Additional file 3 of Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella

Additional file 3: Supplementary File 2. The gene alignments and all parameters for the phylogenetic analysis conducted in this study

Additional file 1 of Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella

Additional file 1: Fig. S1. RNA mapping to confirm the single nucleotide deletion in the H. moniliformis MAT1-2-7. Fig. S2. The structure of the Huntiella a-factor pheromone proteins and the sequence of the putative mature repeats. Fig. S3. An alignment of the a1 a-factor pheromone factor genes from all eight Huntiella species considered in this study. Fig. S4. RNA mapping to confirm in frame stop codons in the a1 and a6 a-factor pheromone factor genes from the unisexual H. moniliformis. Fig. S5. An alignment of the a3 a-factor pheromone from all eight Huntiella species considered in this study. Fig. S6. RNA mapping to determine expression of the multiple a-factor pheromone genes from H. abstrusa. Fig. S7. RNA mapping to determine expression of the multiple a-factor pheromone genes from H. omanensis. Fig. S8. RNA mapping to determine expression of the multiple a-factor pheromone genes from H. moniliformis. Fig. S9. The structure of the Huntiella α-factor pheromones from the various Huntiella species along with the sequences of the putative mature repeats

Additional file 2 of Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella

Additional file 2: Supplementary File 1. All of the scripts and parameters used for the genome assemblies produced in this study

Additional file 4 of Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella

Additional file 4: Table S1. Genome sequencing and assembly statistics. Table S2. Comparisons of Huntiella genome statistics. Table S3. Gene present at the second a-factor pheromone locus

Supplementary tables: A high-quality fungal genome assembly resolved from a sample accidentally contaminated by multiple taxa

Table S1 Taxonomic identity of 16 randomly chosen multi-copy Eukarya BUSCOs identified in CMW55930 Assembly 1 Table S2 Identification of the Penicillium BenA (beta-tubulin) gene in CMW55930 Assembly 1 Table S3 BUSCOs identified in MAG1-3 when including and excluding contigs classified by EukRep as prokaryotic Table S4 Teratosphaeria eucalypti contigs with a putative prokaryotic origin that resemble mitochondrial sequences of T. pseudoeucalypti and T. viscida</i

The diversity of the Leotiomycete a-factor pheromone in the Ploettnerulaceae, Drepanopezizaceae, Mollisiaceae, Erysiphaceae, Hyaloscyphaceae, Helotiaceae and Lachnaceae.

The diversity of the Leotiomycete a-factor pheromone in the Ploettnerulaceae, Drepanopezizaceae, Mollisiaceae, Erysiphaceae, Hyaloscyphaceae, Helotiaceae and Lachnaceae.</p

A practical approach to genome assembly and annotation of Basidiomycota using the exampleof Armillaria: Supplementary Table S1

Supplementary Table 1</p

Alpha pheromone sequences for all Leotiomycetes (FASTA).

Alpha pheromone sequences for all Leotiomycetes (FASTA).</p

Gene alignments and RNAseq mappings of the <i>ste2</i> gene from <i>Hymenoscyphus</i> species.

Gene alignments and RNAseq mappings of the ste2 gene from Hymenoscyphus species.</p

Alpha pheromone sequences for all Leotiomycetes (GBK).

Alpha pheromone sequences for all Leotiomycetes (GBK).</p