Integrative assessment of ecological responses and chemical contamination of urban wastewater outfalls on soft bottom sediments of an estuarine system

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Procedimiento para la criopreservación de embriones de erizo de mar y bioensayo asociado

El procedimiento se basa en el uso de un protocolo de criopreservación para blástulas de 8 horas de erizo de mar para poder almacenar en nitrógeno líquido embriones para su uso fuera de la temporada de reproducción natural de esta especie. Se ha desarrollado además, un bioensayo ecotoxicológico con embriones de erizo de mar criopreservados para la evaluación de la contaminación marina. El procedimiento objeto de esta patente permite el uso durante todo el año de embriones de erizo de mar tanto en bioensayos ecotoxicológicos como con aplicaciones en la industria de la acuicultura

Contaminación

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In vivo oral bioavailability of Pb sequestered in metal rich granules in bivalves

The present study was designed to evaluate in vivo the oral bioavailability of lead (Pb) present in the marine bivalve Dosinia exoleta. This infaunal clam, despite inhabiting in clean areas, presents Pb concentrations that are over the 1.5 mg kg−1 wet weight limit for human consumption set by the European Commission. However, Pb is accumulated in this clam in the form of metal rich granules, and it has been shown to be unavailable for trophic transfer to a marine decapod, so it was hypothesised that it might be unavailable for human consumers as well. Twelve Sprague Dawley rats were fed during 14 days with a diet including control mussels (Mytilus galloprovincialis), D. exoleta, or mussels enriched in Pb to the same levels as those found in D. exoleta. Pb accumulation in different rat tissues (blood, bone, kidneys and liver) was analysed. It was observed that Pb assimilation from D. exoleta was about half of Pb assimilation from M. galloprovincialis, and absolute bioavailabilities were around 2% for M. galloprovincialis and 1% for D. exoleta. These results suggest that it might be possible to increase the limit for human consumption for this bivalve to 3 mg kg−1 wet weight without representing an increase in the risk for consumers

Integrative assessment of coastal pollution: development and evaluation of sediment quality criteria from chemical contamination and ecotoxicological data

Publicado

In vivo oral bioavailability of Pb sequestered in metal rich granules in bivalves

The present study was designed to evaluate in vivo the oral bioavailability of lead (Pb) present in the marine bivalve Dosinia exoleta. This infaunal clam, despite inhabiting in clean areas, presents Pb concentrations that are over the 1.5 mg kg−1 wet weight limit for human consumption set by the European Commission. However, Pb is accumulated in this clam in the form of metal rich granules, and it has been shown to be unavailable for trophic transfer to a marine decapod, so it was hypothesised that it might be unavailable for human consumers as well. Twelve Sprague Dawley rats were fed during 14 days with a diet including control mussels (Mytilus galloprovincialis), D. exoleta, or mussels enriched in Pb to the same levels as those found in D. exoleta. Pb accumulation in different rat tissues (blood, bone, kidneys and liver) was analysed. It was observed that Pb assimilation from D. exoleta was about half of Pb assimilation from M. galloprovincialis, and absolute bioavailabilities were around 2% for M. galloprovincialis and 1% for D. exoleta. These results suggest that it might be possible to increase the limit for human consumption for this bivalve to 3 mg kg−1 wet weight without representing an increase in the risk for consumers