284 research outputs found

    Exploring Bicycle and Public Transit Use by Low-Income Latino Immigrants: A Mixed-Methods Study in the San Francisco Bay Area

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    Latin American immigrants will continue to make up a large share of transit ridership, bicycling and walking in the United States for the foreseeable future, but there is relatively little research about them. This mixed-methods study compares the travel patterns of low-income immigrants living in the San Francisco Bay Area with that of other groups and investigates the barriers and constraints faced by low-income immigrants when taking transit and bicycling. Much of the previous work on immigrant travel has relied on national surveys and qualitative analysis, which underrepresent disadvantaged population groups and slower modes of travel, or are unable to speak to broader patterns in the population. We conducted interviews with 14 low-income immigrants and a paper-based intercept survey of 2,078 adults. Interviewees revealed five major barriers that made public transit use difficult for them, including safety, transit fare affordability, discrimination, system legibility, and reliability. Although crime was the most prominent issue in interviews, the survey results suggest transit cost is the most pressing concern for low-income immigrants. Low-income immigrants were less likely than those with higher-incomes to have access to a motor vehicle, and were less likely than higher-income immigrants or the U.S.-born of any income to have access to a bicycle or bus pass. Finally, although most barriers to public transit use were the same regardless of nativity or household income, low-income immigrants were much less willing to take public transit when they had the option to drive and less willing to bicycle for any purpose. The prevalence of concerns about transit affordability, crime, and reliability suggest transit agencies should consider income-based fare reductions, coordinated crime prevention with local law enforcement, and improved scheduling

    Giving undocumented immigrants access to driver’s licenses has transportation benefits for everyone.

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    Despite the need for access to a car in most US towns and cities, only sixteen states effectively allow undocumented immigrants to obtain a driver’s license. In new research, Jesus M. Barajas finds that when undocumented immigrants have access to driver’s licenses, there is no change in the number of miles they drive or trips they drive alone, but it does increase the number of carpool trips taken

    Engineered polyketides: Synergy between protein and host level engineering

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    Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities. Polyketides encompass one of the largest classes of chemically diverse natural products. With thousands of known polyketides, modular polyketide synthases (PKSs) share a particularly attractive biosynthetic logic for generating chemical diversity. The engineering of modular PKSs could open access to the deliberate production of both existing and novel compounds. In this review, we discuss PKS engineering efforts applied at both the protein and cellular level for the generation of a diverse range of chemical structures, and we examine future applications of PKSs in the production of medicines, fuels and other industrially relevant chemicals

    Adenoviral gene transfer of interleukin 12 into tumors synergizes with adoptive T cell therapy both at the induction and effector level

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    Tumors infected with a recombinant defective adenovirus expressing interleukin 12 (IL-12) undergo regression, associated with a cytotoxic T lymphocyte (CTL)-mediated antitumor immune response. In the present study we generated anti-CT26 CTLs by short-term coculture of CT26 cells and lymph node cells obtained from mice harboring subcutaneous CT26 tumors injected with an adenoviral vector expressing IL-12 (AdCMVIL-12), control adenovirus (AdCMVlacZ), or saline. Regression of small intrahepatic CT26 tumors in unrelated syngeneic animals was achieved with CTLs derived from mice whose subcutaneous tumors had been injected with AdCMVIL-12 but not with CTLs from the other two control groups. The necessary and sufficient effector cell population for adoptive transfer consisted of CD8+ T cells that showed anti-CT26 specificity partly directed against the AH1 epitope presented by H-2Ld. Interestingly, treatment of a subcutaneous tumor nodule with AdCMVIL-12, combined with intravenous adoptive T cell therapy with short-term CTL cultures, had a marked synergistic effect against large, concomitant live tumors. Expression of IL-12 in the liver in the vicinity of the hepatic tumor nodules, owing to spillover of the vector into the systemic circulation, appeared to be involved in the increased in vivo antitumor activity of injected CTLs. In addition, adoptive T cell therapy improved the outcome of tumor nodules transduced with suboptimal doses of AdCMVIL-12. Our data provide evidence of a strong synergy between gene transfer of IL-12 and adoptive T cell therapy. This synergy operates both at the induction and effector phases of the CTL response, thus providing a rationale for combined therapeutic strategies for human malignancies
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