies have demonstrated that the patterning of the mandible requires the activity of the Hox gene Deformed and the transcription factor cap-n-collar (cnc) in at least two holometabolous insects: the fruit fly Drosophila melanogaster and the beetle Tribolium castaneum. Expression patterns of cnc from two non-holometabolous insects and a millipede have suggested conservation of the labral and mandibular domains within Mandibulata. However, the activity of cnc is unknown in crustaceans and chelicerates, precluding understanding of a complete scenario for the evolution of patterning of this appendage within arthropods. To redress these lacunae, here we investigate the gene expression of the ortholog of cnc in Parhyale hawaiensis, a malacostracan crustacean, and two chelicerates: the harvestman Phalangium opilio, and the scorpion Centruroides sculpturatus. Results: In the crustacean P. hawaiensis, the segmental expression of Ph-cnc is the same as that reported previously in hexapods and myriapods, with two distinct head domains in the labrum and the mandibular segment. In contrast, Po-cnc and Cs-cnc expression is not enriched in the labrum of either chelicerate, but instead is expressed at comparable levels in all appendages. In further contrast to mandibulate orthologs, the expression domain of Po-cnc posterior to the labrum is not confined within the expression domain of Po-Dfd. Conclusions: Expression data from two chelicerate outgroup taxa suggest that the signature two-domain head expression pattern of cnc evolved at the base of Mandibulata. The observation of the archetypal labral and mandibular segment domains in a crustacean exemplar supports the synapomorphic nature of mandibulate cnc expression. The broader expression of Po-cnc with respect to Po-Dfd in chelicerates further suggests that the regulation of cnc by Dfd was also acquired at the base of Mandibulata. To test this hypothesis, future studies examining panarthropod cnc evolution should investigate expression of the cnc ortholog in arthropod outgroups, such as Onychophora and Tardigrada

Transcriptional and Proteomic Analysis of a Ferric Uptake Regulator (Fur) Mutant of Shewanella oneidensis: Possible Involvement of Fur in Energy Metabolism, Transcriptional Regulation, and Oxidative Stress

The iron-directed, coordinate regulation of genes depends on the fur (ferric uptake regulator) gene product, which acts as an iron-responsive, transcriptional repressor protein. To investigate the biological function of a fur homolog in the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1, a fur knockout strain (FUR1) was generated by suicide plasmid integration into this gene and characterized using phenotype assays, DNA microarrays containing 691 arrayed genes, and two-dimensional polyacrylamide gel electrophoresis. Physiological studies indicated that FUR1 was similar to the wild-type strain when they were compared for anaerobic growth and reduction of various electron acceptors. Transcription profiling, however, revealed that genes with predicted functions in electron transport, energy metabolism, transcriptional regulation, and oxidative stress protection were either repressed (ccoNQ, etrA, cytochrome b and c maturation-encoding genes, qor, yiaY, sodB, rpoH, phoB, and chvI) or induced (yggW, pdhC, prpC, aceE, fdhD, and ppc) in the fur mutant. Disruption of fur also resulted in derepression of genes (hxuC, alcC, fhuA, hemR, irgA, and ompW) putatively involved in iron uptake. This agreed with the finding that the fur mutant produced threefold-higher levels of siderophore than the wild-type strain under conditions of sufficient iron. Analysis of a subset of the FUR1 proteome (i.e., primarily soluble cytoplasmic and periplasmic proteins) indicated that 11 major protein species reproducibly showed significant (P < 0.05) differences in abundance relative to the wild type. Protein identification using mass spectrometry indicated that the expression of two of these proteins (SodB and AlcC) correlated with the microarray data. These results suggest a possible regulatory role of S. oneidensis MR-1 Fur in energy metabolism that extends the traditional model of Fur as a negative regulator of iron acquisition systems

An enhanced synchronized multi-channel MAC scheme to improve throughput in VANET

The development of autonomous driving and intelligent transportation demands, fast, reliable and efficient data transmission for various applications in vehicular ad hoc network (VANET). This poses great challenges to the design of a media access control (MAC) protocol that can adapt to the frequent topological shifts. IEEE 1609.4 defines the multi-channel MAC layer implementation in VANET. The multi-channel operation works on a fixed synchronization interval that alternates between a control channel and service channels. The fixed interval leads to poor utilization of limited spectrum resources. In this paper, a multi-channel reliable MAC protocol (MCRMAC), that uses both the control channel and service channel irrespective of the interval to ensure proper and efficient throughput utilization in VANET is proposed. The simulations reveal the performance of the proposed scheme and it outperforms IEEE 1609.4 in terms of throughput

Dissection of Organs from the Adult Zebrafish

Over the last 20 years, the zebrafish has become a powerful model organism for understanding vertebrate development and disease. Although experimental analysis of the embryo and larva is extensive and the morphology has been well documented, descriptions of adult zebrafish anatomy and studies of development of the adult structures and organs, together with techniques for working with adults are lacking. The organs of the larva undergo significant changes in their overall structure, morphology, and anatomical location during the larval to adult transition. Externally, the transparent larva develops its characteristic adult striped pigment pattern and paired pelvic fins, while internally, the organs undergo massive growth and remodeling. In addition, the bipotential gonad primordium develops into either testis or ovary. This protocol identifies many of the organs of the adult and demonstrates methods for dissection of the brain, gonads, gastrointestinal system, heart, and kidney of the adult zebrafish. The dissected organs can be used for in situ hybridization, immunohistochemistry, histology, RNA extraction, protein analysis, and other molecular techniques. This protocol will assist in the broadening of studies in the zebrafish to include the remodeling of larval organs, the morphogenesis of organs specific to the adult and other investigations of the adult organ systems

Ricinus communis and Calotropis procera As Putative Plant Species for the Phytostabilization of Tannery Contaminated Soil: A Dynamic Approach

The present study involves the assessment of four metals (Cr, Pb, Cu, and Mn) and their mobility (primary and dynamic translocation and bioconcentration factors) in Ricinus communis and Calotropis procera growing in tannery contaminated soil (TCS) and control soil (CS). The area is moderately to strongly contaminated withCr. Except for Cr, all the analyzed metals were found within the critical range in TCS and in both plants. The assessment of both primary and dynamic translocation and bioconcentration factors showed TF &lt; 1 and BCF &gt; 1 for both plants, which demonstrates the major transfer and accumulation of Cr from soil to root. As these plants are not grazed upon by grazing animals, the ecological metal transfer risks from these plants are quite low. Moreover, the high commercial importance of these plants (biofuel production and medicinal value) further enhances their utilization for the phytostabilization of moderately Cr-contaminated sites. Keywords: chromium, Ricinus communis, Calotropis procera, dynamic factors, tannery industr

Why Are Outcomes Different for Registry Patients Enrolled Prospectively and Retrospectively? Insights from the Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF).

Background: Retrospective and prospective observational studies are designed to reflect real-world evidence on clinical practice, but can yield conflicting results. The GARFIELD-AF Registry includes both methods of enrolment and allows analysis of differences in patient characteristics and outcomes that may result. Methods and Results: Patients with atrial fibrillation (AF) and ≥1 risk factor for stroke at diagnosis of AF were recruited either retrospectively (n = 5069) or prospectively (n = 5501) from 19 countries and then followed prospectively. The retrospectively enrolled cohort comprised patients with established AF (for a least 6, and up to 24 months before enrolment), who were identified retrospectively (and baseline and partial follow-up data were collected from the emedical records) and then followed prospectively between 0-18 months (such that the total time of follow-up was 24 months; data collection Dec-2009 and Oct-2010). In the prospectively enrolled cohort, patients with newly diagnosed AF (≤6 weeks after diagnosis) were recruited between Mar-2010 and Oct-2011 and were followed for 24 months after enrolment. Differences between the cohorts were observed in clinical characteristics, including type of AF, stroke prevention strategies, and event rates. More patients in the retrospectively identified cohort received vitamin K antagonists (62.1% vs. 53.2%) and fewer received non-vitamin K oral anticoagulants (1.8% vs . 4.2%). All-cause mortality rates per 100 person-years during the prospective follow-up (starting the first study visit up to 1 year) were significantly lower in the retrospective than prospectively identified cohort (3.04 [95% CI 2.51 to 3.67] vs . 4.05 [95% CI 3.53 to 4.63]; p = 0.016). Conclusions: Interpretations of data from registries that aim to evaluate the characteristics and outcomes of patients with AF must take account of differences in registry design and the impact of recall bias and survivorship bias that is incurred with retrospective enrolment. Clinical Trial Registration: - URL: http://www.clinicaltrials.gov . Unique identifier for GARFIELD-AF (NCT01090362)

Evolving feature model configurations in software product lines

The increasing complexity and cost of software-intensive systems has led developers to seek ways of reusing software components across development projects. One approach to increasing software reusability is to develop a software product-line (SPL), which is a software architecture that can be reconfigured and reused across projects. Rather than developing software from scratch for a new project, a new configuration of the SPL is produced. It is hard, however, to find a configuration of an SPL that meets an arbitrary requirement set and does not violate any configuration constraints in the SPL. Existing research has focused on techniques that produce a configuration of an SPL in a single step. Budgetary constraints or other restrictions, however, may require multi-step configuration processes. For example, an aircraft manufacturer may want to produce a series of configurations of a plane over a span of years without exceeding a yearly budget to add features. This paper provides three contributions to the study of multi-step configuration for SPLs. First, we present a formal model of multi-step SPL configuration and map this model to constraint satisfaction problems (CSPs). Second, we show how solutions to these SPL configuration problems can be automatically derived with a constraint solver by mapping them to CSPs. Moreover, we show how feature model changes can be mapped to our approach in a multi-step scenario by using feature model drift. Third, we present empirical results demonstrating that our CSP-based reasoning technique can scale to SPL models with hundreds of features and multiple configuration steps.Ministerio de Economía y Competitividad TIN2012-32273Junta de Andalucía TIC-590