11 research outputs found

    Characterization of infected cells expressing CD11c, CD205 and MOMA-1 in the spleen of IL12p40-deficient BALB/c mice.

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    <p>IL12p40<sup>-/-</sup> STAT6<sup>+/+</sup> BALB/c mice were injected i.n. with 2x10<sup>7</sup> CFU of mCherry-Br. The mice were sacrificed at 28 days post-infection and the spleens were collected and examined by immunohistofluorescence. <b>A</b>, The left panel shows mCherry-Br co-localization with cells expressing CD11c. The middle panel shows mCherry-Br co-localization with cells expressing CD205 and the right panel shows co-localization of CD11c- and CD205-expressing cells. <b>B</b>, The upper panels show distribution of MOMA-1-expressing cells and co-localization with mCherry-Br (left), distribution of DEC205-expressing cells and co-localization with mCherry-Br (middle), and co-localization of MOMA-1- and CD205-expressing cells (right). The panels below are higher magnification views of the same stainings. The panels are color-coded with the text for phalloidin, the antigen examined or mCherry-Br. Scale bar = 50 and 20 μm, as indicated. r.p.: red pulp; w.p.: white pulp. Yellow arrowheads indicate the presence of bacteria. The data are representative of at least two independent experiments.</p

    Characterization of the cell surface phenotype of infected cells in the spleen of IL12p40-deficient BALB/c mice.

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    <p>IL12p40<sup>-/-</sup> STAT6<sup>+/+</sup> BALB/c mice were injected i.n. with 2x10<sup>7</sup> CFU of mCherry-Br. The mice were sacrificed at 28 days post-infection and the spleens were collected and examined by immunohistofluorescence. <b>A</b>. The left panels show the overall distribution of the CD11c-, ERTR9 and MOMA-1-expressing cells in the spleen. The panels to the right of the first ones show mCherry-Br co-localization with negative cells and positive cells for CD11c, ER-TR9 and MOMA-1. The panels are color-coded with the text for phalloidin, the antigen examined or mCherry-Br. Scale bar = 200 and 20 μm, as indicated. r.p.: red pulp; w.p.: white pulp. Yellow arrowheads indicate the presence of bacteria. The data are representative of at least three independent experiments. <b>B</b>. Representative confocal images of mCherry-Br infected CD11c<sup>+</sup> cells. The panels are color-coded with the text for DAPI, mCherry-Br or CD11c. Scale bar = 10 μm, as indicated. w.p.: white pulp.</p

    Analysis of CD205, Arginase1, Fizz1 and CD301 expression on infected spleen cells from IL12p40- and STAT6/IL12p40-deficient BALB/c mice.

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    <p>IL12p40<sup>-/-</sup> STAT-6<sup>+/+</sup> and IL12p40<sup>-/-</sup> STAT-6<sup>-/-</sup> BALB/c mice were injected i.n. with 2x10<sup>7</sup> CFU of mCherry-Br. The mice were sacrificed at 28 days post-infection and the spleens were collected. The figure shown the percentage of mCherry-Br that co-localizes or not with Dec205-, Arg1-, Fizz1- and CD301-expressing cells in the spleen of IL12p40<sup>-/-</sup> STAT-6<sup>+/+</sup> and IL12p40<sup>-/-</sup> STAT-6<sup>-/-</sup> BALB/c mice. The percentage of co-localization between mCherry-Br and positive cells for the antigen in IL12p40<sup>-/-</sup> STAT-6<sup>+/+</sup> mice is indicated. The data are representative of at least two independent experiments.</p

    Course of <i>B</i>. <i>melitensis</i> infection in organs of wild-type (wt), STAT6-, IL12p40- and STAT6/IL12p40-deficient BALB/c mice.

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    <p>The mice were injected intranasally (i.n.) with 2x10<sup>7</sup> CFU of mCherry-Br <i>B</i>. <i>melitensis</i> and sacrificed at the indicated times. The data represent the number of CFU per gram of lung, spleen and liver. Grey bars represent the medians. “n” is the number of mice used. These results are representative of at least two independent experiments. ns, non-significant.</p

    Phenotypical analysis of granuloma in the liver and spleen of wild type and RAG-deficient infected mice.

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    <p>Localization by immunohistofluorescence of (<b>A</b>) F4/80, (<b>B</b>) CD11b, (<b>C</b>) CD11c and iNOS expressing cells in liver and spleen from 120 h mCherry-Br infected wild-type and RAG<sup>−/−</sup> C57BL/6. Mice were injected i.p. with 10<sup>8</sup> CFU of mCherry-Br. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.</p

    Phenotypical analysis of granuloma in the liver of wild type and MyD88-deficient infected mice.

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    <p>Colocalization by immunohistofluorescence of CD11c (<b>A</b>), F4/80 (<b>B</b>), Ly-6G (<b>C</b>) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88<sup>−/−</sup> C57BL/6. Mice were injected i.p. with 10<sup>8</sup> CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.</p

    Phenotypical characterization of <i>B. melitensis</i> infected cells in the spleen.

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    <p>Wild-type C57BL/6 and BALB/c mice were injected i.p. with PBS or 10<sup>8</sup> CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. Data indicate the percentage of mCherry-Br that colocalizes with (<b>A</b>) CD90.2-, B220-, Ly6G-, MOMA-1-, F4/80-, CD11c-, (<b>B</b>) MHCII-, iNOS-, CD11b-expressing cells at the selected time in C57BL/7 mice. (<b>C</b>) Comparison at 120 h p.i. between C57BL/6 and BALB/c mice. The bars are the mean±SD from at least 3 spleen sections per spleen from 5 mice.</p

    Red pulp localization of <i>B. melitensis</i> at the onset of infection.

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    <p>Wild-type C57BL/6 mice were injected i.p. with PBS or 10<sup>8</sup> CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. <b>A</b>, Positioning of mCherry-Br in the spleen of mice at 3 and 24 h p.i. The inset areas in the middle panels are shown in the bottom panels. <b>B</b>, Immunoflurescence analysis of F4/80, MOMA-1, Ly-6G, and CD11c expressing cells and mCherry-Br in the spleen of mice at 3 h p.i. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp; m.z.: marginal zone. Data are representative of at least 3 independent experiments.</p

    CD11b<sup>+</sup> granulomas in the spleen and liver of wild type and MyD88-deficient infected mice.

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    <p>Mice were injected i.p. with PBS (control) or 10<sup>8</sup> CFU of mCherry-Br. The figure presents the localization by immunohistofluorescence of CD11b<sup>hi</sup> and iNOS expressing cells in spleen (<b>A</b>) and liver (<b>B</b>) from control and 120 h mCherry-Br infected wild-type and MyD88<sup>−/−</sup> C57BL/6. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp. Data are representative of at least 3 independent experiments.</p

    <i>B. melitenis</i> infects CD11c<sup>+</sup> CD205<sup>+</sup> or MOMA-1<sup>+</sup> cells in spleens of chronically infected Il-12-deficient BALB/c mice.

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    <p>Wild-type and Il-12p40<sup>−/−</sup> BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. <b>A</b> & <b>B</b>, Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12<sup>−/−</sup> BALB/c mice infected with 10<sup>6</sup> CFU 12 days p.i.. <b>C</b>, Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10<sup>8</sup> CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.</p
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