Ethylene and the postharvest performance of cut camellia flowering branches

Camellias are potentially appreciated as cut branches due to their deep and bright green foliage with a high number of flower buds. The present research investigated the role of ethylene in postharvest flower and leaf abscission of seven cultivars of Camellia japonica and evaluated the effect of inhibitors of ethylene action on their vase life. Cut flowering branches were shipped from private companies, mimicking a long and short supply chain. Plant materials were treated with commercial Chrysal, gaseous 1-MCP, and 1-MCP included in β-cyclodextrin-based nanosponges and compared to cut branches exposed to exogenous ethylene (1±0.2 μl l-1) for 24 h and control in tap water. Visual checks for symptoms of variation in senescence, flower opening stage, flower abscission, leaf chlorophyll content (SPAD) and gloss, in addition to ethylene production levels were monitored daily. Cut branches placed in tap water lost ornamental value after ca. 5.5 days of vase life and reached complete senescence after about 10 days in all the studied cultivars, regardless of supply chain, with the exception of ‘Il Tramonto’ with 10 and 20 days, respectively. Exogenous ethylene application reduced camellia cut branch longevity in ‘Debbie’ and ‘R.H. Wheeler’, while the other cultivars lasted as long as the control. Anti-ethylene treatments prolonged the vase life only in ‘Korun Koku’ and ‘Il Tramonto’

First Report of Root Rot Caused by <i>Phytophthora cinnamomi</i> on Mountain Laurel (<i>Kalmia latifolia</i>) in Italy

Kalmia latifolia L., common name Mountain Laurel, is an evergreen shrub that is becoming increasingly popular in gardens. It is also grown as a potted plant for its round flowers that range from light pink to white and occur in clusters in late spring. During July 2011, 3-year-old plants of K. latifolia ‘Olympic Fire’ showed extensive chlorosis and root rot on several commercial nurseries close to Maggiore Lake (Verbano-Cusio-Ossola Province) in northwestern Italy. Twigs wilted and died and leaves dropped, although in some cases, wilted leaves persisted on stems. The whole root system was affected with vascular tissues on the lower stem exhibiting brown discoloration, followed by plant death. The disease was severe and widespread, affecting 5% of approximately 3,500 plants. Tissue fragments of 1 mm2 were excised from the margins of the lesions and plated. A Phytophthora-like organism was consistently isolated on an oomycete-selective medium (BNPRA + HMI at 25 μg/ml) (4) after disinfesting root pieces for 1 min in a 1% NaOCl solution. The pathogen was identified based on morphological and physiological features as Phytophthora cinnamomi (2). Oogonia didn't form in single culture. On V8 agar, the microorganism was characterized by coenocytic coralloid hyphae, with spherical lateral and terminal swellings 23 to 46 (average 34) μm in diameter (n = 50), single or in clusters, and produced spherical, terminal chlamydospores 35 to 47 (average 40) μm in diameter (n = 50). No sporangia were produced after growing pure cultures in sterilized soil extracts nor were they produced on V8 agar. The internal transcribed spacer (ITS) region of the rDNA of a single isolate was amplified using the primers ITS1/ITS4 and sequenced. BLAST analysis (1) of the 898-bp segment showed a 99% homology with the sequence of P. cinnamomi (GU799638). The nucleotide sequence has been assigned the GenBank Accession No. JQ951607. Pathogenicity of one isolate obtained from infected plants was confirmed by inoculating 18-month-old plants of K. latifolia ‘Olympic Fire’. The isolate was grown for 50 days in a mixture of 70:30 wheat/hemp kernels and then mixed into a substrate containing sphagnum peat moss/pumice/pine bark/clay (50:20:20:10 v/v) at a rate of 3 g/l. One plant per 2-l pot was transplanted into the substrate and constituted the experimental unit. Five plants were inoculated. Noninoculated plants represented the control treatment and the trial was repeated once. All plants were kept in a greenhouse at 24 to 27°C. Two of five plants inoculated developed symptoms of chlorosis, wilting, and root rot after 70 days and remaining plants after about 80 days. P. cinnamomi was reisolated consistently from inoculated plants but not from controls that remained symptomless. To our knowledge, this is the first report of P. cinnamomi on K. latifolia in Italy and in Europe. The disease has been reported in the United States (3). The economic importance of the disease is uncertain because of the limited number of nurseries that grow this crop in Italy, although its importance could increase as the popularity of K. latifolia increases. References: (1) S. F. Altschul et al. Nucleic Acids Res. 25:3389, 1997 (2) D. C. Erwin and O. K. Ribeiro. Phytophthora Diseases Worldwide. American Phytopathological Society, St Paul, MN, 1996. (3) L. F. Grand. North Carolina Agric. Res. Serv. Techn. Bull. 240, 1985. (4) H. Masago et al. Phytopathology 67:425, 1977. </jats:p

Management of pregnancy in autoimmune rheumatic diseases: maternal disease course, gestational and neonatal outcomes and use of medications in the prospective Italian P-RHEUM.it study

objectives: to investigate pregnancy outcomes in women with autoimmune rheumatic diseases (ARD) in the Italian prospective cohort study P-RHEUM.it. methods: pregnant women with different ARD were enrolled for up to 20 gestational weeks in 29 rheumatology centres for 5 years (2018-2023). maternal and infant information were collected in a web-based database. results: we analysed 866 pregnancies in 851 patients (systemic lupus erythematosus was the most represented disease, 19.6%). maternal disease flares were observed in 135 (15.6%) pregnancies. 53 (6.1%) pregnancies were induced by assisted reproduction techniques, 61 (7%) ended in miscarriage and 11 (1.3%) underwent elective termination. obstetrical complications occurred in 261 (30.1%) pregnancies, including 2.3% pre-eclampsia. two cases of congenital heart block were observed out of 157 pregnancies (1.3%) with anti-Ro/SSA. regarding treatments, 244 (28.2%) pregnancies were treated with glucocorticoids, 388 (44.8%) with hydroxychloroquine, 85 (9.8%) with conventional synthetic disease-modifying anti-rheumatic drugs and 122 (14.1%) with biological disease-modifying anti-rheumatic drugs. Live births were 794 (91.7%), mostly at term (84.9%); four perinatal deaths (0.5%) occurred. among 790 newborns, 31 (3.9%) were small-for-gestational-age and 169 (21.4%) had perinatal complications. exclusive maternal breast feeding was received by 404 (46.7%) neonates. the edinburgh postnatal depression scale was compiled by 414 women (52.4%); 89 (21.5%) scored positive for emotional distress. conclusions: multiple factors including preconception counselling and treat-to-target with pregnancy-compatible medications may have contributed to mitigate disease-related risk factors, yielding limited disease flares, good pregnancy outcomes and frequency of complications which were similar to the Italian general obstetric population. disease-specific issues need to be further addressed to plan preventative measures