8 research outputs found

    Raw gene copy numbers detected in a WWTP sample (copy number/ml).

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    <p>A - 16S rRNA gene in inflow (IF) and effluent (EF). Assay1 was used only for samples from large WWTP (Helsinki) from Winter 2010 to Autumn 2011; B–Antibiotic resistance genes (ARGs). Statistical significance between inflow wastewater and effluent samples: *** - p<0.01; *0.03>p>0.01. For the pairs not marked the statistical difference between inflow and outflow was statistically insignificant. The line in each box marks the median and boxes: 25th and 75th percentiles; whiskers: 5th and 95th percentiles and outliers ±1.5 * IQR. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103705#pone.0103705.s001" target="_blank">Figure S2 in File S1</a> for abundances of same genes presented by each sampling event.</p

    Primers used for detecting the target genes and the melting temperatures (Tm) used for primers.

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    <p>qPCR amplification efficiency is given for 16S RNA gene and for ARGs, R<sup>2</sup> of the linear range of standards was always >0.99.</p

    Sampling sites and their descriptions.

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    a<p>Mean values of depth, temperature (T) and pH were measured from bottom seawater at sampling sites located in the archipelago area in the northern Baltic Sea.</p

    Correlation analysis.

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    <p>Linear regression model with log-transformed variables between the <i>intI1</i> and <i>sul1</i> gene copy numbers in the sediments below the northern Baltic Sea farms (<i>F<sub>1,22</sub></i> = 19.39; <i>P</i> = 0.000225; <i>R<sup>2</sup></i> = 0.47). Each point represents the average ratio of a gene copy number normalized to the 16S rRNA gene copy number in every sediment sample. The blue line indicates the regression model and the grey area the 95% confidence intervals.</p