Supplementary Figure Legends from The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinoma

Supplementary Figure Legends from The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinom

Supplementary Figure 5 from The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinoma

Nuclear expression of CD9 in breast cancer patients employing an anti-CD9 mAb against a different epitope.</p

Supplementary Figure 1 from The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinoma

Nuclear localization of CD9 in MA-11 and MCF-7 cells.</p

Supplementary Table 1 from The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinoma

Characteristics of patients with ductal carcinoma of the breast. Nuclear CD9 ratios of patients with different stages of ductal breast carcinomas.</p

Supplementary Figure 4 from The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinoma

Abrogation of CD9 expression in MDA-CD9sh cells.</p

Supplementary Figure 2 from The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinoma

Lack of a nuclear CD9 pool in HuMEC.</p

Supplementary Figure 3 from The Nuclear Pool of Tetraspanin CD9 Contributes to Mitotic Processes in Human Breast Carcinoma

MDA, MA-11 and MCF-7 cells do not have a nuclear pool of CD44.</p

Relationship between cell proliferation in culture and number of tumor cells containing four-fold DNA amount.

<p>(A) Inverse relationship (r²>0.95) in U87MG, FEMX-I, MDA-MB-231, and MA11 cell lines, between the number of cells in the G0/G1 peak and that of cells carrying four-fold DNA amount, as quantitated in cultures of each cell line by flow cytometric analysis for content of DNA; both cell populations are expressed as fractions over the total number of cells analysed from each culture. (B) Lack of correlation in cultures of fibroblasts (top panel), <i>vs.</i> negative correlation in cultures of U87MG glioma cells (bottom panel), between number of cells in the G0/G1 peak and number of cells carrying four-fold DNA amount.</p

Matrigel invasion by U87MG, FEMX-I, MDA-MB-231, and MA11 tumor cell lines.

<p>Fluorescence microphotographs of representative areas of the underside of matrigel-coated membranes in transwell assays, showing that U87MG and MDA-MB-231 invade more efficiently through the matrigel, roughly by 4- and 7-fold, respectively, than MA11 and FEMX-I. The cells were fluorescently labeled with 4′,6-diamidino-2-phenylindole, appearing dark in the inverted images. Bars, 100 µm.</p

Relationship between invasiveness and polyploidization in tumor cells.

<p>Graph displaying a proportional ratio (r²>0.95) between the level of polyploidization in the tumor cell lines U87MG, FEMX-I, MDA-MB-231, and MA11, as expressed by the value of the gradient of the regression line, and average density of tumor cell invasion through matrigel.</p