Iyer Laboratory Solid Tissue Chromatin Immunoprecipitation Protocol

A detailed protocol on how to perform chromatin immunoprecipitation in solid tissue samples. This protocol is used routinely in the Iyer lab.Molecular Bioscience

How do Companies Communicate with Nonexempt/Offline/Non-Desk Employees and Keep Them Confident and Engaged During Times of Organizational Change?

[Excerpt] Companies have had to evolve continuously to remain competitive in today’s marketplace. Especially in industries with blue-collar workers, automation and other forms of innovation have become looming stress points to many employees. Maintaining employee engagement is a key element of any successful change initiative. Strong leaders are needed to be able to inform, engage, enable, and build trust within the organization, to promote confidence and keep workers satisfied. While much research has been conducted on the engagement of white-collar workers, not much information is available for offline blue-collar workers, who are a part of a growing workforce. If not careful, these crucial employee groups, who are constantly on the move and do not use traditional communication technologies in their daily jobs, can feel overlooked and become disengaged

Recurrence plots of sunspots, solar flux and irradiance

The paper shows the recurrence and cross recurrence plots of three time series, concerning data of the solar activity. The data are the sunspot number and the values of solar radio flux at 10.7 cm and of solar total irradiance, which are known as highly correlated. To compare the series, the radio flux and irradiance values are monthly averaged. Recurrence plots display the oscillating behaviour with remarkable features. Moreover, cross recurrence plots help in identifying time lags between the sunspot number maximum and the maximum of radio or irradiance signals, in circumstances where the data values are highly dispersed. Image processing is useful too, in enhancing the monitoring. An interesting behaviour is displayed by cross recurrence plots of irradiance, which are not symmetric with respect to the line of identity

Digital Restoration of Ancient Papyri

Image processing can be used for digital restoration of ancient papyri, that is, for a restoration performed on their digital images. The digital manipulation allows reducing the background signals and enhancing the readability of texts. In the case of very old and damaged documents, this is fundamental for identification of the patterns of letters. Some examples of restoration, obtained with an image processing which uses edges detection and Fourier filtering, are shown. One of them concerns 7Q5 fragment of the Dead Sea Scrolls

Methods for Computing Normalisations of Affine Rings

Our main purpose is to give multiple examples for using the available implementations for computing the normalization of an affine ring, computing the minimial generators of the normalization as an algebra over the original ring and integral closures of ideals. Some such examples have been published for Singular, but not for Macaulay 2 and we present both in this paper. We also briefly describe the implementations.Comment: To Appear in "Advances in Algebra and Geometry (University of Hyderabad Conference 2001)" . Includes extensive examples. 17 page

Analysis of pilot data assessing vaccine hesitancy in an urban clinic setting

Widespread use of childhood vaccination has significantly reduced the burden of childhood disease, however a subset of parents are choosing to delay or refuse available vaccines. This study analyzed data collected from a large surveillance study to examine the parents’ attitudes about vaccines and the prevalence of vaccine hesitancy (vaccine delay and refusal) in an urban clinic population. The parents of 961 children attending the Boston Medical Center Pediatrics department participated in the study. Parental responses to four vaccine questions were used to assess vaccine attitudes. Log-Binomial Regression models were used to evaluate the relationship between comorbidity status and birth order with vaccine delay or refusal. In this study population, parents reported generally positive attitudes toward vaccination. The majority (87%) believed that vaccines were necessary to protect their child. Approximately 16% of parents reported that had delayed or refused a vaccine and 24% of parents indicated that they did not believe or were unsure if other parents vaccinating their children. When asked the reason for their choice, parents who only delayed frequently cited logistic concerns like a missed appointment, whereas parents who refused more often cited personal beliefs, such as concerns that their child could become ill from vaccination. Finally, parents of children with a comorbidity were more likely to refuse a vaccine than parents of children without comorbidity (Adjusted RR=1.8, 95% CI: 1.1, 2.9). While parents were generally positive toward vaccines, for the small portion of parents refusing vaccines, further work could help to better explain their motivations

Moral Hedging and Responding to Reasons

In this paper, I argue that the fetishism objection to moral hedging fails. The objection rests on a reasons-responsiveness account of moral worth, according to which an action has moral worth only if the agent is responsive to moral reasons. However, by adopting a plausible theory of non-ideal moral reasons, one can endorse a reasons-responsiveness account of moral worth while maintaining that moral hedging is sometimes an appropriate response to moral uncertainty. Thus, the theory of moral worth upon which the fetishism objection relies does not, in fact, support that objection

What do Newer Entrants into the Workforce Really Want?

Companies have had to evolve continuously to remain competitive in today’s marketplace. Technological advancements, shifting in social dynamics, and the changing demographics all impact how companies attract and retain talent. As more information becomes available on the internet, and as more generations are in the workforce at the same time, it becomes increasingly challenging for companies to develop cost effective and attractive benefits schemes