1,448 research outputs found

    Towards transition management of European water resources

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    Global change fundamentally changes the nature of water-related problems. We will illustrate this by showing how perceptions of the water-problems in the Netherlands have shifted in the past four decades. The nature of water-related problems changed from a technical problem’ to a so-called ‘persistent’ problem, characterized by plurality, uncertainty and complexity. Although integrated water resource management (IWRM) has been advocated to cope with this type of problem, the complexity of the transition process towards such a water management regime is often underestimated. Therefore, transition management is needed in the water sector. Transition management theory is presented and applied to the Dutch case. Transition management strategies are suggested that would reinforce this transition. Comparison between the European Water Framework Directive (WFD) and transition management indicates that the Common Implementation Strategy (CIS) in its current form is not sufficiently stimulating an innovation climate

    Transitiemanagement en duurzame ontwikkeling: co-evolutionaire sturing in het licht van complexiteit

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    This article presents a promising governance framework for 'persistent societal problems', based on the concept of transition management. This framework is based on the common notions from complexity theory and new forms of governance, that are folded into a new management paradigm. This management paradigm starts from complexity and uncertainty as drivers of societal innovation, not as obstacles that have to be fully controlled. Essential feature of transition management is the explicit coupling of content and process. The analysis (based on the presented analytical concepts) determines the transition management process: the management possibilities and instruments that are employed with regard to the presented management framework. The framework entails goals, activities, instruments and competences for the strategic, tactical and operational level. Which activities and actors are being involved depends on the phase of the transition

    Захисні лісові насадження – важливий структурний елемент у формуванні національної екологічної мережі

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    Описано роль і значення захисних лісових насаджень як важливого структурного елементу при формуванні національної екологічної мережі.Описана роль и значение защитных лесных насаждений как важного структурного элемента при формировании национальной экологической сети.Role and meaning of forest protective stands as an important structural element for national ecological network formation is described

    Immunocytochemical localization of the neuron-specific form of the c-src gene product, pp60c-src(+), in rat brain

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    Neurons express high levels of a variant form of the c-src gene product, denoted pp60c-src(+), which contains a 6 amino acid insert in the amino-terminal half of the c-src protein. We have determined the localization of pp60c-src(+) in neurons using an affinity-purified anti-peptide antibody, referred to as affi-SB12, that exclusively recognizes this neuron-specific form of the c-src gene product. Using affi-SB12, we examined the distribution of pp60c-src(+) by immunoperoxidase staining of sections through adult rat brains, pp60c-src(+) was widely distributed in rat brain and appeared to be differentially expressed in subpopulations of neurons. The majority of immunoreactive neurons was found in the mesencephalon, cerebellum, pons, and medulla. Telencephalic structures that contained substantial populations of pp60c-src(+)-immunoreactive neurons included layer V of the cerebral cortex and the ventral pallidum. Within individual neurons, pp60c-src(+) immunoreactivity was localized to the cell soma and dendritic processes, while labeling of axons and nerve terminals (puncta) was not as readily detected. Dense accumulations of immunoreactive axons were rare, being most prominent in portions of the inferior and superior olive, and in the spinal trigeminal nucleus. While the regional distribution of pp60c-src(+) immunoreactivity does not correlate with any specific neuronal cell type or first messenger system, this unique pattern of expression of pp60c-src(+) suggests the existence of a previously uncharacterized functional organization within the brain. Furthermore, the localization of this neuron-specific tyrosine kinase in functionally important areas of the nerve cell, namely, dendritic processes, axons, and nerve terminals, suggests that pp60c-src(+) may regulate pleiotropic functions in specific classes of neurons in the adult central nervous system

    The transition in Dutch water management

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    Over the past decades the Dutch people have been confronted with severe waterrelated problems, which are the result of an unsustainable water system, arising from human interventions in the physical infrastructure of the water system and the water management style. The claims of housing, industry, infrastructure and agriculture have resulted in increasing pressure on the water system. The continuous subsidence of soil and climate change has put pressure on the land. Hence, the nature and magnitude of water-related problems have changed. Longitudinal research of relevant national policy documents reveals that the water management regime has changed its water management style over the past thirty years from a technocratic scientific style towards an integral and participatory style. We have investigated if the historical development in Dutch Water management can be characterized as a transition. Based on longitudinal research through an integrated systems analysis, document research and expert interviews, we have reconstructed the historical narrative by using the transition concepts of multi-level and multi-phase. This research indicates that the shift in Dutch Water management can be characterized as a transition. This transition is currently in the take-off stage and near the acceleration stage. This is a crucial stage as long as the considerable differences between the strategic macro-vision and the practical implementation at the micro-level remains. As long as these levels are not compatible (modulation), the transition will not be completed successfully. Transition management as multi-level governance model should therefore be adopted to facilitate the modulation

    An amphitropic cAMP-binding protein in yeast mitochondria

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    ABSTRACT: We describe the first example of a mitochondrial protein with a covalently attached phos-phatidylinositol moiety acting as a membrane anchor. The protein can be metabolically labeled with both stearic acid and inositol. The stearic acid label is removed by phospholipase D whereupon the protein with the retained inositol label is released from the membrane. This protein is a cAMP receptor of the yeast Saccharomyces cereuisiae and tightly associated with the inner mitochondrial membrane. However, it is converted into a soluble form during incubation of isolated mitochondria with Ca2+ and phospholipid (or lipid derivatives). This transition requires the action of a proteinaceous, N-ethylmaleimide-sensitive component of the intermembrane space and is accompanied by a decrease in the lipophilicity of the cAMP receptor. We propose that the component of the intermembrane space triggers the amphitropic behavior of the mitochondrial lipid-modified CAMP-binding protein through a phospholipase activity. Only in recent years specific fatty acids have been recog-nized to play important roles in the association of proteins with membranes. Both noncovalent and covalent interactions be-tween fatty acids and proteins have been reported. Among the latter are GTP-binding proteins (Molenaar et al., 1988)
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