Study design.

<p>All subjects were scanned twice after 6 hours of fasting to assess short-term reproducibility of MRI metrics (subjects were removed from the MRI system and re-imaged). Subjects were then scanned again in postprandial conditions, 20 min. after a 700 kcal liquid meal.</p

Changes in liver stiffness after a liquid meal (ΔLS*) correlated to changes in portal vein flow (ΔPV Flow*) in healthy volunteers (blue diamonds) and patients (red squares).

<p>There was a significant correlation between ΔLS vs. ΔPV Flow (Spearman rho = 0.48, p = 0.013 for all subjects; rho = 0.51 p = 0.05 for fibrosis patients, and rho = 0.41, p = 0.21 for healthy volunteers). *Δ computed as 100*(postprandial-fasting)/fasting.</p

Postprandial changes in phase contrast metrics (PV flow, PV velocity), DWI metrics (liver D, D*, PF and ADC) and liver stiffness (LS) measured in 30 subjects (expressed in mean ± SD).

<p>PV: portal vein, PV flow (ml/s), PV velocity (cm/s), D (true diffusion coefficient, ×10⁻³ mm²/s), D* (pseudodiffusion coefficient, ×10⁻³ mm²/s), PF (perfusion fraction, %), ADC (apparent diffusion coefficient, ×10⁻³ mm²/s), liver stiffness (liver stiffness, kPa).</p><p>**First fasting measurement.</p><p>***Paired Wilcoxon test.</p><p>****Calculated as 100*(postprandial-fasting)/fasting.</p><p>****LS calculated in 27 subjects.</p

Sequence parameters of the cine phase contrast, DWI and MR elastography (MRE) sequences obtained at 3.0 T in fasting and postprandial states.

<p>Sequence parameters of the cine phase contrast, DWI and MR elastography (MRE) sequences obtained at 3.0 T in fasting and postprandial states.</p

Image processing demonstrated in a 30 year-old healthy volunteer.

<p>Top row demonstrates the region of interest placement in the portal vein (PV) on the phase contrast magnitude and phase images obtained in fasting and postprandial conditions. PV flow and velocity values were 21.15 ml/s/2.7 cm/s (fasting) and 31.1 ml/s/17.8 cm/s (postprandial). Middle row demonstrates the region of interest placement in the right hepatic lobe on the diffusion images (for b = 15) and plots for bi-exponential fitting in fasting (true diffusion coefficient D, pseudodiffusion coefficient D* and perfusion fraction PF of 1.3×10⁻³ mm²/s, 57×10⁻³ mm²/s and 22%) and postprandial conditions (1.1×10⁻³ mm²/s, 171×10⁻³ mm²/s, 19%). Bottom row shows the gradient echo MRE reference image (left) and liver stiffness (LS) maps in fasting and postprandial conditions (LS was 1.9 and 1.9 kPa in fasting and postprandial conditions, respectively).</p

Test-retest reproducibility of pre-bolus and main bolus AIF (arterial input function) shape and corresponding hepatic perfusion parameters measured in 3 patients expressed as mean and range of coefficients of variation (in %).

<p>Test-retest reproducibility of pre-bolus and main bolus AIF (arterial input function) shape and corresponding hepatic perfusion parameters measured in 3 patients expressed as mean and range of coefficients of variation (in %).</p

Sequence parameters of the Look-Locker for T1 mapping, 2D-TurboFLASH for pre-bolus acquisition and 3D-FLASH sequences for main bolus acquisition for liver DCE-MRI.

<p>Sequence parameters of the Look-Locker for T1 mapping, 2D-TurboFLASH for pre-bolus acquisition and 3D-FLASH sequences for main bolus acquisition for liver DCE-MRI.</p

Diagram depicting calculated AIF (arterial input function) parameters (A).

<p>An example is shown in a 67-year-old patient with HCV (same patient as in Fig. 1). Pre-bolus AIF (B) and main bolus AIF (C). Pre-bolus AIF demonstrates higher peak concentration, upslope and AUC60, shorter TTP and smaller FWHM (values are given on the figures).</p

Quantitative AIF (arterial input function) curve parameters obtained for main bolus and pre-bolus acquisitions (mean ± SD).

<p>Quantitative AIF (arterial input function) curve parameters obtained for main bolus and pre-bolus acquisitions (mean ± SD).</p

Quantitative liver perfusion parameters obtained for main bolus and pre-bolus acquisitions (mean ± SD), the coefficients of variation (CV, %) and the Bland-Altman (BA) limits of agreements (%).

<p>Quantitative liver perfusion parameters obtained for main bolus and pre-bolus acquisitions (mean ± SD), the coefficients of variation (CV, %) and the Bland-Altman (BA) limits of agreements (%).</p