6 research outputs found

    Growth rate changes in sea ice brine algae based on chl-<i>a</i> and biovolume.

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    <p>a: Growth rate of the brine algal incubations with varying pH, based on changes in chl-<i>a</i> and biovolume, in November 2012, experiment 1. b: Growth rate of the brine algal incubations (chlorophyll a and biovolume) with constant pH and varying CO<sub>2</sub> in November 2012, experiment 2. Vertical grey line signifies initial pH and pCO<sub>2</sub>.</p

    Maximum quantum yield (F<sub>v</sub>/F<sub>m</sub>) of brine algae with varying pH and pCO<sub>2</sub>.

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    <p>a: Maximum quantum yield (F<sub>v</sub>/F<sub>m</sub>) of the brine algal incubations with varying pH in November 2012, experiment 1. b: Maximum quantum yield (F<sub>v</sub>/F<sub>m</sub>) of the brine algal incubations with constant pH and varying CO<sub>2</sub> in November 2012, experiment 2. Vertical grey line signifies initial pH and pCO<sub>2</sub>.</p

    Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers

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    The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX
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