42 research outputs found

    Проблемы аналитического контроля производства платиновых металлов

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    This article is devoted to the analytical control issues of platinum metals production and their solution based on complex approach to the optimization of sampling, sample preparation and analysis stagesРассмотрены проблемы аналитического контроля производства платиновых металлов и их решение, основанное на комплексном подходе к оптимизации этапов пробоотбора, пробоподготовки и химического анализа

    Распространенность бронхиальной астмы среди взрослого населения г. Томска

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    The aim of this study was to estimate prevalence of asthma in adults at industrial city Tomsk. A total of 2000 responders aged 18 to 75 yrs participated in the study. Prevalence of asthma-like symptoms was estimated using the standardized questionnaire ECRHS. Two hundred patients with asthma-like symptoms underwent clinical, functional, and allergological examination. Asthma-like symptoms were revealed in 37.8 % of the studied population. Prevalence of asthma was 9.8 %. These findings significantly exceed official data. Asthma was newly diagnosed in 60 % of symptomatic adults that is evidence of late detection of the disease.Цель данного исследования – изучить распространенность бронхиальной астмы (БА) у взрослого населения в промышленном городе Томске. На 1-м этапе проводился анкетный скрининг 2 000 человек в возрасте от 18 до 75 лет с использованием вопросника ECRHS. На 2-м этапе проводилось клиническое, функциональное и аллергологическое обследование пациентов с астмоподобными симптомами. Астмоподобные симптомы выявлены у 37,8 % человек. Распространенность БА составила 9,8 %, что значительно превышает данные официальной статистики. Впервые выявленная БА была диагностирована в 60 % случаев. Показатели распространенности БА, установленные в ходе эпидемиологического исследования, значительно превосходят данные официальной статистики, что свидетельствует о поздней диагностике БА в общей лечебной сети г. Томска

    Декларация о пациент-ориентированном здравоохранении Томской области

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    The Declaration on Patient-Oriented Healthcare of theTomsk Region was accepted by the medical and patient communities on August 24, 2018. It was the first document of its kind in Russia. It was created based on several elements: modern international experience in the field of protection of patients’ and medical workers’ rights, particular qualities of Russian legislation, principles of humanistic medicine, and broad analysis of specific complaints from patients of regional public health institutions in the Tomsk region. The reason to create the Declaration was the necessity to build a healthcare system in which both medical staff and patients comply with such principles as mutual respect, choice and responsibility, involvement, accessibility, and openness. The document explains the need for constant adherence to these principles for the effectiveness of treatment, the protection of the individual, professional dignity, and equality.Декларация о пациент-ориентированном здравоохранении в Томской области принята медицинским и пациентским сообществами 24 августа 2018 г. Документ подобного рода стал первым в России. Он создан на основе современного мирового опыта в области защиты прав пациентов и медицинских работников, особенностях российского законодательства, принципах гуманистической медицины, широкого анализа конкретных жалоб пациентов областных государственных учреждений здравоохранения Томской области. Создание декларации обусловлено необходимостью выстраивания системы здравоохранения, в которой и медицинские сотрудники, и пациенты соблюдают такие принципы, как взаимоуважение, выбор и ответственность, вовлеченность, доступность, открытость. В документе объясняется необходимость постоянного следования данным принципам для эффективности лечения, защиты личности, профессионального достоинства и равноправия

    THE IMMUNOBIOLOGICAL ACTIVITY OF THE COMPLEX PREPARATION AGAINST HAEMOPHILUS INFLUENZAE INFECTION

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    Aim. To study the immunobiological activity of the complex preparation for Haemophilus influenzae infections prevention. Materials and methods. We used the complex preparation, containing 1 mcg of lipooligosaccharide and 10 mcg of protein-containing fraction, lipooligosaccharide and protein-containing fraction monopreparations, and capsular polysaccharide preparation. For studying cross-protective activity of the complex preparation white outbread mouses (weight 14-16 g) were intramuscularly immunized with a dose 0,5 ml. 10 days later the animals were inoculated with H. influenzae encapsulated and non-typed strains culture inoculum in a dose 5*109 microbial cells/mouse. The immunized animals specific antibodies level was determined by means of indirect enzyme-linked immunosorbent assay (ELISA). For studying complex preparation’s influence on the development of the infectious process immunized mouses were intranasally inoculated with H. influenzae encapsulated and non-encapsulated strains. The mouses dissections were performed in 3, 24 and 72 hours after inoculation. Sterile samples (lung tissue) were homogenized, titrated and plated onto 5% horse blood agar; then after 18-20 growth hours we counted the number of colonies and recalculated its number for one mouse. Results. Our investigations have shown that the complex preparation provided 90-100% animals’ defence from used in this experiment infectious agent strains. Mouses immunization with the preparation induced significant increase in the level of antibodies, revealed by means of indirect ELISA. Beginning with the third day after intranasal inoculation there was a pathogen multiplica112 tion in control group mouses lungs. In the same time immunized mouses had almost indetectable number of bacteria. Almost all animals from control group contained pathogen in lymph nodes and mesentery; though pathogen’s presence in immune mouses viscera was rather occasional. Conclusion. The complex preparation protected animals from all H. influenzae strains, used in our experiment. The dynamic of the infectious process directly depended on the development of immune response on the complex preparation injection

    Models of dependence of the quantity of the <i>Streptococcus pneumoniae biomass</i> and his capsular polysaccharide from the composition of the feeding environment

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    Aim.The development of a semi-synthetic nutrient medium that provides the maximum amount of capsular polysaccharide (CPS). Materials and methods. We used the strain 521 of S. pneumoniae serotype 23F. Cultivation was carried out in test tubes with 10 ml of polysynthetic nutrient medium of a specific composition. The amount of polysaccharide in the samples was determined using rocket immunoelectrophoresis. Building models and comparing the effects of various components was carried out according to the methodology specified in the tutorial. The calculation of the coefficients of the equation and the assessment of the adequacy of the equations themselves was carried out using RStudio version 1.0.153. Results. As a result of a series of experiments, the coefficients of the regression equations were calculated, their significance was evaluated, and models of dependence of biomass production and CPS were constructed depending on the composition of the nutrient medium. To solve the problem, an experiment was carried out according to the Box-Wilson method. The peptone and glucose concentrations were selected as optimized parameters. The step size ΔSi in the increasing gradient direction was calculated based on the coefficients of the regression equation. At the same time, the exact nature of the dependence was determined. The optimal calculated concentrations of peptone and glucose, at which the formation of CPS is maximum, are 32.6 and 12.1 g/l, respectively. In this case, the forecast yield of the polysaccharide is 239 mg/l. Conclusion. Using the method of fractional factorial experiment, models of the dependence of the biomass amount of S. pneumoniae and its capsular polysaccharide on the composition of the nutrient medium were obtained. The optimal concentrations of the components of the medium were found, which make it possible to increase the level of biomass formation by 10% compared to the standard formulas, and the CPS — by 1.5—2 times
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