Distribution of Di- to Hexa- microsatellite motifs in <i>Anisogramma</i><i>anomala</i> by number of repeats.

<p>Distribution of Di- to Hexa- microsatellite motifs in <i>Anisogramma</i><i>anomala</i> by number of repeats.</p

Frequency distribution of mono-, di-, and tri- microsatellite motifs in <i>Anisogramma</i><i>anomala</i> by motif sequences.

<p>Frequency distribution of mono-, di-, and tri- microsatellite motifs in <i>Anisogramma</i><i>anomala</i> by motif sequences.</p

Experimental screening of microsatellite loci in <i>Anisogramma</i><i>anomala</i>.

<p>For polymorphic loci, the expected size difference between alleles is multiples of the unit size (e.g. 2x, 3x, and 4x for di-, tri- and tetra- nucleotide motifs, respectively), assuming the motifs are perfect and there are no size differences in the flanking regions.</p

Frequency distribution of microsatellite loci in <i>Anisogramma anomala</i> by motif length.

<p>Frequency distribution of microsatellite loci in <i>Anisogramma anomala</i> by motif length.</p

Flow chart describing the bioinformatics pipeline used to construct a genome-wide microsatellite marker database for <i>Anisogramma</i><i>anomala</i>.

<p>See text for detail. </p

Consensus UPGMA tree of 30 <i>Anisogramma</i><i>anomala</i> isolates characterized by 10 microsatellite loci.

<p>Isolates are named by the city and state from where they were collected, and followed by a number if multiple isolates were obtained from the same location. Isolates were grouped into two clades in accordance with their geographic origins. UPGMA trees were generated by PowerMarker version 3.25 [36] using frequency-based genetic distance of shared alleles [39]. Consensus tree was computed from 100 bootstrap replicates using “consense” program in software package PHYLIP version 3.695 (<a href="http://evolution.genetics.washington.edu/phylip.html" target="_blank">http://evolution.genetics.washington.edu/phylip.html</a>). Numbers by the branches are bootstrap support values in 100 replicates. Branches with less than 50% bootstrap support were collapsed. </p

Bacterial lesions are larger on the fungal mutant <i>pth11</i> versus on the wild-type fungus.

<p>Graph shows measurements of lesion diameters caused by the wild-type bacterial strain, C3, on the wild-type fungus, 4091, the <i>Δpth11</i> mutant, and the complemented line, <i>c- Δpth11</i> (A). Bars represent the average of two biological replicates, and the lines represent error bars. Lowercase letters indicate significance at Day 4 (p-value > 0.03) and uppercase letters indicate insignificance at Day 6 (p-value > 0.14). Statistics were performed with Tukey-Kramer HSD test. Images are representative of three biological replicates. <i>M. oryzae</i> mycelia were grown on oatmeal agar for 12 days in 6-well plates (each well is 3.5 cm in diameter) and inoculated with a 40 µl drop C3 (left of center) and a 40 µl drop of DCA (right of center) (B). The image shows lesions 4 days post-inoculation. The DCA mutant bacteria never caused lesions, while the C3 wild-type strain caused large lesions on the <i>Δpth11</i> mutant.</p

Area proportional Venn diagrams of the differentially expressed genes in <i>M. oryzae</i>.

<p>The fungus was challenged with <i>L. enzymogenes</i> wild-type strain C3 (red circles) and with mutant DCA (green circles) and the transcriptome was profiled at 3 and 9 hpi. The left and right diagrams show number of induced and repressed genes, respectively.</p

Protein motifs for genes repressed by <i>L. enzymogenes</i> wild-type C3, and induced by mutant DCA.

<p>Amino acid sequences of 100 genes were analyzed using the motif finding program MEME, revealing five significant classes. Motifs 1 and 2 were found in MGG_08623.6, MGG_10662.6, and MGG_09601.6. Motif 3 was found in MGG_09857.6, MGG_01231.6, and MGG_00220.6. Motif 4 was found in MGG_14292.6, MGG_01863.6, and MGG_06587.6. Motif 5 was found in MGG_12589.6, MGG_00689.6, MGG_03201.6, and MGG_05025.6.</p

Confocal images of the interaction assay of <i>M. oryzae</i> and <i>L. enzymogenes</i> wild-type strain C3.

<p><i>M. oryzae</i> expressing a green fluorescent protein and <i>L. enzymogenes</i> expressing a dsRed fluorescent protein at 3hpi (A) and 9 hpi (B), and a mock inoculated sample (C). The long, thin structures are hyphae, whereas the tear-drop shaped structures are conidia. The smaller red rod shapes are bacteria. <i>M. oryzae</i> conidium size ranges from 20 to 30 µm. Scale bar: 20µm.</p