Use of Phenols, Peroxidase and Polyphenoloxidase of Seed to Quantify Resistance of Cotton Genotypes to Damping-off Incited by Fusarium oxysporum

A greenhouse test was conducted in 2011 and 2012 growing seasons at Giza Agricultural Research Station to evaluate the reaction of six cotton genotypes to damping-off incited by Fusarium oxysporum. Damping-off incidence on the genotypes ranged from 70-88%. In general, the genotypes could be divided into highly susceptible, susceptible, and moderately susceptible. Data for damping-off incidence and level or activity of some biochemical components (phenols, peroxidase, and polyphenoloxidase) were entered into a computerized linear regression analysis. The analysis contrasted seven predictive models by using the biochemical components, singly or in combination, as biochemical predictors. It was evident that models nos. 2 and 6 were the best models for predicting incidence of damping-off. The superiority of these models was attributed to their high RІ values (0.748 and 0.902, respectively) and the significance of their F. values (P = 0.026 and P = 0.031, respectively). The results of the present study suggest that peroxidase alone or both peroxidase and polyphenoloxidase, which may or may not parts of damping-off resistance mechanisms, can be used as biochemical markers to predict resistance to damping-off incited by F. oxysporum

Effectiveness of zinc supplementation on diarrhea and average daily gain in pre-weaned dairy calves: A double-blind, block-randomized, placebo-controlled clinical trial.

The objective of this clinical trial was to evaluate the effectiveness of zinc supplementation on diarrhea and average daily weight gain (ADG) in pre-weaned dairy calves. A total of 1,482 healthy Holstein heifer and bull calves from a large California dairy were enrolled at 24 to 48 hours of age until hutch exit at approximately 90 days of age. Calves were block-randomized by time to one of three treatments: 1) placebo, 2) zinc methionine (ZM), or 3) zinc sulfate (ZS) administered in milk once daily for 14 days. Serum total protein at enrollment and body weight at birth, treatment end, and hutch exit were measured. Fecal consistency was assessed daily for 28 days post-enrollment. For a random sample of 127 calves, serum zinc concentrations before and after treatment and a fecal antigen ELISA at diarrhea start and resolution for Escherichia coli K99, rotavirus, coronavirus, and Cryptosporidium parvum were performed. Linear regression showed that ZM-treated bull calves had 22 g increased ADG compared to placebo-treated bulls (P = 0.042). ZM-treated heifers had 9 g decreased ADG compared to placebo-treated heifers (P = 0.037), after adjusting for average birth weight. Sex-stratified models showed that high birth weight heifers treated with ZM gained more than placebo-treated heifers of the same birth weight, which suggests a dose-response effect rather than a true sex-specific effect of ZM on ADG. Cox regression showed that ZM and ZS-treated calves had a 14.7% (P = 0.015) and 13.9% (P = 0.022) reduced hazard of diarrhea, respectively, compared to placebo-treated calves. Calves supplemented for at least the first five days of diarrhea with ZM and ZS had a 21.4% (P = 0.027) and 13.0% (P = 0.040) increased hazard of cure from diarrhea, respectively, compared to placebo-treated calves. Logistic regression showed that the odds of microbiological cure at diarrhea resolution for rotavirus, C. parvum, or any single fecal pathogen was not different between treatment groups. Zinc supplementation delayed diarrhea and expedited diarrhea recovery in pre-weaned calves. Additionally, zinc improved weight gain differentially in bulls compared to heifers, indicating a research need for sex-specific dosing

Water use efficiency and maximizing profitability of grain sorghum production in the Texas Panhandle

The reduction in the availability of irrigation water and the increase in pumping costs resulting from the decline in the Ogallala Aquifer make good management decisions more critical for the survival of the farm firm and the success of the agricultural sector in the Texas Panhandle. Response functions for irrigation and percentage potential evapotranspiration (PET) in the production of grain sorghum are estimated. The response functions are transferred into value product functions and combined with an irrigation energy cost function to determine the profit maximizing irrigation strategy. Three management decision variables; total water available, the level of irrigation and the water to meet crop ET requirements are evaluated. Grain sorghum yield, natural precipitation, irrigation, soil moisture content, potential evapotranspiration, and percent potential evapotranspiration (PET) data, collected over the period from 1998 through 2007 by commercial producers participating in the AgriPartners program are used to estimate the response functions. Results indicate that the optimum level of irrigation increases as the price of sorghum increases and decreases as the price of natural gas increases.Grain sorghum, ET, maximizing profit, irrigation efficiency, input use optimization, water conservation, Ogallala Aquifer, Texas Panhandle., Farm Management, Production Economics, Productivity Analysis, Resource /Energy Economics and Policy, Q12, Q15, Q25, Q32, and Q34,

The uncertain representation ranking framework for concept-based video retrieval

Concept based video retrieval often relies on imperfect and uncertain concept detectors. We propose a general ranking framework to define effective and robust ranking functions, through explicitly addressing detector uncertainty. It can cope with multiple concept-based representations per video segment and it allows the re-use of effective text retrieval functions which are defined on similar representations. The final ranking status value is a weighted combination of two components: the expected score of the possible scores, which represents the risk-neutral choice, and the scores’ standard deviation, which represents the risk or opportunity that the score for the actual representation is higher. The framework consistently improves the search performance in the shot retrieval task and the segment retrieval task over several baselines in five TRECVid collections and two collections which use simulated detectors of varying performance

Investigation of Anaplasma marginale Seroprevalence in a Traditionally Managed Large California Beef Herd.

Recent observations by stakeholders suggested that ecosystem changes may be driving an increased incidence of bovine erythrocytic anaplasmosis, resulting in a reemerging cattle disease in California. The objective of this prospective cohort study was to estimate the incidence of Anaplasma marginale infection using seroconversion in a northern California beef cattle herd. A total of 143 Black Angus cattle (106 prebreeding heifers and 37 cows) were enrolled in the study. Serum samples were collected to determine Anaplasma marginale seroprevalence using a commercially available competitive enzyme-linked immunosorbent assay test kit. Repeat sampling was performed in seronegative animals to determine the incidence density rate from March through September (2013). Seroprevalence of heifers was significantly lower than that of cows at the beginning of the study (P < 0.001) but not at study completion (P = 0.075). Incidence density rate of Anaplasma marginale infection was 8.17 (95% confidence interval: 6.04, 10.81) cases per 1000 cow-days during the study period. Study cattle became Anaplasma marginale seropositive and likely carriers protected from severe clinical disease that might have occurred had they been first infected as mature adults. No evidence was found within this herd to suggest increased risk for clinical bovine erythrocytic anaplasmosis

Environmental sampling to assess the bioburden of Mycobacterium avium subspecies paratuberculosis in drylot pens on California dairies.

Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental sampling protocol to quantify MAP in fecal slurry was developed based on an existing protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental sampling protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental sampling simultaneously by different collectors. In contrast, the reliability of environmental sampling at different days was 65%, which was similar to the reliability for sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental sampling protocol along with qPCR testing of environmental samples from drylot pens

MHD simulations of disk-star interaction

We discuss a number of topics relevant to disk-magnetosphere interaction and how numerical simulations illuminate them. The topics include: (1) disk-magnetosphere interaction and the problem of disk-locking; (2) the wind problem; (3) structure of the magnetospheric flow, hot spots at the star's surface, and the inner disk region; (4) modeling of spectra from 3D funnel streams; (5) accretion to a star with a complex magnetic field; (6) accretion through 3D instabilities; (7) magnetospheric gap and survival of protoplanets. Results of both 2D and 3D simulations are discussed.Comment: 12 pages, 10 figures, Star-Disk Interaction in Young Stars, Proceedings of the International Astronomical Union, IAU Symposium, Volume 243. See animations at http://astro.cornell.edu/~romanova/projects.htm and at http://astro.cornell.edu/us-rus

"Propeller" Regime of Disk Accretion to Rapidly Rotating Stars

We present results of axisymmetic magnetohydrodynamic simulations of the interaction of a rapidly-rotating, magnetized star with an accretion disk. The disk is considered to have a finite viscosity and magnetic diffusivity. The main parameters of the system are the star's angular velocity and magnetic moment, and the disk's viscosity, diffusivity. We focus on the "propeller" regime where the inner radius of the disk is larger than the corotation radius. Two types of magnetohydrodynamic flows have been found as a result of simulations: "weak" and "strong" propellers. The strong propeller is characterized by a powerful disk wind and a collimated magnetically dominated outflow or jet from the star. The weak propeller have only weak outflows. We investigated the time-averaged characteristics of the interaction between the main elements of the system, the star, the disk, the wind from the disk, and the jet. Rates of exchange of mass and angular momentum between the elements of the system are derived as a function of the main parameters. The propeller mechanism may be responsible for the fast spinning-down of the classical T Tauri stars in the initial stages of their evolution, and for the spinning-down of accreting millisecond pulsars.Comment: 18 pages, 16 figures, ApJ (accepted), added references, corrected typos; see animation at http://astrosun2.astro.cornell.edu/us-rus/disk_prop.ht