Additional file 4: of Mapping the ‘early salinity response’ triggered proteome adaptation in contrasting rice genotypes using iTRAQ approach

Table S2. Differentially expressed proteins in shoot tissues of Pokkali w.r.t IR64 which are commonly appearing under non stress and stress conditions. (XLSX 13 kb

Additional file 6: of Mapping the ‘early salinity response’ triggered proteome adaptation in contrasting rice genotypes using iTRAQ approach

Table S3. List of RTPCR primers and their sequences (5'to 3') used in this study. (XLSX 10 kb

Additional file 5: of Mapping the ‘early salinity response’ triggered proteome adaptation in contrasting rice genotypes using iTRAQ approach

Figure S3. Depiction of differentially expressed proteins on metabolic pathways using Mapman. Individual elements in the metabolic overview, stress response and redox overview are indicated by solid red rectangular boxes. Rectangular boxes indicate over-represented Mapman functional groups under control and stress conditions in Pokkali with respect to IR64. (TIFF 1525 kb

Putative osmosensor – OsHK3b – a histidine kinase protein from rice shows high structural conservation with its ortholog AtHK1 from <i>Arabidopsis</i>

<div><p>Prokaryotes and eukaryotes respond to various environmental stimuli using the two-component system (TCS). Essentially, it consists of membrane-bound histidine kinase (HK) which senses the stimuli and further transfers the signal to the response regulator, which in turn, regulates expression of various target genes. Recently, sequence-based genome wide analysis has been carried out in <i>Arabidopsis</i> and rice to identify all the putative members of TCS family. One of the members of this family i.e. AtHK1, (a putative osmosensor, hybrid-type sensory histidine kinase) is known to interact with AtHPt1 (phosphotransfer proteins) in <i>Arabidopsis</i>. Based on predicted rice interactome network (PRIN), the ortholog of AtHK1 in rice, OsHK3b, was found to be interacting with OsHPt2. The analysis of amino acid sequence of AtHK1 showed the presence of transmitter domain (TD) and receiver domain (RD), while OsHK3b showed presence of three conserved domains namely CHASE (signaling domain), TD, and RD. In order to elaborate on structural details of functional domains of hybrid-type HK and phosphotransfer proteins in both these genera, we have modeled them using homology modeling approach. The structural motifs present in various functional domains of the orthologous proteins were found to be highly conserved. Binding analysis of the RD domain of these sensory proteins in <i>Arabidopsis</i> and rice revealed the role of various residues such as histidine in HPt protein which are essential for their interaction.</p></div

Additional file 2: of Mapping the ‘early salinity response’ triggered proteome adaptation in contrasting rice genotypes using iTRAQ approach

Figure S2. DAB staining of the roots of Pokkali and IR64 seedlings in response to 2 h of salinity stress. (TIF 10980 kb

Additional file 3: of Mapping the ‘early salinity response’ triggered proteome adaptation in contrasting rice genotypes using iTRAQ approach

Table S1. Highly differentially expressed proteins (>60 fold) in shoot and root tissues. (XLSX 18 kb

Additional file 1: of Mapping the ‘early salinity response’ triggered proteome adaptation in contrasting rice genotypes using iTRAQ approach

Figure S1. Evan blue staining of the roots of Pokkali and IR64 seedlings in response to 2 h of salinity stress. (TIFF 314 kb

Additional file 6: of A unique bZIP transcription factor imparting multiple stress tolerance in Rice

Table S2. SNPs and InDels analysis of Saltol QTL localized gene OsHBP1b (LOC_Os01g17260) in IR64 and Pokkali genotypes of Oryza sativa L. (DOCX 21 kb

Additional file 2: of A unique bZIP transcription factor imparting multiple stress tolerance in Rice

Figure S2. Assessment of drought stress tolerance of OsHBP1b over-expressing rice seedlings through morphological, physiological and enzymatic analysis. (a) Photograph of WT and OsHBP1b over-expressing plants under drought conditions. Note the prominent difference in root morphology where OsHBP1b over-expressing plants have longer roots with more secondary branches as compared to the WT under drought stress. Bar diagrams showing (b) shoot and root length, (c) fresh weight, (d) Fv/Fm, (e) CAT activity, (f) APX activity and (g) SOD activity measured in the leaves of seedlings after 3 days of drought stress. The data represent means ± SE of three biological replicates. Single asterisk show significant difference at p < 0.005 and double asterisk at p < 0.001. (TIF 8735 kb

Additional file 4: of A unique bZIP transcription factor imparting multiple stress tolerance in Rice

Figure S4. Assessment of ABA responsiveness of OsHBP1b over-expressing rice seedlings through morphological and physiological analysis. (a) Photograph of WT and OsHBP1b over-expressing plants in response to exogenous ABA (5 uM). Note the prominent difference in root morphology where OsHBP1b over-expressing plants have longer roots with more secondary branches as compared to the WT in response to ABA. Bar diagrams showing (b) shoot and root length, (c) fresh weight, (d) Photograph of WT and OsHBP1b over-expressing plants in response to exogenous ABA (10 uM). Bar diagrams showing (e) shoot and root length, (f) fresh weight. The data represent means ± SE of three biological replicates. Single asterisk show significant difference at p < 0.005 and double asterisk at p < 0.001. (TIF 9550 kb